جستجوی مقالات مرتبط با کلیدواژه « شقایق کوهی » در نشریات گروه « پزشکی »

Glioblastoma tumors are the most invasive brain tumors with the origin of neural tissue in the brain. Because of the low success rate of treatment for this type of tumor, the need for a search for new therapies is justified. Glacium flavum is widely used in the pharmaceutical industry due to its richness in alkaloids. In this study, the toxic effects of hydroalcoholic extract of Glacium flavum on glioblastoma cell line (C118) and neuronal neurosphere (NCs) were investigated.
First, The extract of Glacium flavum has been distilled. Neuronal precursor cells from cortex of 17-day-old pregnant mouse fetus were extracted by enzymatic digestion. Then, at concentrations of 100, 300, 500 and 1000 µg/ml of Glacium flavum, the cells were treated at 24 and 72 hours intervals. After treatment, the viability of the cells was evaluated by MTT method and morphology of the cells by DAPI staining.
The experimented cells were morphologically modified after treatment with Ic50 concentration of Glacium flavum. The evaluation of cell viability by MTT showed that Glacium flavum significantly reduced the viability of C118 cells as well as neurosphere (NCs) in both 72 and 24 hours intervals. The severity of Glacium flavum cytotoxicity on glioblastoma cells was higher than neurospheres and showed a significant difference (P <0.05). Also, the quantitative results of the cell viability indicated that concentrations of 300 and 500 µg/ml of the extract of Glacium flavum has been as the IC50 concentration for C118 cells and NCs respectively.

Finally, it can be concluded that natural products such as Glacium flavum with its antioxidant and anti-inflammatory pharmacological and biochemical properties and as regards it has less site effects on non-cancerous cells so, can be effective in treating glioblastoma tumors.

Thyroid dysfunction is one of the controversial complications of diabetes. Given the hypoglycemic properties of Yellow Horne Poppy (YHP), this study was conducted to evaluate the effect of oral administration of aqua-ethanolic extract of YHP and glibenclamide on serum level of T3 and T4 hormones in alloxan diabetic rats compared to healthy rats. In this experimental study, 56 male Wistar rats were randomly divided into seven groups of 8, including healthy control, healthy treated with 250 and 500 mg/kg of YHP extract, diabetic control, diabetic treated with 250 and 500mg/kg of YPH extract and diabetic treated with 5μg/kg of glibenclamide. Diabetes was induced by intraperitoneal injection of a single dose of alloxan. After 30 days of treatment, blood samples were collected and the concentration of thyroid hormones T3 and T4 were measured. Finally, the data were analyzed using one-way ANOVA. The serum concentrations of thyroid hormones were significantly lower in the diabetic control group than those in the control group (P<0.001). The mean concentration of T3 was significantly higher in the diabetic+250 extract than that in the diabetic control group (P<0.001). Also, the mean concentration of T4 was significantly higher in the diabetic+250 extract than that in the diabetic+drug group (P=0.04). YPH extract can effectively improve T3 and T4 serum levels in diabetic rats. Further studies are required to determine the active ingredient responsible for this therapeutic effect, such as Glaucin.

Yellow Horned Poppy (YHP) is known as anti-diabetic plant in folk medicine in the southern regions of Iran. This study was conducted to compare the effects of oral administration of this plant and Glibenclamide on serum level of Blood Urea Nitrogen (BUN), Creatinine and Bilirubin (total and direct) in Alloxan induced diabetic rats. in this experimental study, Forty adult male Wistar rats were randomly divided into five equal groups: healthy control, diabetic control, diabetic treated with 250 mg/kg Body Weight(BW)/day of extract, diabetic treated with 500 mg/kg BW/day of the extract and diabetic treated with 5μg/kg BW/day of Glibenclamide. Diabetes induced in groups 2 to 5 by IP injection of Alloxan at a dose of 120 mg/kg BW. After thirty days, the animals were anesthetized and blood samples were collected from the heart. Creatinine, BUN, total and direct bilirubin were measured enzymatically. The results were analyzed by one-way ANOVA and Tukey's post hoc test. there were significant different between means of 250 treated group and diabetic control in all four factors (P<0.001). Also, the difference of means were not significant between 500 treated group and healthy control in BUN, creatinine, total and direct bilirubin (P>0.05), this reduction for the drug treated group was limited to creatinine and total bilirubin. It can be concluded that oral administration of the extract at both doses may possibly modulate the renal and hepatic clearance activity of diabetic rats.

Since Yellow Horned Poppy (Glaucium flavum) has antioxidative effects, this study was conducted to determine its effect on some antioxidative enzymes of liver and kidney in Alloxan induced diabetic rats. In this experimental study, 40 adult male Wistar rats were randomly divided into 5 groups of 8 including: control, Diabetic, Diabetic treated with 250 and 500 mg/kg of the extract, and Diabetic treated with 5 µg/kg of Glibenclamide. Oral administration of the extract continued for one month after diabetes induction. At the end, the activity levels of Catalase (CAT), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPX) were determined. Data were analyzed by one-way ANOVA. In the liver and kidney tissues, CAT and GPX activity in the Diabetic+250 group had a significant difference with the Diabetic group (p=0.001). Regarding the results, Yellow Horned Poppy extract can increase the activity of liver and kidney oxidoreductase enzymes in diabetic rats.

Yellow horned poppy is known as an antidiabetic drug in Iran folk medicine. This study was conducted to compare the effect of yellow horned poppy and glibenclamide on blood glucose concentration, blood lipid profile and weight of alloxan-induced diabetic rats. 40 male rats were randomly divided into five groups of eight: 1)healthy control 2)healthy treated with 500 mg/kg of body weight (BW)/day of the extract 3)diabetic control 4)diabetic treated with 500 mg/kg of BW/day of the extract 5)diabetic treated with 5µg/kg of BW/day of glibenclamide. Diabetes induced in the 3rd,4th and 5th groups  by intraperitoneal injection of 120 mg/kg BW of alloxan. The weight of rats was measured after 1, 14 and 28 days. After a month, animals were euthanized and blood samples collected to measure serum levels of fasting blood glucose, triglyceride, cholesterol and lipoproteins including: HLD-C and LDL-C by an auto-analyzer. Data analysis was performed by repeated measure Anova, one way Anova and Tukey test. We found a significant decrease in glucose concentration in the diabetic+extract group compared to the diabetic control (P<0.001), this decrease was significantly lower than that in the diabetic+drug group (P=0.03). Triglyceride, cholesterol and LDL concentrations in the diabetic+extract group showed a significant decrease compared to those in the diabetic control group (P<0.001). Significant increase in HDL concentration was observed in the diabetic+extract group compared to that in the diabetic control group (P<0.001). The present study showed antidiabetic effect of yellow horned poppy in the rats which is comparable to the effect of glibenclamide.

Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Horned Poppy on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan induced diabetic rats.

In this experimental study, 32 male rats were randomly divided into four groups of eight control, diabetic control, diabetic treated by a dose of 500mg/kg body(diabetic+extract) diabetic treated with Glibenclamide by 5μg/kg(diabetic+drug). Diabetes were induced by single injection of 120mg/kg of Alloxan. After a month of Oral administration, cardiac and cephalic activity of SOD, CAT and GPX were measured and were analyzed.

Cardiac activity of all three enzymes in the group diabetic+extract were significantly higher than diabetic control (P<0.001). Cephalic activity of SOD had a significant different in comparison of diabetic+extract(8.79±1.4) and diabetic+drug(6.77±1.7) (P=0.03). As the same, CAT activity in diabetic+extract(4/64±1.2) were significantly higher than diabetic+drug(3.83±1.5). And GPX activity in diabetic+extract(4.23±0.7) were significantly higher than diabetic+drug(3.64±0.4) too (P=0.03)

The present study declared that yellow horned poppy`s extract can promote the main enzymatic mechanisms against diabetic induced oxidative stress in heart and brain which it was more evident in brain.