جستجوی مقالات مرتبط با کلیدواژه "استئوبلاست ها" در نشریات گروه "پزشکی"

The fetus is the source of many stem cells that have great potential in repairing and regenerating different tissues and can differentiate into various types of cells including osteoblast and adipocyte. However, less attention has been paid to the isolation and differentiation ability of these cells in the culture medium. Therefore, the present study aimed to determine the isolation method, proliferation, and differentiation of fetal mesenchymal stem cells (fMSCs) derived from rat fetuses.

In this experimental study, fMSCs were isolated from 15-day-old rat fetuses using trypsin enzymatic digestion and they were cultured up to 3th passage. Then, the fMSCs were characterized by flow cytometry and differentiated into osteoblast and adipocyte cells. Cell proliferation and expression of osteogenesis- and adipogenesis-related genes were investigated in differentiated cells.

Cell surface markers of fMSCs at passage 3 were positive for CD105 and CD90 and negative for CD45 and CD34. These cells exhibited a fibroblast-like morphology. These cells had calcium deposition in osteogenic differentiation and accumulation of fat droplets in adipogenic differentiation. The osteogenesis-related genes including osteonectin, ALP, BMP-6, and Runx-2, and adipogenesis-related genes such as PPARγ and CREBBP were expressed in osteogenic and adipogenic differentiation cultures, respectively.

According to the findings of the present study, it seems that mesenchymal stem cells isolated from rat fetuses have the potential to proliferate and differentiate into osteoblast or adipogenic lineages.

The correlation between hematopoietic stem cells (HSCs) and the cells comprising the niche especially osteoblast cells is critical for maintaining stem cell activities. Yet little evidence supports the concept that HSCs regulate development of the niche. If true، it may explain why many hematopoietic defects are accompanied by changes in the osseous architecture and provide new therapeutic targets for regulating bone formation. In this exprimental study، we cocultured bone marrow mesenchymal stem cells (MSCs) with umbilical cord blood HSCs in stem span media for 3 days. Then MSCs were differentiated to osteoblast cells by using osteogenesis kit. Evaluation of osteogenic differentiation of MSCs in different days came out to be: expression of osteopontin and osteocalsin (RT-PCR on days 4 and 6)، expression of CD90 (flow cytometry on day 6)، expression of alkaline phosphatase enzyme (alkaline phosphatase staining) and mineralization of MSCs (alizarin red staining on day 10). Our results showed early expression of osteopontin and osteocalsin in HSCs coculture with MSCs. These findings support the role of HSCs in induction of osteoblastic differentiation of MSCs. Decrease in the expression of CD90، higher activation in cell alkaline phosphatase enzyme، and mineralization confirmed the results. In the field of human stem cells، our ex vivo findings demonstrated that HSCs can enhance differentiation of MSCs toward osteoblast cells thereby participating in formation of their niche.