جستجوی مقالات مرتبط با کلیدواژه « ایمونوگلوبولین e » در نشریات گروه « پزشکی »

The monitoring and management of blood glucose levels are of significant importance in individuals diagnosed with diabetes mellitus. Accordingly, the objective of this study was to examine the potential of a non-invasive approach for the diagnosis and management of diabetes, through the measurement of glucose concentration and salivary IgA, and a comparison with fasting glucose and serum IgA levels in individuals with type 1 and 2 diabetes, in relation to a control group of healthy individuals.

This case-control study was conducted on 76 individuals with diabetes (31 with type 1 diabetes and 45 with type 2 diabetes) and 24 healthy individuals. After obtaining the code of ethics and informed consent, serum and salivary IgA levels as well as fasting glucose, were measured for each participant. The data were analyzed using SPSS version 16 statistical software and the Spearman, Kruskal-Wallis, and Mann-Whitney tests.

The results showed a significant difference among the three groups (type 1 diabetes, type 2 diabetes, and healthy group) based on Fasting Blood Glucose, Hemoglobin A1c, Immunoglobulin A, salivary sugar, and salivary IgA (p<0.001). Additionally, in the case group, parameters such as FBS, HbA1c, and salivary IgA showed a significant correlation with salivary sugar. Conversely, in the control group, only the salivary sugar level demonstrated a significant relationship with serum glucose level. The study's findings indicated that serum and salivary IgA levels were lower and higher, respectively, in the control subjects compared to the diabetic group (p<0.001).

In healthy individuals, salivary sugar can serve as an indicator to determine fasting blood sugar levels. In diabetic patients, salivary sugar can predict of FBS, HbA1c, and salivary IgA levels.

Cytomegalovirus (CMV) stands out as a significant contributor to congenital viral infections, exhibiting a prevalence ranging from 40% to 90%. Cytomegalovirus has different effects on people, such that it appears without symptoms in people with a healthy immune system, but it leads to severe symptoms in infants and those with a weak immune system. To comprehend the disease's societal prevalence, seroepidemiological investigations are imperative. This study aims to ascertain the serum prevalence of cytomegalovirus and explore potential correlations between age, sex, and the prevalence of CMV in patients attending Shahid Motahari Clinic in Shiraz

A cross-sectional descriptive study was undertaken on 2,469 individuals who assessed their cytomegalovirus antibody levels between March 2019 and February 2023 at Shahid Motahari clinic in Shiraz. The ELISA method was employed to evaluate CMV, and the obtained data were analyzed using SPSS version 19 software. Chi-square tests and Fisher's exact test were applied to examine the correlation between positive antibody results and age as well as gender.

Among the 2469 surveyed people, 658 people (26.65%) were men and 1811 people (73.35%) were women. Based on ELISA test, antibody titer was reported positive in 1157 people (46.9%), including 315 men and 842 women, and 1312 people (53.1%) had negative antibody titer. The highest number of positives is related to IgG, and in IgM, the test results are mostly negative. IgM prevalence showed no gender correlation but demonstrated a significant association with patient age. Meanwhile, IgG prevalence exhibited significant relationships with both age and gender

Considering that the city of Shiraz is considered as one of the centers of treatment in the country and a large number of patients from all parts of the country and even neighboring countries come to this city for treatment and especially for the purpose of organ transplantation; also with Considering the 46.9% prevalence of CMV infection in the region, it is recommended to use preventive methods such as vaccine and immunotherapy against CMV infection in patients

 Various factors, including viruses, are involved in developing liver cirrhosis. Although the association of human herpesvirus-8 (HHV-8) with liver cirrhosis remains poorly understood, a significant increase in the titer of antibodies against this virus has been observed in cirrhotic patients. The present study aimed to evaluate the frequency of HHV-8-immunoglobulin G (HHV-8 IgG) in Iranian patients with liver cirrhosis.

In this cross-sectional study, 30 healthy blood donors and 30 patients with liver cirrhosis related to hepatitis B (n=20) and hepatitis C (n=10) viruses from those waiting for liver transplantation were included. The serum seropositivity of HHV-8 IgG antibody was evaluated using the enzyme-linked immunosorbent assay (ELISA) technique.

The seropositivity of HHV-8 IgG antibodies in patients and healthy groups was 6.7% (2 patients) and 3.3% (1 case), respectively, which did not show a significant difference between the two groups (P=0.554). In addition, the infection was not significantly related to the age and gender of the patients (P>0.05).

Considering the low prevalence of HHV-8 infection in Iranian patients with liver cirrhosis, it can be concluded that HHV-8 may not be associated with the incidence and progression of liver cirrhosis. Therefore, HHV-8 monitoring will not raise the concern about the development or worsening of liver disorders.

Multiple sclerosis (MS) is an autoimmune disease that targets the central nervous system. Various studies have shown that several factors influence this disease’s occurrence and prevent its progress. Multiple studies have shown that several factors affect this disease’s occurrence and prevent its progress. Helicobacter pylori can be mentioned among these factors. This study was conducted to determine Helicobacter pylori infection in MS patients and healthy individuals.

This case-control study was conducted on 100 patients with MS (mean age: 36.99±9.87 years) and 100 healthy subjects (mean age: 38.05±11.38 years) in Golestan province, north of Iran during 2021. The case group included eighty relapsing-remitting cases and twenty secondary progressive cases. Blood samples were taken from both groups, and after separating the serum using the ELISA test, anti-Helicobacter pylori antibody (IgG) was detected by determining the antibody titer.

Helicobacter pylori IgG in the MS and control groups was 21% and 44%, respectively (P<0.05). The mean concentration of IgG in the case group was significantly lower than the control group (13.48±10.83 versus 19.78±16.14 AU/ml). The percentage of positive cases of IgG antibody against Helicobacter pylori in the relapsing-remitting and secondary progressive group of patients with MS was determined as 21.2% and 20%, respectively, and the difference between these two groups was not statistically significant.

The history of Helicobacter pylori infection is less than two times in patients with MS compared to healthy people probably indicate the protective role of this bacterium against this disease.

Background & Aims:

Cytokines are a group of proteins that play the main role in inflammatory responses to pathological stimuli such as inflammation and tissue damage. Also, immunoglobulin A is the main class of antibodies in the body that undergoes changes during physical activities. The purpose of this study was to investigate the effect of a period of resistance training with blood flow restriction on the level of interleukin 6 (IL-6), IgA and TNF-α in judokas.

Materials & Methods:

The present research method was interventional (semi-experimental). The statistical population of the present study was male judokas athletes in Tehran. 30 male judokas were randomly divided using available sampling method into 2 groups of training with blood flow restriction as the first group and training without blood flow restriction as the second group. Before the implementation of training programs, blood samples were taken from the subjects, and serum concentrations of IL-6, IgA and TNF-α were measured by ELISA method. Resistance exercise with blood flow restriction was an exercise with 30% intensity of one repetition maximum with cuff pressure (around the proximal area of the arm). Blood flow restriction was considered to be about 120 to 160 mmHg, which depended on the systolic pressure of each individual. Multivariate covariance test was used for inter-group comparison and dependent t-test was used for intra-group comparison at a significance level of P<0.05.

The results of the inter-group comparison showed that IL6 (P=0.023), TNF-α (P<0.001) and IgA (P=0.019) in the two groups of resistance training with blood flow restriction and traditional resistance training were significantly different.
The results of the intra-group comparison showed that IL6 (P < 0.001), TNFα (P = 0.003) and IGA (P = 0.027) in the resistance training group with blood flow restriction in the post-test stage was decreased compared to the pre-test. Also, IL6 (P<0.001) and TNFα (P<0.001) in the traditional resistance training group had a significant decrease in the post-test phase compared to the pre-test.

According to the research results, it can be concluded that a period of traditional resistance training and resistance training along with blood flow restriction can lead to diminishment in TNF-α and IL-6 levels, which shows the improvement of the inflammatory index. Also, resistance training along with flow restriction has led to a decrease in IgA, which indicates weakening of the immune system. According to the findings of this study, in terms of immune system physiology, muscle injuries and inflammations, it is recommended to eliminate intense resistance training programs and develop appropriate training programs such as resistance training with moderate intensity blood flow restriction for athletes.

Intravenous immunoglobulin (IVIG) is one of the safest biological products, which is effective in the treatment of many diseases. This study aimed to evaluate the rational prescription pattern of IVIG based on the approved indications of the Food and Drug Administration (FDA) among teaching hospitals in Birjand, Iran.

This descriptive-analytical study included 300 patients receiving IVIG who were hospitalized in different wards of Razi and Vali-e-Asr hospitals in Birjand during 2016-19. Demographic characteristics, dose, duration of administration, duration of hospitalization, side effects, and other necessary information were extracted from patients' medical records. The collected data were compared with FDA-approved indications and dosages, followed by identifying the rate of rational prescription of the drug. The data were analyzed in SPSS software (version 19) through independent t-test (or Mann-Whitney) and Chi-square test to compare quantitative and qualitative variables in the groups, respectively.

According to the results, half of the patients were male (n=150; 150%). The mean age of the patients receiving IVIG was 28.17±27.01 years. Most of the patients receiving IVIG (30.3%) were hospitalized in the neurology ward. Guillain-Barre syndrome and immune thrombocytopenic purpura accounted for the most prescription of IVIG. In total, 133 patients (44.3%) (95%CI: 38.6-50.1) received the drug according to the FDA on-labeled indication. The dose of IVIG was rationally based on FDA in 33% of the cases (95%CI: 27.7-38.6).

Due to the limited availability and high price of this product, it is recommended to formulate and closely monitor the implementation of rational IVIG consumption in the hospital based on the regional standard protocol, especially with the presence of clinical pharmacists.

The prevalence of asthma and allergic diseases in the world has increased in recent decades, and it is necessary to identify allergens in different regions. The aim of this study was to evaluate the frequency of allergens in patients with atopic and non-atopic asthma in Dazyani Asthma and Allergy Clinic, Gorgan City, Iran in 2020.

This cross-sectional descriptive study was performed on 96 children with asthma aged 1 to 16 years. Diagnosis of asthma was based on the patient's clinical signs, life-saving medications used, activity limitation established, and pulmonary function tests. Accordingly, the definitive diagnosis of asthma was a 15% increase in forced expiratory volume-1 (FEV1) after inhalation of 2 puffs of salbutamol. Children were divided into two groups of atopic and non-atopic based on experiments. In this study, if immunoglobulin E (IgE) levels were high and Prick test was positive, they were in the atopic group, otherwise they were in the non-atopic group. Serum levels of eosinophils and immunoglobulin E were analyzed by type of asthma and severity of asthma.

Among the studied children, 54.17% had atopic and 45.83% non-atopic phenotype. A total of 71.78% of children with asthma were at least allergic to one of the allergens tested. There were also significant relationships between the severity of asthma and being atopic, immunoglobulin E, and eosinophil count.

Asthma is more severe in children with atopic asthma than non-atopic asthma. The mean of immunoglobulin E and eosinophils in atopic asthma is higher than non-atopic. Immunoglobulin E and eosinophil levels are also directly related to the severity of asthma.

Immunoglobulins are a group of proteins that have important role in defense against microorganisms. Immunoglobulins consist of heavy and light chains. In human, immunoglobulin light chain comprises of two isotypes: Kappa (K) and lambda (λ) based on amino acid differences in carboxylic end of their constant region. Marked changes in the K to λ ratio can happen in monoclonal expansion of neoblastic B cells or HIV infection in neonatal periods. Highly sensitive and specific anti-light chain MAbs have clinical importance in the diagnosis and immunotherapy of patients with B-cell immunoproliferative diseases. Thus, precise determination of specific epitopes in light chains is very important. Computational immunology uses the computational data for more accurate diagnosis of diseases. This study describes determination of conformational epitopes in constant region of human immunoglobulin light chain by computational immunology.
The amino acid residue and third structure of reference human immunoglobulin G light chain was found in PDB database. The second immunoglobulin G structure was defined by Phyre 2 software. Conformational epitopes of the immunoglobulin light chain were specified by CEP software.
In this study five conformational epitopes located on constant domain of human immunoglobulin light chain were determined by CEP software. These conformational epitopes were located in 100-214 amino acid sequences of light chains.
In this study a number of conformational epitopes located on constant domain of human immunoglobulin light chain were determined. These epitopes are valuable tools for generating specific monoclonal anti-immunoglobulin light chain antibodies and might have possible implication in production of specific diagnostic kits for human immunoglobulin light chain, monitoring of monoclonal light chain diseases, treatment of related B cell tumors, epitope mapping of immunoglobulin light chain and evolutionary studies

Immunoglobulins are a group of proteins have important role in defense against microorganisms. Human immunoglobulins are divided into five classes: IgA, IgM, IgD, IgE and IgG. Immunoglobulin G (IgG) is the highest abundant antibody in serum and extravascular fluids. The extent of serum IgG is related to severity of several diseases such as infections, so IgG has great diagnostic worth. Accurate measurement of IgG, needs exact and sensitive diagnostic instruments such as human IgG- specific monoclonal antibodies. Moreover, targeting of IgG has been useful in treatment of a number of diseases. According to experimental studies the Fc region of human IgG is highly immunogenic. Immunoinformatic is a division of immunology uses the computational biology for more precise diagnosis of diseases. The aim of this study was determination of conformational epitopes in the fragment of crystallizable (Fc) fragment of human IgG by immunoinformatic.
The amino acid residues and third structure of reference human IgG were found in protein data bank (PDB). Second IgG structure was defined by Phyre2 software (http://www.sbg.bio.ic.ac.uk/~phyre2/). Conformational epitopes of the Fc fragment in human IgG were specified by ElliPro (http://tools.iedb.org/ellipro/) and DiscoTope (http://www.cbs.dtu.dk/services/DiscoTope) softwares.
In this study two conformational epitopes (one in constant heavy chain 2 (CH2) domain and another one common between CH2 and CH3 domains) sited in Fc fragment of human IgG were determined by ElliPro software. Also, two conformational epitopes (Both common between CH2 and CH3 domains) located to Fc fragment of human IgG were determined by DiscoTope software.
In this study a number of conformational epitopes located to Fc fragment of human IgG were determined by two immunoinformatic softwares (ElliPro and DiscoTope). The epitopes recognized by both softwares were situated in CH2, CH3 or both of these domains in the human IgG heavy chain. Thus, it seems that CH2 and CH3 domains of Fc region in human IgG are highly immunogenic. Moreover, ElliPro and DiscoTope softwares can be useful tools for identification of epitopes located to Fc fragment of human IgG.

Current evidence suggests that coenzyme Q10 supplementation can play a role in enhancing immune function. Relatively, the aim of this study was to investigate the effect of 14-day supplementation of coenzyme Q10 on humoral immune response to intense exercise similar to soccer.
In this quasi-experimental study, 24 soccer players selected with a convenience sampling method and randomly divided into three equal groups (n=8): control, exercise and exercise with supplements Q10. Exercise includes three sessions of 90 minutes soccer specific training and supplementation includes intake of 5 mg/kg Coenzyme Q10 per day for two weeks. Blood samples within 24 hours before and after the intervention were collected from subjects.
The results showed that serum levels of cortisol and the percentage of Neutrophil in exercise group were significantly increased (P=0.001) while levels of IgA (P=0.008) and IgG (P=0.019) significantly decreased. However these changes in exercise and Q10 supplement group was not significant.
short-term supplementation of Coenzyme Q10 with the adjustment of immunoglobulin A and cortisol during strenuous exercise can improve the humoral immune function in soccer players

Physical activity and some antioxidants, such as vitamin C, can affect the immune system. This The current research attempts to determine the immune system reaction following consumption of vitamin C after an aerobic physical activity.
Subjects and In the present experimental work, 24 females aged 32 y were selected by random sampling and divided into two 12-subjects groups, first group (vitamin C) and the second group (placebo). In the pre-test, the serum baseline measurements for IgG and IgA were made for both groups. Then, 500 mg of vitamin C was given to the experimental group once daily for 5 days. After five days, blood samples were taken from both groups. Finally, on the 6th day immediately blood sampling was performed after aerobic physical exercise (Bruce test). And then was taken to the lab by immunological examination after analyzing the data, the required information was collected.
The results showed that after aerobic activity level of IgG and IgA were significantly higher (P The consumption of vitamin C following aerobic exercise has an effect on the levels of A and G immunoglobulin’s and serum cortisol levels, and the repressive effects of the exercise-induced immune system are somewhat reduced.

Introduction & Autoimmune cytopenic purpura (ITP) is a kind of disease accompanied with thrombocytopenia and platelet damage. The first line treatment is corticosteroid therapy, but always it's not effective. The aim of this study is find out the therapeutic value of splenctomy in these patients’s who that refers to speciality Immam Reza hospital between 2005-2015.
In this study 88 patients with ITP who had undergone surgery for splenectomy were evaluated. We used a retrospective and clinical review method. The related data of gender, clinical signs, history of blood transfusion and treatment before splenectomy and patient condition after splenectomy are extracted and analyzed after codifying in SPSS 21 software and descriptive statistic.
The average age of the patients and those who had done splenectomzed were 39 years. The ratio of man to woman was equal. The results show that there is meaningful relationship between response to splenectomy and low age (P = 0.002) and also there is a meaningful relationship between using IVIG before splenectomy and rise platelet number after splenectomy (P = 0.004).
Splenectomy, specialty using IVIG before that is good treatment for ITP.

Immunoglobulin E (IgE) is a key player in allergy diseases and immune responses against parasites. Epidemiological studies indicated that there is an inverse association between plasma IgE levels as it been seen in allergic patients and the risk of cancer development. The aim of this study was to investigate the plasma IgE levels in breast cancer and patients with chronic lymphocytic leukemia than healthy subjects.
In this case-control study, twenty patients with CLL also twenty five patients with breast cancer as the cases groups and twenty age-matched healthy subjects as control group were evaluated. Plasma IgE levels in both groups were measured by ELISA. Data from both groups were analyzed and compared by kolmogorov-smirnov test and paired t-test with SPSS version 16.
The IgE levels were 9.045±4.261 IU/ml in CLL patients and 37.19±12.21 IU/ml in patients with breast cancer and 40.73±16.18 IU/ml in healthy subjects. Our data showed that plasma IgE levels in CLL patients group were significantly less than controls group (p It seems that there is an inverse correlation between serum IgE levels and the risk of developing CLL. While there is no correlation between the IgE level and the risk of developing breast cancer. IgE levels can be used as a biological marker in chronic lymphocytic leukemia development.

Intravenous immunoglobulin (IVIG) as an alternative to plasma proteins (IgG), In patients with low or Immunodeficiency prescribed. Enough IVIG recommended to patients with immune deficiency Immunoglobulins in order to maintain levels of antibody to prevent infection and provide a passive safety. The purpose of this paper is to acquaint the use of Intravenous immunoglobulin in different conditions in children.

This study provides an overview of library resources and databases (Pub med, Medline, Google, Sid, Magiran, …) with the literature review from 2010 to 2014 with keywords (Children, Immunocompromised, Intravenous immunoglobulin) has been developed.

In recent years, intravenous immunoglobulin in the treatment of children as Hematologic disorders such as idiopathic thrombocytopenic purpura, autoimmune deficiency of Factor VIII, red cell aplasia associated with Parvovirus nephropathy immunoglobulin A, membranous glomerulonephritis, lupus, juvenile rheumatoid arthritis and Gillian Barre is used. It often appears during injection and setting the speed and amount of injection can be controlled. Headache is the most common complication. Chills, dizziness, fever, itching, are possible side effects of this drug.

Overall immunoglobulin therapy is a tolerable intravenous in pediatric patients with antibody deficiency However, all patients receiving IVIG in the treatment of symptoms should be monitored, risks, side effects of IVIG, management and use of IVIG familiar place.

Planting Spartium junceum L. has increased immensely in urban areas due to its aromatic petals. Since the petals and stamens in aromatic plants are of important herbal allergenic agents, the aim of this study was to investigate the allergenicity of petal and stamen at old ontogenical staged Spartium junceum L. in Guinea pig. In this experimental study, 21 male Hartley Guinea pigs were randomly divided into three groups including 7 animals. To the first group buffered phosphate saline, second group old petal extract and the third group petal and stamen extract were injected. The extracts were prepared with 16% concentration. The injections were continued within 4 weeks, once per week intraperitoneally and in the fifth week subcutaneous injection was performed. A week after the last injection blood sampling was done from the heart of animals and the number of Eosinophils, Immunoglobulin E and blood sugar levels were measured. To data analysis SPSS 16 software, ANOVA and dependent T tests were used. In skin‌‌‌‌ test wheal diameter in both groups treated with Spartium junceum L. significantly increased compared to control group (p<0.001). Blood sugar in groups treated with petal and stamen showed significant increase in comparison with control group (p<0.05). In electrophoretic profiles 3 protein bands was observed in the range of 46 to 85kD in both treated groups which these bands were much more colorful in petal and stamen group. Allergenicity of petal with stamen of Spartium junceum L. at old ontogenical stage is more than petal.

Lead as an environmental pollutant can damage the immune system. This study was done to determine the effect of moderate-intensity exercise and Curcumin supplement on serum level of immunoglobulin A in rats exposed to lead acetate. In this experimental study, 46 male rats were allocated into six groups including; Control (C), vehicle (V), lead (L), lead and exercise (LE), lead and curcumin (LC), Lead+exercise+curcumin (LEC). Animals in training groups ran on treadmill for 8 weeks (25- 64 minutes per daily, 15-22 meter/minute and 0% grade). Animals in Lead, LE, LC and LEC groups were received lead acetate (20 mg/kg/bw) and vehicle animals were received ethyl oleat (30 mg/kg/bw) Intraperitoneally for 3 days per week for 8 weeks. Rats in curcumin and LEC groups were received Curcumin (30mg/kg/bw), for 3 day per week for 8 weeks. IgA level were measured by single radial immuno diffusion method. Lead acetate significantly increased MDA levels at P<0.05 in animals. IgA level was 0.2, 0.41 and 0.47 mg/dL in Lead, LE and LEC groups (P<0.05). The simultaneous use of endurance exercise training and curcumin due to increased IgA activity has beneficial effects against lead poisoning.

Probiotics are live microorganisms, which if administered in adequate amounts,have beneficial effects on host health. Probioticsreduces the risk of developing diseases in the consumers through prevention of the immunosuppressive effects. The present study was performed with the purpose of comparing the effect of probiotic supplementation on the immune response in male athletes, before and after exhaustive exercise. In a double-blind study, 16 male athlete volunteers aged between 19-25 years were assigned into two groups of experimental and placebo. Subjects of the experimental and placebo respectively tooktwo capsules of probiotic or placebo daily for 30 days. Blood samples of the participants were collected at the beginning and on the 30th day, and the numbers of lymphocytes, monocytes, granulocytes, serum CRP, and serum IgA were measured. Data were analyzed using repeated measurement method and independent t-test. Consumption of probiotics supplement in the rest levels in the intragroup and intergroup comparison,only showed significant difference (p<0.05) in the monocytes levels compared to base line level.Also, after the exhaustive exercise, a statistically significant difference was observed in the levels of monocytes, granulocytes, and lymphocytes compared to the baseline levels.In the comparison between the probiotic and placebo groups, only the monocytes level were significantly different (p<0.05). Other variables had nosignificant differences. The results of this study showed that probiotic consumption following exhaustive exercise can cause increase infactors of lymphocyte, monocyte, and geranolocyte, thereforeit seems that probiotics play a role in the development of immune system.