جستجوی مقالات مرتبط با کلیدواژه « Antimicrobial resistance gene » در نشریات گروه « کشاورزی »

Enterococci cause meat and environmental contamination during slaughter time. In this study, virulence and antimicrobial resistance (AMR) characteristics of enterococci isolated from chickens were determined. A total of 107 cloacal swabs of chickens were inoculated onto Slanetz and Bartley agar and incubated at 37ºC for 24-48 h. Gram staining, catalase, and hemolytic tests were done. AMR was determined using the disc diffusion technique against twelve antimicrobials. Molecular detection of AMR genes: blaZ, aphA, aacA-aphD, ermB, tetL, tetM, and vanC, and virulence factors: agrBEfs, efaAEfs, esp, gelE, and hyl were done on selected isolates using PCR. Ninety-five isolates were Enterococcus species. The isolates showed resistance to tetracycline, cefoxitin, amoxicillin, and imipenem and possessed tetL, tetM, ermB, aphA, vanC, aaca-aphD resistance and gelE, agrBef, efaAfs, espfs and hyl virulence genes. This is the first detection of AMR and virulence genes in multi-drug resistant enterococci among chickens in the locality. These enterococci could constitute a reservoir of virulence and resistance properties which are of animal and public health concern.