جستجوی مقالات مرتبط با کلیدواژه « محتوای فلاونوئیدی » در نشریات گروه « پزشکی »

Nepeta crispa Willd. (Lamiaceae) is a medicinal plant endemic to Iran with numerous ethnobotanical uses.

The current study focuses on the quality control of N. crispa growing in Hamadan province.

Hydro-distilled essential oil from aerial parts of N. crispa was obtained using the Clevenger apparatus. Four samples of N. crispa distillate were purchased from various locations. Methanol extracts from aerial parts of this plant were obtained by the maceration method. Micromorphological characteristics, antioxidant properties, and chemical constituents of N. crispa essential oil and distillates were evaluated. Determination of the total phenols and flavonoid contents of the extract was performed.

GC/MS analysis revealed that the major components of N.crispa essential oil were 1,8-cineole (70.93 %), nepetalactones (5.29 %), and β-pinene (5.5 %). The volatile constituents of one of the distillates resemble the essential oil of N. crispa. The extract from aerial parts of N. crispa consistently exhibited more anti-oxidative power than its essential oil with an IC50 of 2.792 ± 0.385 mg/ml. The results from assays for total phenols and flavonoids revealed that this plant possesses phenolic and flavonoid content of 69.24 ± 3.34 µg GAEs/mg and 12.01 ± 0.26 µg QEs/mg of extract, respectively. Covering, glandular trichomes, diacytic stomata, and spiral vessels were seen in microscopic studies.

Our results suggest that N. crispa possess considerable anti-oxidative potential with relatively high phenolic and flavonoid content in which 1,8-cineole is the major component. Due to the rarity of this plant, there are fraudulent specimens among its commercial distillates.

The use of medicinal plants has been one of the most common treatments since ancient times. Various plants are used in traditional and modern medicine, due to their antioxidant, antimicrobial and anticancer properties, and other biological potentials.

In this study, the aerial parts of four species of Salvia including two populations of S. reuteriana Boiss., two populations of S. limbata C.A.Mey. and one population of each S. syriaca L. and S. ceratophylla L. species from Kashan region have been investigated.

The antioxidative activity of the methanol extracts of plant samples were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The total phenol and flavonoid contents were determined via the Folin-Ciocalteu and aluminum chloride methods, respectively. The cytotoxic effects of samples on HeLa cells were determined through MTT assay.

Based on the results of DPPH assay, IC50 levels of methanolic extracts were in the range of 39.08 ± 0.15 to 163.77 ± 0.63 μg/ml, and total phenol contents were in the range of
42.7 ± 3.09 to 105.8 ± 1.15 μg/mg. In addition, the flavonoid contents ranged from 30.07 ± 2.52 to 82.46 ± 2.2 μg/mg. There was a direct relationship between antioxidant activity and phenol compound contents. According to our study, the methanolic extracts of Salvia species showed toxicity effects on HeLa cells.

The extracts of two populations of S. reuteriana were associated with the highest cytotoxicity, compared to other species of Salvia. Furthermore, all examined extracts exhibited weak to moderate antioxidant activities. The S. ceratophylla extract was associated with the highest antioxidant activity.