جستجوی مقالات مرتبط با کلیدواژه "لوسمی حاد" در نشریات گروه "پزشکی"

Prunus armeniaca is one member of the Rosaceae family. Antitumor activity of this family on the colon, prostate, bladder, cervix, lung and breast cancers has been proven in previous research, which is attributed to a natural compound called amygdalin. Therefore, in this study, we investigated the effect of apricot kernel extract on acute leukemia that has not been studied before.

In this experimental study, Aqueous, ethyl acetate, and hydro alcoholic of the Armeniacae semen extracts were prepared. The NALM-6 and KG-1 cell lines were treated with different doses of extracts; cell viability was investigated with MTT test and using double staining fluorescent, cell apoptosis was detected. Cell cycle progression was analyzed, using a BD Cycle TEST PLUS DNA Kit.

The results showed that different extracts of Prunus armeniaca induced dose-dependent inhibition in both Kg1 and Nalm6 cell lines. According to the results of MTT test, the strongest IC50 in Kg1 and Nalm6 cell lines was related to the 48h effect of ethyl acetate extract of Prunus armeniaca. Fluorescent microscopy analysis showed apoptosis. The ethyl acetate extract application in both cell lines increased the number of cells in the G0 / G1 phase and decreased the number of cells in the G2 / M phase.

It seems that ethylacetat extract of Prunus armeniaca can reduce the proliferation of Kg1 and Nalm6 cell lines, probably by regulates cell cycle progression.

Childhood cansers are the second leading cause of death among children. Since the incidence and prevalence of malignancies are different in different regions and there are several cultural and environmental factors involved, the aim of this study was investigating epidemiology of common malignancies in children in Hamedan Province during the years of 1386-1395.
In this cross-sectional study, the population study was the children with malignancies referred to Besat's Hospital in Hamadan Province. The collected data were analyzed using SPSS 20 software. Descriptive indices and Chi-square test were used in this study.
All available records showed that the malignancy was more prevalent in boys in freqency (57/7%). Most acute lymphoblastic leukemia (ALL) patients were 1-9 years, most acute myeloid leukemia (AML) patients were older than 9 years and most other malignancies were 1-4 years and over 9 years. The number of blood platelets (PLT) and the white blood cells (WBC) of the majority of patients in other malignancies, respectively, were greater than 100,000 and less than 50,000. For acute lymphoblastic leukemia and acute myeloid leukemia patients, the number of PLT and WBC were less than 100,000 and less than 50,000, respectively. The most clinical symptoms for acute lymphoblastic leukemia patients were fever and pale, fever and bleeding for acute myeloid leukemia AML patients, and finally masses and fever for patients with other malignancies.
Although the results of the family cancer were consistent with expected results concluded of similar studies, but the incidence of other malignancies in females was different (51/1%) with the results of some other studies.

Acute lymphoblastic leukemia is a disorder caused by the proliferation of malignant immature lymphoid cells. GRAF (GTPase regulator associated with focal adhesion kinase) which acts as a tumor suppressor gene is disabled in hematologic malignancies due to genetic and epigenetic abnormalities. In this study, GRAF gene expression was inspected in patients with B-ALL.
Peripheral blood samples from 60 patients with B-ALL and 30 healthy controls were collected. DNA was extracted and cDNA was synthesized. GRAF gene expression was measured using Real-Time PCR and the relationship between GRAF expression and clinical and laboratory findings were investigated.
GRAF gene expression was significantly decreased in patients compared to the control group (0.87).
The number of patients who had a reduction in their GRAF gene expression was 73.4% (44 of 60 patients). GRAF expression had no significant difference in the FAB morphological subtypes.
Results of this study showed that GRAF gene expression is decreased in acute lymphoblastic leukemia. The reduction in GRAF gene expression confirms its tumor suppressive role in this leukemia.

The integration of T (8; 21), or AML1-ETO, is the most common translocation in acute myeloid leukemia at both ages of children and adults. The occurrence of this genetic integration is effective in the future of the patient's treatment and has a prognostic role. In the Iranian population of acute leukemia, this genetic integration is unclear and its prevalence is unclear. Therefore, the present study aimed to investigate the integration of AML1 / ETO gene in children with leukemia referring to Imam Khomeini Hospital in Tehran.
In this cross-sectional study, 58 children with acute myeloid leukemia with different morphology during the year 95 were evaluated. After peripheral blood supply, the mononuclear cells of the patients were immediately removed. Also, the peripheral blood lymphoma was prescribed to confirm leukemia. Single-core cells were extracted from patients with complete RNA. Using PCR molecular techniques, Nested PCR and Real-Time PCR were used to evaluate the integration.
The results of this study showed that the age range of the children was between 4-5 years old. The sex ratio was 53.5% female and 46.5% male. 80% of the subjects had this genetic integration, as well as their plasma mRNA was significant. In the peripheral blood test, hemoglobin concentration was 9.6 g / dl and the average white blood cell count was 70500 / μl.
Considering the frequency of integration of this gene translocation in the Iranian population studied, it seems that the use of this integration is useful for determining

Background and Acute leukemia is a malignancy with a rapidly progressive course of the lymphoid or myeloid cells. A prognostic factor for its survival and treatment is of particular importance in medical research. Therefore, this study aimed at applying competing risk model to compare clinical and pathologic factors in pediatric acute leukemia. A historical cohort analysis was performed on survival data of 97 children with acute leukemia collected from Pediatric Oncology Department in Booali Sina Hospital in Sari from 2006 to 2014. We recorded data including age at diagnosis, type of leukemia, sex, white blood cell count, and platelet count. Statistical analysis using competing risk model, Cox regression model and STATS software were performed. The median follow-up period was 14.83 mounts during which 27 patients (27.83%) (17 ALL cases and 10 AML cases) passed away. In competing risk model significant relationship was found between type of leukemia, hemoglobin count and white blood cell count with survival time. In order to identify the prognostic risk factors on the survival of patients with acute leukemia, in presence of competing risks, using the competing risk regression model was found more efficient compared to cox regression model.

Semi-Markov multi-state models are very important to describe regression and progression in chronic diseases and cancers. Purpose of this research was to determine the prognostic factors for survival after acute leukemia using multi-state models.

In this descriptive longitudinal research, a total of 507 acute leukemia patients (206 acute lymphocyte leukemia, (ALL), 301 acute myeloid leukemia (AML)) at Shariati Hospital, Tehran, Iran were selected. The median of follow up time was 1.5 year. A multi-state model with four states, (state 1: bone marrow transplantation, state 2: chronic graft verses host disease( cGvHD), state 3: platelet recovery, state 4: death, (absorb state) ) were defined. Weibull distribution was considered as transition time between states. Data was analyzed using R Ver 2.13 software.

With increasing sojourn time in state 1, hazard of cGVHD increased, the effects of some covariates were not constant during disease time for example after platelet recovery, death hazard of acute lymphoblastic leukemia patients was 2.15 times of acute myeloid leukemia patients. There was a negative correlation between sojourn time in state 1 and state 2(r = -0.17, P<0.001).

Semi-Markov multi-state models are useful models to accommodate multiple events and time dependent covariates.

Acute leukemia is one of the main causes of cancer in the world. Now a days using natural materials as source of anticancer drugs is more recommended. HESA-A is a drug of herbal-marine origin (patented by Iranian researcher). HESA-A is composed of 50% inorganic substance، 45% organic substance (aminoenthraquinone) and 5% water. In this study effects of HESA-A، on NB4 cell line (Acute promyelocytic leukemia cells) was evaluated. HESA-A was prepared in normal saline as a stock solution (80 mg/ml, PH=7. 4), and then was sterilized. After culturing and proliferation of NB4 cell line, the cells were treated by doses of 1, 2, 4 and 8 mg/ml of HESA-A. Respectively after 72h, the percentage of viable and dead cells were counted by using Trypan blue staining in Neubanr hemocytometer. Then by MTTassay, the percentage of cell survival were determined by ELISA reader in 570nm. Finally the effects of HESA-A on apoptosis were evaluated by flocytometery. This invitro study shows that HESA-A has a cytotoxcic and antiprolifrative effects against NB4 cell line (Dose dependent). IC50 dose was 5mg/ml. HESA-A can result in apoptosis in 50% of the cells. Although the mechanism of HESA-A cytotoxicity action is not known, yet this study shows that this drug may cause apoptosis of cells by dose dependent method.

In this study we aimed to determine factors related to the etiology of cancer in children. We investigated this issue in a case control study of 300 patients, aged 2±14 years, with acute leukemia, and 300 controls, individually matched to cases for age and sex, and place of residence. Children were enrolled after selection by a continuous sampling method. Information regarding breast-feeding was obtained through face to face interviews with mothers. Breastfeeding for durations less than one month increased the risk for childhood acute leukemia. Also, results showed that children 18-13 months and 24-19 months were breast fed, respectively, 2.45 and 3.54 times less than had been breastfed children under 1 year were diagnosed with acute leukemia. (P<0.05). Higher number of children (OR=1.42, CI 1.03-1.96) and low family income (OR=0.6, CI 0.38-1.01) increased the risk for childhood acute leukemia (p<0.05). Results of this study indicate that childhood acute leukemia may be associated with breast-feeding, economic status, and number of children. Further cohort studies on factors causing acute Leukemia in children are recommended.

There are some reports about probable association between acute leukemia and special blood groups. Frequency of ABO and RH blood group is different in each population. This study was done to determine frequency of ABO and Rh blood groups in patient with acute leukemia and healthy population. This case - control study was done on 214 patients (132 males, 82 females) that suffering from acute leukemia as case group and 117026 individuals healthy population who were voluntary blood donors as controls in Mashhad, Iran during 2001-07. Age, sex, ABO blood group and RH blood group were collected from hospital files for each cases. Furthermore the results of ABO and RH blood groups of controls were collected. Data were analyzed with Chi-Square, fisher test and Odd’s ratio. The frequency of A, B, AB, O and RH blood groups in cases were 23.8%, 32.8%, 7.0%, 36.4% and 90.7%, respectively. The frequency of A, B, AB, O and RH blood groups in controls were 29.8%, 27.4%, 8.9%, 33.9% and 88.3%, respectively. Odds Ratio test didn’t show association of ABO and RH blood groups with acute leukemia. Odds Ratio test showed association of B blood group with acute leukemia in females (OR=0.571, 95%CI: 0.358-.0908, P=0.021). This study showed although there was not any association between ABO and RH blood groups and acute leukemia but probably females with B blood group are more susciptible toward acute lymphoblastic leukemia.

FLT3-gene mutations cause leukemic cells to proliferate uncontrollably and leads to a poor prognosis. The aim of this study was to explore appropriate at diagnostic molecular tests and to screen mutations that occur in patients with acute leukemia. In this basic study, 91 children with acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) were investigated for FLT3-gene mutations, including ITD mutation (Internal Tandem Duplication) and the point mutation that is coded by exon 17. ITD mutation in FLT3 receptor was analyzed by PCR (Polymerase chain reaction) in 11, 12 exons and 11 intron using designed primers. For analysis of point mutation of Exon 17 in FLT3 receptor gene, the genomic DNA of patient was amplified using the PCR. Resulted PCR products were studied by ECORV enzyme and restriction length polymorphism (RFLP). In cases of positive ITD, the sequencing method was applied. Of 91 acute leukemia patients, ITD mutation was observed in 7 cases. Two of 91 patients had point mutation of D835. Distribution of ITD and point mutation of D835 mutation was not identical in FAB subtypes. FLT3-gene mutations are prevalent mutation in children with acute leukemia. So, it can be decided about the treatment after molecular diagnosis of this mutaions, independent of FAB classification and before the treatment get started.

The process of acute leukemia and adverse effects of chemotherapy are long lasting and substantially reduce the quality of life in patients. So, self-care programs should be developed to maintain independence and sense of well-being in patients. The aim of this study was to determine the effects of a self-care educational program on quality of life (QOL) of patients with acute leukemia. A quasi-experimental study was conducted on 70 patients in Tabriz Hematology and Oncology Center. The patients were randomly divided in two groups of case and control. The Quality of Life /Cancer Survivors (QOL-CS) questionnaire was used to measure the quality of life. This questionnaire consists of four (physical, psychosocial, social and spiritual) domains. The patients completed the questionnaires two weeks after completion of first chemotherapy program. A self-care educational program was conducted and booklets were given to the patients of study group. All patients were followed for 4 months. The quality of life of both groups was again measured and compared. There was no significant difference between two groups in the items such as age, sex, level of education, income and occupation (P>0.05). There was no significant difference between QOL mean scores of both groups before the intervention, however the QOL mean in patient group (221.63 ± 37.36) improved significantly compared to control group (177.20 ± 21.82) (P= 0.000). Also, the overall QOL decreased in control group (P=0.000). Our results confirmed the positive effects of educational self-care programs on the QOL of patient with acute leukemia. Extensive educational and self-care programs along with long-term follow up are suggested.