جستجوی مقالات مرتبط با کلیدواژه "اینتگرون ها" در نشریات گروه "پزشکی"

Transfer of antibiotic resistance genes by integrons is the main cause ofdrug-resistant bacteria. This study aims to evaluate the prevalence of class I, II, and III integrons amonguropathogenic Escherichia Coli (UPEC) strains isolated from patients with Urinary tract infection (UTI).

This cross-sectional study was carried out on 50 UPEC strains isolated from patientswith UTI referred to hospitals in Shiraz, Iran in 2020. Antibiotic susceptibility pattern was evaluated bythe disk diffusion susceptibility test. Then, the prevalence of class 1 to 3 integrons in the isolates wasinvestigated by the polymerase chain reaction test. Data were statistically analyzed in SPSS softwareusing chi-square test. P≤0.05 was statistically significant.

42% of isolates had multi-drug resistance. The highest antibiotic resistance and sensitivitywere related to trimethoprim/sulfamethoxazole (52%) and gentamicin (90%), respectively. Therewas a significant relationship between the presence of class I integron and resistance to amikacin andciprofloxacin, between the presence of class II integron and resistance to gentamicin, and between thepresence of class III integron and resistance to trimethoprim/sulfamethoxazole and nalidixic acid (P<0.05).

There is a significant association between the presence of class I, II and III integrons andantibiotic resistance in UPEC strains isolated from patients with UTI. Infection control measures andsuitable treatment methods are needed for preventing the spread of these isolates in the hospitals andhealth centers in Shiraz city.

In recent years, the role of integrons has been identified in the transfer of antibiotic resistance genes. The aim of this study was to determine the antibiotic resistance pattern and to identify class I and II integrons and associated gene cassettes in clinical isolates of methicillin-resistant Staphylococcus aureus.
In this descriptive cross-sectional study, 100 isolates of S. aureus were isolated from clinical samples in 2015. After cultivation, the isolates were verified by standard biochemical tests. Then, PCR test was used on nuc gene for final confirmation of the isolates and presence of methicillin-resistance gene. Then, methicillin-resistant isolates were tested for susceptibility to 9 antibiotics. Finally, identification of Class I and II integrons genes and associated gene cassettes was performed on mecA gene using PCR method. Data analysis was carried out using Chi square test.
Forty-one out of 100 S. aureus isolates carried mecA gene. The most frequent antibiotic resistance was for cefoxitin and tetracycline (41 and 36 isolates, respectively) and the lowest resistance was reported for vancomycin (0). In this study, The prevalence of 38 isolates (92.68%) and 3 isolates (7.31%) produced class I and II integrons. One type of gene cassette was identified in these isolates, which was related to aadA1 gene cassette.
The results of the current study were indicative of high prevalence of antibiotic resistance and class I integrons in S. aureus isolates carrying mecA gene.

Multi-drug resistant (MDR) Enterobacter spp. containing class 1 integrons are considered as one of the major concerns of clinicians and infection control specialists. The aim of this study was to determine the association between class 1 integrons and the multidrug resistance pattern among Enterobacter spp. In this analytical study، antimicrobial susceptibility pattern were determined among 137 isolates of Enterobacter from Qazvin and Tehran hospitals during May 2011-September 2012. Then all isolates were screened for class 1 integrons using PCR assay. Data were analyzed using chi-square test and Fisher''s exact test. The MDR pattern was found in 83 isolates (61%) and class 1 integrons were detected in 52 (63%) of these isolates. There was a significant association between the presence of class 1 integrons and the MDR pattern. With regards to the results، use of proper equipment for infection control and appropriate treatment strategies are necessary.