جستجوی مقالات مرتبط با کلیدواژه « ضدویروسی » در نشریات گروه « پزشکی »

Viruses are sub-microscopic infectious agents that cause many diseases in humans, including several fatal infections that have emerged in the recent past. Rapid and accurate diagnosis of viral infections in the early stages is crucial in preventing the emergence of the pathogen. However, most of the currently available diagnostic methods are laborious, time-consuming, and less sensitive. On the other hand, the limited availability of antiviral treatments and the adverse effects associated with traditional antiviral drugs suggest the necessity of developing new therapeutic strategies to combat viral infections. In recent years, various types of nanomaterials have been investigated for the diagnosis and treatment of viral infections. In this review article, some of the nanotechnology-based approaches that have been developed for rapid and high-quality detection of viral infections, as well as antiviral therapeutic strategies related to nanomaterials, are also discussed with examples. Viral infections have emerged as a major threat to global health, especially in recent years. However, only a limited number of diagnostic tools and antiviral treatments are available to combat these viral infections. New approaches based on nanotechnology can provide a better platform for detecting viral infections in a short time with high sensitivity compared to existing viral detection methods. Also, nanomaterials may help overcome issues such as low solubility and bioavailability, rapid release, development of resistance, and adverse effects often associated with conventional antiviral therapies. Therefore, nanotechnology appears as a new and promising tool in the effective management of viral infections in future medicine.

Nowadays, the treatment of viral diseases such as Herpes simplex virus type 1 with chemical drugs is one challenge of medical knowledge due to the appearance of drug resistance in the virus. So, the need is for new antiviral drugs. In this regard, we can use the medicinal application of plants which has been found in human life lots. In this study, antiviral effect of Thymus kotschyanus plant was investigated on HSV-1 multiplication.
Thymus kotschyanus plant was extracted with decoction method to obtain aqueous extract. This extract was screened for its cytotoxicity on Hep-2 cell line by CPE assay at concentrations 50-1000 μg/ml. Antiviral properties of the plant extract was determined by cytopathic effect inhibition assay at one, two and three hours after inoculation of HSV-1.
Thymus kotschyanus plant had not toxic effect at nontoxic concentrations to use. Findings indicated most antiviral effect of plant extract after an hour of virus inoculation that decreased at two and three hours.
Thymus kotschyanus extract exhibited very well antiviral effect on HSV-1 at nontoxic concentrations to use. Further research is needed to find effect mechanism of this plant which be used in conformation of antiviral drugs.

The antimicrobial properties of cyanobacteria was recently highlighted. The aim of this study was to evaluate the activity and antiviral properties of cyanobacteria collected from oil-polluted areas of southern Iran.

In the present study، aqueous and methanolic extracts of five species ofcyanobacteria were prepared. Cytotoxic effects ofcyanobacteria on (lymphoblast cell line) LCL and Hela cell lineswere evaluated. Using MTT assay and medium only served as a negative control group. Hela and LCL cells also infected by human papilloma virus (HPV) and Epstein-Barr virus (EBV) respectively and were exposed to the cyanobacteria extracts to examine the anti-viral effects of extracts. DNA was extracted and the viral gene encoding envelope was also carried out by PCR. The samples were assessed in order to examinethat viruses are capable of successfully infecting and lysing embedded colonies. The data were analyzed using Two-way ANOVA tests.

The results indicated that cyanobacteria extract had cytotoxic effects on LCL and HeLa cell lines. In addition viral load gene was significantly decreased in treated cells.

According to the significant antiviral effects of alcoholic and aqueous extracts of cyanobacteria. These extracts may used for a variety of viral therapies.

Several species of the genus Euphorbia (Euphorbiaceae) have been tested for their efficiency as anti-viral and anti-tumor agents. Herpes Simplex Viruses (HSV) are pathogenic to human. Antiviral drugs are mostly weak or are associated with side effects. Medicinal plant products have been used as folk remedies for different kinds of ailments including viral diseases. The objective of this study was to compare the phytochemical study and anti-viral effect evaluation of methanolic extract with fractions of aerial parts of euphorbia spinidens. In this experimental and laboratory research the methanolic extract of aerial parts of E. spinidens with maceration method was subjected to solvent partitioning and afforded chloroform, butanol and hexane fractions.? -carotene-linoleate model system, folin-Ciocalteu method and aluminum chloride colorimetric method, respectively, were employed for evaluation of antioxidant activity, total phenolic, flavonoid and flavonol contents of the extract and fractions of this plant. The cytotoxic and anti-viral effects of methanolic extract and fractions were determined using MTS assay and the CC50) 50% cytotoxic concentration(and EC50 (50% effectiveness concentration) were evaluated on Herpes Simplex Virus Type-1. The highest antioxidant activity and phenolic and flavonoid and contents were in buthanol fraction (59.33±0.576, 99±1, 70±1), methanolic extract (44±1, 70±1, 49.96±0.996), chloroform fraction (33.33±0.996, 35.33±0.577, 25.33±0.996) and hexane fraction (19.66±1.146, 27±1, 9.33±1.628) respectively. The highest CC50 was in hexan fraction with 9.92± 0.072 mg/ml and the lowest CC50 was in methanolic extract with 5.072±0.063 mg/ml. Also, the highest anti viral activity revealed in the butanol fraction (SI: 28.125), methanolic extract (SI: 15.85), chloroform fraction (SI: 8.44) and hexane fraction (SI: 2.07), respectively. Based on the results, methanolic extract and buthanol, chloroform and hexane fractions of E. spinidens show anti HSV-1 activity. Antiviral effect observed can be attributed the presence of various secondary metabolites, particularly polyphenols, flavonoids, terpenes and saponins.