جستجوی مقالات مرتبط با کلیدواژه « نفروپاتی » در نشریات گروه « پزشکی »

Polyomavirus BK is one of the life-threatening infections in kidney transplant recipients. The activation of this virus can eventually lead to the loss of the graft. There are very few studies on the expression level of BK virus microRNAs. Therefore, in this study, we investigated the function of BK virus microRNAs and their effect on host cell microRNAs’ expression level. BK virus encodes two microRNAs, namely miR-B1-3p and miR-B1-5p. Therefore, it is crucial to study these microRNAs as markers of viral infection and their regulation. So far, there is no approved drug or vaccine to treat BK virus infection. Consequently, understanding the relationship between BK virus and host cell microRNAs is integral to the control of infection in kidney transplant patients.

In this study, ten tissue samples from kidney transplant recipients with symptoms of BK virus nephropathy, ten urine samples from kidney transplant patients without active BK virus infection, and 20 healthy individuals were included. The expression level of the studied microRNAs was measured in all the samples using SYBR Green Real-time PCR.

The results showed that the expression level of the studied microRNAs, including miR-B1-3p, miR-B1-5p, miR-155, miR-520, miR-10b, miR-30a in the tissue samples of kidney transplant patients with BK virus nephropathy symptoms were significantly increased compared to kidney transplant patients without active BK virus infection.

The assessment of some microRNAs, such as miR-520, miR-30a, and miR-B1-3p/5p, may assist in the prediction and prevention of BKPyV activation in KTRs, particularly in urine samples.

Diabetes is one of the chronic diseases and the most common disease related to metabolism in humans. Diabetic nephropathy, one of the dangerous complications of diabetes, has a relatively high prevalence in diabetic patients and causes kidney failure. Several factors have an effect on this disorder. The aim of this study was to determine the cumulative probability of occurrence of nephropathy complication of type 2 diabetes using survival analysis.

This study was a historical cohort study of survival analysis, which was conducted in a descriptive-analytical manner in 2019. The required sample size was estimated at 410 patients. Data were calculated using Stata.ver14 software and descriptive tests and then by Kaplan Meyer method and Cox survival proportional hazards model.

The variables of regular visits and smoking showed a significant relationship with the chance of nephropathy complications (p<0.05). Therefore, the chance of nephropathy complications in people who did not have regular visits was 1.03 times that of people who had regular visits. The Log-Rank test showed that the median survival time (months) of the occurrence of nephropathy is related to the variables of fasting blood sugar, blood pressure, systolic, and blood lipids.

With early diagnosis of diabetes and control of the factors that accelerate the development of diabetic nephropathy and effective and timely treatments, it is possible to reduce the progress of the disease and it can be used in the field of prevention and treatment of diabetic patients and prevention of subsequent lesions and complications.

IgA Nephropathy is the most common form of glomerulonephritis and the major cause of end-stage renal disease. Gross or microscopic hematuria is a common symptom of hematuria and occurs in less than 5% to 10% of patients with IGM with rapidly progressive glomerulonephritis. The aim of this study was to investigate the diagnostic biomarkers of this disease after biopsy.

IgA (N) Nephropathy is the most common glomerulonephritis in the world and is currently recognized as an important end-stage renal disease (ESRD). Hypertension, proteinuria greater than 1 g / day, and severe lesions in primary renal biopsies such as crescent and interstitial fibrosis are the major predictors of progression to ESRD. Despite its prevalence and clinical significance, there is no consensus for treating patients with risk factors for worse prognosis. Our aim is to describe cases of crescentic IgA N and emphasize the efficacy of immunosuppressive therapy here.

This is a case-control study of the physiological and pathologic features of a patient with clinical, radiologic, and treatment modalities of a patient with immunoglobulin A nephropathy without a history of renal disease.

IgA Nephropathy, first identified nearly four decades ago as one of the types of kidney disease, is one of the most common causes of primary glomerulonephritis in developing countries and is now one of the major causes (End - stage renal disease (ESRD).

Ochratoxin A (OTA) is a nephrotoxic mycotoxin, which is classified by IARC in the 2B category of carcinogenic factors in human bodies due to immunotoxic, mutagenic and teratogenic effects. OTA is known as the Balkan endemic nephropathy agent which causes renal cancer. Feeding animals with OTA-contaminated grains will make their milk and meat toxic enough to threaten human health. Pregnant and breast-feeding women as well as children who consume a large amount of milk and meat on a daily basis are threatened the most. OTA represents one of the most challenging issues in clinical medicine and global food safety because its harmful effects, including growing incidence of cancer, Alzheimer's and Parkinson's, infertility and inhibition of gluconeogenesis, are under serious consideration by researchers. In light of the significance of OTA health threats and FAO and WHO recommendations for OTA monitoring in grains, this study aims to examine OTA presence in rice and bread.

Background and Multiple lines of evidence demonstrated that Crocus sativus petals (saffron) have beneficial effects on some diseases such as diabetes. This study was carried out to elucidate the effects of ethanolic extract of SP on renal function in diabetic rats.
In this experimental study, streptozotocin (STZ)-induced diabetic male Wistar rats (180-200 g) were orally treated with normal saline or 100 or 200 mg/kg of SP extract once a day for 28 days. Finally, fasting blood sugar (FBS), urine volume, 24 h urine total protein (UTP), blood nitrogen urea (BUN), and plasma creatinine (Cr) were assessed biochemically, and qualitative renal histomorphological alterations were evaluated pathologically. Data was analyzed using ANOVA and Kruskal-Wallis tests in SPSS, version 22.
The FBS, urine volume, UTP, BUN, and Cr levels significantly increased in diabetic rats compared to those of the normal control group (P SP may have beneficial effects against diabetic nephropathy through reducing extracellular matrix accumulation and its antioxidant properties.

Diabetes is a multi-factorial metabolic disorder caused by defects in insulin secretion/function or both. Diabetes has become a global problem because of increasing trend of elderly, socio-economic transformation and demographic changes. This study tried to evaluate the effectiveness of integration of diabetes control program in health networks and utilization of family practice in caring diabetics.
This retrospective cohort study was conducted on 462 type 2 diabetic patients with no complication detected in screening program in rural areas of Kordkoy district, Golestan province. The diabetic patients were divided into two groups using stratified random sampling. The incidence rates of eye and renal failure, diabetic foot ulcer, stroke and heart attack were compared between two groups.
In this study incidence rate of retinopathy, nephropathy, diabetic foot ulcer, stroke and heart attack in patients were 9.7, 5.9, 3.5, 3.3 and 6.1%, respectively. Diabetic foot ulcers and heart attacks significantly correlated with quality of the care received (p The results of the study indicate that family practice plan was not successful enough to achieve its goals in preventing and reducing the short-term and long-term complications of diabetes in Kordkuy city.

Cisplatin is a widely used anti-cancer drug, which its application is limited by nephrotoxicity. In this study, the effect of pretreatment with different l-arginine doses on Cisplatin-induced renal functional injury was investigated.
63 male rats were divided into 7 groups: In groups 3, 4, 5 and 6, 60 min before the Cisplatin injection (5mg/kg); L-Arginine with doses of 50,100,200 or 400mg/kg was injected, respectively. In group7, normal saline was injected before Cisplatin administration. In groups 1 and 2, normal saline was injected instead of Cisplatin. In group 2, 60min before normal saline injection, 400mg/kg L-Arginine was administered and in group1, instead of L-arginine, normal saline was injected too. Injections were intraperitoneal. 72h after Cisplatin injection, blood sampling and plasma separation were done. Urine sample was collected 24 hours before blood sampling by metabolic cage. The mean of plasma urea and creatinine levels and creatinine clearance (ml/day.kg) and fractional excretion of Na (FENa, %) were compared among different groups as renal functional parameters.
In comparison to group 7, L-arginine injection in a dose of 400mg/kg led to significant amelioration of all parameters. 200 mg/kg L-arginine administration led to significant decrease in plasma urea level and FENa. 100mg/kg L-arginine caused significant improvement in fractional excretion of sodium. L-arginine injection with 50mg/kg dose, significantly ameliorate all renal function tests instead of creatinine clearance.
Pretreatment with L-arginine administration with 400 or 50 mg/kg doses, respectively, had the highest effect on reducing Cisplatin-induced nephropathy. L-arginine injection with intermediate doses i.e. 200 or 100 mg/kg had less effect in reducing Cisplatin-induced nephropathy and it needs more investigations.

Background and Diabetes is a health problem worldwide with increasing prevalence in most countries. Nephropathy is a common microvascular complication of diabetes. Diabetes is directly associated with oxidative stress and mitochondrial damage that result in renal tissue damage. This study evaluated the efficiency of nanoceria in attenuation of oxidative damage in renal isolated mitochondria of STZ-induced diabetes mice. In an experimental study, the mice were divided into five groups (n=6 per group). Diabetes was induced by a single dose of streptozocin (65mg/kg body wt, IP) and the mice with glucose concentrations exceeding 200 mg/dl were considered diabetic and were treated with nanoceria and vit E for 4 weeks. Afterwards, the animals were anaesthetized and kidney tissues were excised at 4°C and mitochondrial fraction was separated by different centrifuge technique. Finally, oxidative stress markers including measuring glutathione (GSH) content, lipid peroxidation (LPO), reactive oxygen species (ROS) and MTT test were assayed in isolated kidney mitochondria. The level of GSH and mitochondrial function significantly decreased in diabetic mice compared to those in control group (P<0.05). Also, significant increase in LPO and ROS formation were observed (P<0.05) in the renal mitochondria of diabetic mice that decreased significantly after nanoceria administration (P<0.05). Our findings showed the protective effects of nanoceria against renal mitochondria damage. Therefore, it could be used as a complementary therapy alongside other blood glucose-lowering drugs to reduce the rate of complications resulting from diabetes.

Diabetes mellitus is a metabolic disorder which its subsequent complications such as retinopathy, nephropathy, ulcers, disability, and amputation increase the burden of the disease. Patient-knowledge-improving programs are employed to prevent disease progression and to improve the patients’ quality of life. In this way, we need to characterize the groups of patients with urgent need for more and rich in content programs. In the present study, we used Piecewise regression to evaluate the trends in diabetic nephropathy prevalence among patients registered in Sedigheh-Tahereh Research Center and also identify patients needing more attention. This retrospective study was conducted on 1935 registered patients in the center during 1992-2010.Piecewise regression was fitted using Joinpoint program 3.5.3 to identify change points. The results showed an upward trend with 2 change points in females (p-value= 0.06). There was 1 change point with an increased trend in recent years in elderly patients, older than 70 years (p-value=0.038).The prevalence of diabetic nephropathy was in rise without any change point among patients with academic education. In addition, patients with family history of diabetes showed the decreased trend with 2 change points (p-value= 0.040). Hypertensive patients experienced a fall in the trend up to 1997 and then this reminded stable. Evaluating of a disease trend and its complications can lead to developing new theory and innovation. However, this is suggested to conduct further studies to exploring other subgroups and discovering the reasons for increased trend of disease in some subgroups.

Administration of contrast media (CM) results to an iatrogenic complication named contrast-induced nephropathy (CIN). CIN, defined as an increase in serum creatinine >25% of the baseline or (>0.5 mg/dL) within 2-3 days of intravascular contrast media, using in the absence of another renal disease. Incidence of CIN in the general population is reported overall to be 0.6-2.3%. Incidence of CIN is 10-20% in adults who underwent cardiac angiography and catheterization. Results from other studies in children are limited. The purpose of this study was to determine the frequency of CIN in children with congenital heart disease. Subjects and One hundred children (ages ranging from 1 months to 20 years) during a period of 15 months (July 2011 and September 2012) were enrolled. Blood urea nitrogen (BUN), serum creatinine (Cr), sodium (Na+) and potassium (K+) were measured before angiography. These measurements were repeated 24 and 72 hours after angiography. Twenty four patients had >25% rising of creatinine from their baseline level. Base line level of BUN in patients with CIN were 12.04 mg/dl before the procedure that increased to 13.6 mg/dl at 24 hour and 13.88 mg/dl at 72 hours after procedure (P = 0.022). Results of this study showed that the frequency of CIN is relatively high in children with congenital heart disease who underwent cardiac catheterization and is comparable to other reports in adults.

Polyomaviruses (BK) is a comprehensive infection with more than of 80% prevalence in the world. One of the most important reasons of BK virus nephropathy is in the renal transplant recipients and rejection of transplanted tissue between them. Non Coding region of this virus play a regulatory role in replication and amplification of the virus. The aim of this study was to evaluate the genetic patterns of this area in renal graft at Namazi Transplantation Center، Shiraz، Iran. In the present experimental study، 380 renal allograft serums were collected. DNAs of 129 eligible samples were extracted and evaluated using a virus genome. The presence of the virus was determined by qualitative and sequencing. Of these، 129 samples were tested for the presence of virus according to the condition study، using quantitative، qualitative genomic amplification and sequencing. The study showed symptoms of nephropathy، 76 (58. 9%) of them were males and 46 (35. 7%) were females with the mean age 38. 0±. 089 years of age. In general، 46 patients (35. 7%) percent) were positive for BK Polyomaviruses. After comparing the genomic sequence with applications of molecular they were categorized in three groups and then recorded in gene bank. About 35% of renal transplant recipients with high creatinine levels were positive for the presence of BK virus. Non-coding region of respondents in the sample survey revealed that among patients with the most common genotypes were rearranged the entire transplant patients were observed at this tranplant center. Examination of these sequences indicated that this rearrangments had a specific pattern، different from the standard strain of archaea type.

Introduction & Diabetes is one of the most common glandular diseases in the world. Patient with diabetes are more than other individuals with eye problems, nervous, cardiovascular, renal insufficiency. According to the importance of the subject and high prevalence of diabetes and its complications, the current study was designed and done with the aim of determinants of Prevalence of chronic complications of type 2 diabetes and its related factors in referred diabetes patients to Freydonkenar diabetes center. This is a descriptive-analytical study that was done in referred diabetes patients with type 2 diabetes to Freydonkenar diabetes center and 400 records of patient were selected via randomly method. The research instrument was a self-made check-list including demographic characteristics and diabetes complications. Collected data were analyzed by using SPSS18 and Chi-squared & t test. from 400 Records of patients, 77 patients were (19/3%) males and 323 (80/8%) female 90.5% of the patient suffers from chronic complications of diabetes. 65.8% suffer from neuropathy, 26.8% eye complications, 14.5% renal complications and 38.8 cardiovascular complications and 50.5% other complications. The results showed Prevalence of diabetes complications is high. so we recommended appropriate intervention such as educational intervention for promoting knowledge of the patients and screening for preventing complications of the disease.

Diabetes is the most prevalent metabolic disorder. This disorder can affect on different organs of body، and as a result، it can affect the quantity and quality of life. Not having enough knowledge on their own health، one is not able to retain and promote his/her own health. The aim of this study is to determine health literacy among diabetes type 2 patients in Imam Khomeini Hospital، Saqez، Iran.

The study is a cross-sectional one in which diabetes type 2 patients were participated. Data collection were done by using TOFHLA، and demographic questionnaires. Data analyses was done by using independent t-test and analysis of variance.

Totally، 1563 diabetes type 2 patients participated in the study. Mean score of health literacy was 27. 19. A significant relation exist between health literacy with age، sex and education levels. (P<0. 05). The results of the study didn''t reveal any significant relation between health literacy and nephropathy and retinopathy.

It is concluded that the level of health literacy is very low، therefore it is crucial to promote health literacy of diabetes type 2 patients.

This study was done to investigate the effect of lovastatin therapy on paraoxonase 1 (PON1) and arylesterase (ARE) activities، and low-density lipoprotein cholesterol (LDL-C) susceptibility to oxidation in people with type 2 diabetic nephropathy (T2DN). Lovastatin (20 mg/day) was administered to 30 people with T2DN for 90 days and then withdrawn for 30 days. PON1 and ARE activities were measured by the spectrophotometric method. Susceptibility of LDL-C to oxidation was determined as the production of conjugated dienes. After 90 days of lovastatin intervention، PON1 and ARE activities and LDL-C lag phase were significantly increased (p=0. 004، 0. 002، and b0. 001). After 30 days of lovastatin withdrawal، PON1 and ARE activities and LDL-C lag phase had not changed significantly. Lovastatin therapy improves PON1 and ARE activities، and LDL-C susceptibility to oxidation. Despite withdrawal of lovastatin، PON1 and ARE activities، and LDL-C susceptibility to oxidation remain unchanged for a long period of time in people with T2DN.

Introduction & The study of biopsychosocial factors influencing nephropathy as a most serious complication of type II diabetes is important. This study aimed to investigate risk factors accompanied with nephropathy in patients with type II diabetes based on the biopsychosocial model. In a cross-sectional descriptive study, 295 patients with type II diabetes were selected by convenience sampling in Tehran Shariati hospital outpatient clinics. The data were collected by demographical information questionnaire along with disease characteristics and depression anxiety stress scales (dass), quality of life scale (who- qol- bref), diabetes self-management scale (dsms), and diabetes knowledge scale (dks), then analyzed by chi-square, independent t-test and logistic regression with pasw software. Hypertension (OR=3.841 & P<0.003), glycated hemoglobin (OR =7.898 & P<0.001), body mass index (OR=5.488 & P<0.001), diabetes self-management (OR=3.102 & P<0.001), depression (OR=2.574 & P<0.007), quality of life (OR=2.836 & P<0.001), and diabetes knowledge (OR=2.941 & P<0.001) can significantly help differentiate diabetic patients with nephropathy from diabetic patients without nephropathy (P<0.001). Sex, age, diabetes duration and stress do not play any significant role in the prediction of nephropathy incidence in this model (P>0.05). It is important to pay attention to hypertension, glycated hemoglobin, body mass index, diabetes self-management, depression, quality of life, and diabetes knowledge for therapeutic intervention programming and diabetes complications control protocols for diabetic patients.

There are few reports regarding the effect of long-term soy protein consumption among type 2 diabetic patients with nephropathy. This study was conducted to elucidate the effects of soy consumption for a long period of time on cardiovascular risks, C-reactive protein (CRP) and kidney function indexes among type 2 diabetic patients with nephropathy. This long-term randomized clinical trial was conducted among 41 type 2 diabetic patients with nephropathy (18 men and 23 women). Twenty patients in the soy protein group consumed a diet containing 0.8 g protein/kg body weight (35% animal proteins, 35% textured soy protein, and 30% vegetable proteins) and 21 patients in the control group consumed a similar diet containing 70% animal proteins and 30% vegetable proteins for 4 years. Soy protein consumption significantly improved cardiovascular risks such as fasting plasma glucose (mean change in the soy protein versus control groups: −18 ± 3 vs. 11 ± 2 mg/dl; P = 0.03), total cholesterol (−23 ± 5 vs. 10 ± 3 mg/dl; P = 0.01), LDL cholesterol (−20 ± 5 vs. 6 ± 2 mg/dl; P = 0.01), triglyceride (−24 ± 6 vs. −5 ± 2 mg/dl; P = 0.01) and serum CRP (1.31 ± 0.6 vs. 0.33 ± 0.1 mg/l; P = 0.02) concentrations. Significant reductions were also seen in proteinuria (−0.15 ± 0.03 vs. 0.02 ± 0.01 g/day; P = 0.001) and urinary creatinine (−1.5 ± 0.9 vs. 0.6 ± 0.3 mg/dl, P = 0.01) by consumption of soy protein. Long-term soy protein consumption has beneficial effects on cardiovascular risk factors and kidney-related biomarkers among type 2 diabetic patients with nephropathy.

Diabetic nephropathy (DN) is a common cause for end-stage renal disease in developing countries. The characteristic features of this disease are persistent albuminuria and reduction of serum albumin. We aimed to demonstrate the preventive effect of garlic juice on damages in DN in rats with streptozotocin (STZ)-induced diabetes. Forty male rats (250±20) were divided into 5 groups as follows: 1- Group normal (N) 2- Group Normal+Garlic (N+G) 3- Diabetic (D) 4- Group diabetic+garlic before (D+Gb) (receiving garlic before STZ injection) 5- Group diabetic+garlic after (D+Ga) (receiving garlic after STZ injection). Garlic juice was given by gavage (1ml/100gBW). Diabetic rats showed an increase in serum glucose, urinary volume, and urinary albumin to creatinine ratio (Alb: Cr), urinary albumin excretion, and decrease in serum albumin. Garlic juice significantly attenuated the diabetes-induced increase in albumin clearance and urinary albumin excretion in the diabetic rats. The diabetic group receiving garlic before STZ injection showed more amelioration in complications than that receiving it after STZ injection. In this study for the first time we showed that administration of garlic juice could play a preventive role on diabetic nephropathy in rats.

Today, oxidative stress and free radicals are known as important factors of pathogenesis caused by diabetes. In the present study the effect of aqueous extract of Cynodon dactylon rhizome on lipid peroxidations in plasma and nephropathy induced by diabetes has been studied. In this experimental study, 30 adult male Wistar rats where randomly divided into 5 six member groups. The control group rats were injected with physiological serum. Group II rats, contracted diabetes through being injected 70 mg/kg streptozotocin (STZ) intraperitoneally. For 4 weeks, the rats of the third, fourth and fifth groups, were fed aqueous extract of Cynodon dactylon rhizome, 50, 250 and 500 mg/kg respectively. After the end of treatment, blood from the heart of rats was obtained and after opening the abdominal cavity their kidneys were removed and put in 10% buffered formalin solution. Malondialdehyde (MDA) of plasma was measured as an indicator for lipid peroxidations. After preparing kidney sections and painting them, they were studied in terms of changes in glomerular and tubular structures. Plasma MDA levels in diabetic rats, treated with 500 mg/kg extract, decreased significantly compared to untreated diabetic group (P<0.01). In untreated diabetic rats basement membrane thickening, dilatation of the urinary space, and the hyalinized artery walls, were observed. This study showed that aqueous extract of Cynodon dactylon rhizome reduces lipid peroxidation and decreases diabetic nephropathy.

Reactive oxygen species (ROS) and oxidative stress play important roles in the development of diabetic complications. Regarding the importance of medicinal herbs, the effect of Sumac extract on lipid peroxidation and diabetic nephropathy was explored in this study. In this experimental study, 30 adult male Wistar rats were divided into 5 six member groups. Control rats were injected with the necessary volume of physiological serum. Group II contracted diabetes by being intraperitoneally injected 120 mg/kg Alloxan.The rats of the third, fourth, and fifth group, in addition to the same treatment, were fed 50, 100 and 250 mg/kg of aqueous extract of Rhus-coriaria L. respectively for 4 weeks. After 28 days of treatment, blood samples were collected from the heart. Plasma malondialdehyde (MDA) concentration, as an end product of lipid peroxidation, was determined. In addition, kidney tissues were removed and prepared for histological analysis. The obtained data was analyzed by means of one-way analysis of variance (ANOVA) and Tukey statistical test Treatment with the extract of Sumac caused a significant reduction in MDA level in diabetic groups treated, compared with the control and diabetic groups (P<0.05). Moreover, treatment with the extract led also to a significant improvement of side-effects of diabetes mellitus on the distal arterioles of renal tissues in diabetic rats. It was found that sumac decreases lipid peroxidation and kidney damage in diabetic rats.

Fabry disease is a X-linked lysosomal storage disorder due to alpha galactosidase A deficiency leading to abnormal accumulation of glycosphingolipids in different parts of body. This case report introduces a 35-year-old man with diffuse keratotic erythematous papules. Histopathological evaluation of the skin biopsy suggested the diagnosis of angiokeratoma. With attention to his nephropathy and the same skin lesions and renal involvement in his brother, which led him to receive renal transplantation, the diagnosis of Fabry disease was suggested.