جستجوی مقالات مرتبط با کلیدواژه "تکرار taaaa" در نشریات گروه "پزشکی"

Genetic alternation in p53 gene is associated with tumorgenesis, specially breast, colon and lung tumors. To our knowledge, there has been no study on the relationship between TAAAA repeat in the first intron of p53 gene and cancer risk. The aim of this study was to investigate polymorphism of TAAAA in the first intron of p53 gene among colorectal cancer patients and healthy individuals and its relation to risk of colorectal cancer.
In this research, 151 blood samples of patients with colorectal cancer and 180 healthy samples were studied. After DNA extraction from peripheral blood samples by salting out method and amplification of desired sequence, the number of TAAAA repeats was determined by Polyacrylamide gel electrophoresis and direct sequencing.
In this study, five different length of TAAAA repeat in the range of 6-10 and 13 allele combinations (genotypes) were observed among patients and controls. The most frequent allele in both patients and controls was the 8-TAAAA repeat. Results of homozygous genotypes equal or below 8 are higher among the controlled than the case subjects. In the contrary, number of genotypes equal or higher than 9 (9.10, 9.9) was higher among the patients. Due to the insufficient number of samples, the statistical analysis didnot not show a meaningful relationship. There is no meaningful relationship between genotype, metastasis and age.
Our study did not show a strong association between the TAAAA repeat polymorphism in p53 gene and the risk of colorectal cancer.

Prostate cancer has a high degree of heterogeneity in pathology and clinical appearance. In response to stress, P53 plays an important role in preventing cancer development. To our knowledge, this is the first report about polymorphic TAAAA repeat of the P53 gene and its relation to prostate cancer risk. The purpose of this study was to investigate polymorphism of TAAAA in the first intron of p53 gene among prostate cancer patients and healthy individuals and its relation to risk of prostate cancer.
A total of 306 peripheral blood samples consisting of 156 from patients with prostate cancer and 150 from healthy control individuals were included in the study. DNA was extracted from blood using salting-out method. TAAAA repeat sequences were amplified by PCR technique and the length of products was determined by polyacrylamide gel and direct sequencing.
Findings: Based on our results, 5 alleles and 12 different genotypes for P53 TAAAA repeat polymorphism were observed. The most common allele in both patients and controls was the allele 8/8. Men who carry 9/9 or 10/10 genotypes of p53 gene are at significantly higher risk of prostate cancer. The allelic length of p53 polymorphisms had no significant effect on age of onset, inheritance as well as metastasis.
Our study showed a strong association between the TAAAA repeat polymorphism in P53 gene and risk of prostate cancer.

Genetic alternation in p53 gene is associated with tumorgenesis, especially in breast, colon and lung tumors. Somatic mutation of the p53 gene is the most common genetic alteration seen in human cancers. To our knowledge, before this research, there was no study on the relationship of TAAAA repeat in the first intron of p53 gene and cancer risk. The purpose of this study was to investigate the polymorphism of TAAAA in the first intron of p53 gene among patients with breast cancer and healthy individuals and its relation to risk of breast cancer.

Peripheral blood samples were collected from 200 women with breast cancer and 200 healthy women. After DNA extraction from peripheral blood samples via salting out method and amplification of desired sequence via polymerase chain reaction (PCR), the number of TAAAA repeats was determined using polyacrylamide gel electrophoresis and direct sequencing.

Five different length of TAAAA repeat in the range of 6-10 and 11 allele combinations (genotypes) were observed among patients and controls. The most frequent allele in both patients and controls was the 8-TAAAA repeat. Women who were homozygous for (TAAAA)7 or heterozygous for (TAAAA)6 were at higher risk of developing breast cancer. The allelic length of p53 polymorphisms had no significant effect on the metastasis, the expression of estrogen receptors, progesterone receptors and ErbB2 (HER2) and age of the onset.

Our study shows strong association between the TAAAA repeat polymorphism in p53 gene and risk of breast cancer.