جستجوی مقالات مرتبط با کلیدواژه "نانو ساختارها" در نشریات گروه "پزشکی"

Annually, a large part of the world population is infected with trichomoniasis. On the other hand, the common medicines used to treat this disease can have many side effects. The aim of the present study was to construct a liposomal system containing essential oil of Trachyspermum ammi and investigate its physicochemical properties to combat trichomoniasis infection.

Liposomal vesicles were prepared using phosphatidylcholine (70%) and cholesterol (30%) by thin film method. In addition, the essential oil of Trachyspermum ammi was loaded into liposomes and the encapsulation efficiency of essential oil was calculated. Then, the size, surface charge and particle morphology of this system were investigated using Zeta Sizer and Atomic Force Microscopy and pattern of essential oil release was evaluated at 35 oC and pH = 6 for 24 h.

Liposomal vesicles containing essential oil had a size of 110.1 nm, 40.12±2.71% essential oil loading, and zeta potential of -9 mV. The liposomal system is controlled release and releases all essential oils within 24 hours. Atomic force microscopy also confirmed the formation of liposomes and appropriate particle morphology.

In the present study, the liposomal system containing the essential oil of Trachyspermum ammi had a relatively high loading percentage and surface charge verification confirmed that the nano-carrier is anionic. Thus, given the slow and complete release of the essential oil within 24 hours, this liposomal nano-carrier can be a suitable carrier for delivering the essential oil to Trichomoniasis parasite.

Elastin-like polypeptide (ELP) s an artificial biopolymer and a pentapeptide with repeating motif of Val-Pro-Gly-Xaa-Gly (VPGXG). The structure is derived from extracellular cellular matrix (ECM) elastin. Protein-based polymers, which are composed of repeat units of natural or unnatural amino acids, have recently emerged as a promising new class of materials. The unique properties of ELPs such as being genetically encoded in a heterologous host (e.g., bacteria or eukaryotic cell), excitability under the influence of environmental factors, biocompatibility and biodegradability made them popular in a wide variety of biomedical applications.
In this study, 330bp GRGDS-ELP monomer gene (expressing21 pentapeptide) was oligomerized into the cloning vector pUC to produce 990bp trimer gene (expressing63 fusion pentapeptide).After confirming gene structure by sequencing, the gene sequence was subcloned into pET-MOD expression vector and transduced into the E.coli.
Recombinant protein expression was induced by IPTG and the accuracy of the recombinant protein was confirmed by SDS-PAGE and Western blotting.
With mentioned method, ELP was obtained for further studies