جستجوی مقالات مرتبط با کلیدواژه « انتروکوکوس فاسیوم » در نشریات گروه « پزشکی »

Increasing food consumption outdoors in different societies has raised the risk of transmission of foodborne pathogens as a global health problem. Molecular typing methods such as REP-PCR produced DNA profiles for differentiation and characterization of pathogenic strains. The aim of this study was to evaluate the accuracy of molecular fingerprinting method based on repeated sequences (rep-PCR) to determine the affinities between different of Enterococcus fascia isolated from beef meat sold in Shahrekord retail markets.

In this descriptive cross-sectional study, 80 meat samples were examined by biochemical and molecular methods for the presence of Enterococcus fascia and the molecular patterns were determined on the basis of the existence or absence of DNA fragments were separated according to their size by agarose gel electrophoresis. By using a computer program NTSYS version2.02e, dendrogram were drawn.

Of the 80 samples, 66 (82.5%) were identified as Enterococcus, while 30 (45.45%) were Enterococcus fascia. Based on Genetic Classification by Rep-PCR, 30 isolates of Enterococcus fascia were included in 18 profiles. Placement of the studied isolates in several subgroups showed the acceptable discrimination power of Rep-PCR technique in genotyping of Enterococcus fascia.

 The results of this study showed that Rep-PCR is a simple, fast, and method with high dispersal ability to characterize the genetic diversity of Enterococcus fascia strains.

Glycopeptide resistance genes can be transmitted to clinical strains from gram-positive environmental bacteria and the normal flora. The aim of this study was to evaluate the prevalence of vancomycin and teicoplanin-resistant Enterococcus faecalis and Enterococcus faecium isolates in hospital environment and fecal samples of healthy individuals.

Human stool and hospital environment samples were collected and inoculated on selective M-enterococcus agar medium. Then, E. faecalis and E. faecium were identified using phenotypic and molecular methods. Antibiotic susceptibility pattern of the isolates against teicoplanin and vancomycin was determined by micro-broth dilution method. Data were analyzed in SPSS applying Chi-square test.

From 145 isolates, E. faecalis and E. faecium were detected in 84 (54.93%) and 61 (42.07%) isolates, respectively. One (1.19%) E. faecalis isolate and 4 (6.56%) E. faecium isolates were resistant to vancomycin, while 4 (6.56%) E. faecium isolates were found to be resistant to teicoplanin. The minimum inhibitory concentrations of 50 (MIC50) and 90 (MIC90) for vancomycin and teicoplanin in E. faecalis and E. faecium were 4 and 16 μg/ml, respectively.

The MIC results of vancomycin and teicoplanin showed that most of enterococci isolates studied were sensitive to these two antibiotics. Therefore, their use should be closely monitored to prevent resistance.

Lactic acid bacteria )LAB) are a group of gram-positive, non-spore forming, cocci or rod shaped, catalase-negative organisms, which are generally recognized as safe (GRAS). The LAB can cause changes in the food flavor and texture. Enterococci are a group of LAB capable of producing antimicrobial peptides.

This experimental study was conducted to identify and isolate genes encoding antimicrobial peptides from an E. faecium LUB950217 strains isolated from oak tree. In doing so, genomic DNA was extracted from E. faecium strains and polymerase chain reaction (PCR) analysis was performed using specific primers. Amplified PCR products were sequenced by Sanger sequencing. Bioinformatic analysis was carried out and the peptide was modeled. Both DNA and protein Blast search confirmed that the PCR product encoded an antimicrobial peptide, known as LUB950217 enterocin with 36 amino acids of 4658.28 Da molecular mass. The isolated enterocin peptide had 86-100% similarity to other known enterocins in the peptide data bases. The LUB950217 enterocin was almost water insoluble and it was found to be cationic (2.2, pH=7). The LUB950217 enterocin seems to inhibit both gram-positive and gram-negative bacteria growth in vitro, suggesting LUB950217 enterocin can be used in food stuff and animal feed. Furthermore, isolation, cloning, and expression of this peptide may render resistance to fungi and bacteria pathogens in crop plants.

Background and Lactic acid bacteria (LAB) play an important role in human and other organisms life. Some of LABs kill pathogenic and other harmful microorganisms. Enterococcus are, gram-positive, catalase-negative, cocci forming, non-sporogenesis, and facultative anaerobic bacteria. This study was done to identify and isolate possible probiotic bacteria with benefits to human health from Persian oak sap (Quercus brantii var. persica). The aim was to identify beneficial bacteria for plant pathogenic biological control and industrial applications.
LABs were identified using conventional methods, including culture dependent methods and 16S rRNA sequencing method. Antibiogram analysis was performed and the presence of virulence genes, including efaA (endocarditis antigen), as, ace, esp and gelE was examined by PCR.
It was found that out of 285 colonies, 160 (56.1%) were catalase-negative and gram-positive. Results of 16S rRNA sequencing showed that the bacteria isolated bacteria belong to the Enterococcus Faecium species. E. faecium strains of this study were sensitive to a number of clinically important antibiotics such as vancomycin, ampicillin, ciprofloxacin, and nitrofurantoin. Two strains of E. faecium bacteria, one with the ability to produce Co2 from glucose (KX185054) and one with no Co2 production ability (KX185055) were identified. The 16S rRNA sequence of identified strains were deposited in NCBI database. PCR amplification did not amplify virulence genes except efaA (endocarditis antigen).
In this study, two different E. faecium strains were isolated from the oak which can be good candidates for probiotics and biological control.

Enterococci are lactic acid bacteria with ability to produce antimicrobial peptides that called enterocins. Target of present study is research on the presence and antibacterial activity of enterocin A among Enterococcus faecium for their potential as alternative antimicrobial compounds, probiotics to control and reduce the pathogenic bacteria in the gastrointestinal tract and bio-preservatives in food or feed and. In this study occurrence of class II enterocin structural gene (enterocin A) in a target of 42 Enterococcus faecium strains, isolated from gastrointestinal tract of animal have been surveyed. E. faecium identification and occurrence of enterocin A gene was performed by PCR method. Cell-free neutralized supernatant of gene positive strains was used to test bacteriocin production and antimicrobial spectrum of supernatant was assayed by wall diffusion method on the gram-positive and negative indicators bacteria Based on our results, 73.8% of isolated strains had enterocin A gene that they inhibited growth of indicator bacteria such as clinical strain of Pseudomonas aeruginosa, Salmonella enteric PTCC1709, Listeria monocytogenes, Bacillus cereus and Bacillus subtilis. Studied enterocins have growth inhibitory spectrum on Gram-positive and Gram-negative bacteria especially against pathogenic bacteria in the gastrointestinal tract. Therefore, these strains have the potential to explore and use as, alternative antimicrobial compound and bio-preservatives in food or feed or as probiotics.