جستجوی مقالات مرتبط با کلیدواژه « آنقوزه » در نشریات گروه « پزشکی »

Ferula assa-foetida is an endemic medicinal plant of Iran. Its oleo-gum resin or assafoetida gum (AG) is an important herbal product whose standardization is important to ensure effectiveness and safety.

Due to the lack of accurate and complete data about Iranian AG, the objective of this study is to extend the available knowledge by reporting some physicochemical and phytochemical properties of an Iranian AG sample.

Organoleptic properties were determined by sensory evaluation. Total ash, acid-insoluble ash, dry residue as well as total phenolic and flavonoid content were measured by accepted methods to determine some physicochemical and phytochemical properties of AG. Analysis of its essential oil by GC/MS as well as microbial load assessment were performed.

The AG was a yellowish brown mass with a sulfur-like pungent odor similar to garlic, with total ash and acid-insoluble ash of 2.83 % and 2.06 %, respectively, and a dry residue of 3.43 %. Total phenol content was 4.15 ± 0.26 mg gallic acid equivalent/g in dry AG. (E)-1-Propenyl sec-butyl disulphide, (Z)-1-propenyl sec-butyl disulphide, and 2,4-thiazelidinedione were the major essential oil component of this oleo-gum resin. Microbial load was in acceptable range according to Pharmacopeia.

The current findings provide data on some of the physicochemical and phytochemical properties of Iranian AG which is applicable for completing current knowledge. Although more information is needed for establishing Iranian AG standardization criteria, which will be obtained from more extensive researches.

Leishmaniasis is a complex disease with a broad spectrum of clinical features, usually divided into cutaneous leishmaniasis (CL), muco-cutaneous leishmaniasis (MCL), and visceral leishmaniasis (VL). Plant extracts or plant-derived compounds are likely to provide a valuable source of new medicinal agents. In endemic countries, a number of traditional plants are commonly used to treat infectious conditions. Advances in the research of natural products for the treatment of leishmaniasis have been recently reviewed. To evaluate, anti-Leishmanial activity of three plant extracts on Leishmania major promastigotes as compared to a trivalent antimonial compound (tartar emetic), in vitro. promastigote stages of L.major (MRHO/IR/75/ER) were transferred to RPMI-1640 medium, supplemented with 10% fetal calf serum (FCS) and antibiotics then grown at 25±2°C. The biological activity of plant extracts in comparison to potassium antimonyl tartrate [Sb(III)] on L.major promastigotes were assessed by using a MTT assay. The optical density (OD) due to cleavage of the tetrazolium salt MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] into a colored product formazan by the parasite was measured by ELISA reader. The IC50 values (50% inhibitory concentrations) were determined, accordingly. All experiments were repeated in duplicate. plant extracts and tartar emetic inhibited the growth of promastigote forms of L.major in vitro after 72 hour of incubation and drug control had a 50% inhibitory concentration (IC50) of 4.7 µg/ml, and IC50 values of plant extracts Artemisia aucheri, Ferula asa-foetida and Gossypium hirsutum were 7.5, 5.9 and 3.6 µg/ml, respectively. Although Gossypium hirsutum was more effective than others, but all extracts had profound effect on promastigotes of L.major. Plant extracts including Artemisia aucheri, Ferula asa-foetida and Gossypium hirsutum have anti-leishmanial effects in vitro. Further works are required to evaluate the exact effect of these extracts on Leishmania species in animal models.