جستجوی مقالات مرتبط با کلیدواژه "ولوواژینیت کاندیدیایی" در نشریات گروه "پزشکی"

Vulvovaginal candidiasis, as a prevalent vaginitis, is developed by Candida albicans. Regarding to the high incidence of this fungal infection and the antifungal activities of Cuminum cyminum, the preparation of vaginal suppositories containing its essential oil, has been considered as the main goal of the present investigation.

The constituents of C. cyminum essential oil were identified by gas chromatography-mass spectrometry. The minimum inhibitory concentration (MIC) of the essential oil and the related formulation were determined by the cup plate method.

Vaginal suppositories containing 0.1 ml of C. cyminum essential oil per suppository base (2 gr) (Polyethylene glycol) were prepared and their appearance, mass uniformity, hardness, disintegration time, content and uniformity were evaluated. The stability of suppositories containing C. cyminum essential oil stored at 4, 25 and 40°C was investigated after 1, 2, and 6 months. It was found that storage at lower temperatures (4 and 25°C) did not cause any damages to the formulation.

Due to the high quality of the formulated suppositories, it would be suggested as a successful replacement in the treatment of vaginitis caused by Candida albicans, after approving its efficacy in future clinical studies.

Vulvovaginal Candidiasis (VVC) affect millions of women annually. Candida albicans is the most common cause of VVC. Secreted aspartyl proteinases (SAPs) are among the most important virulence factors in Candida species. The SAP3 enzyme is effective in the initial development of VVC infection due to its role in the adhesion of Candida albicans. The aim of this study was to determine the presence of SAP3 gene in Candida albicans strains isolated from patients with Vulvovaginal Candidiasis. In this cross-sectional study, vaginal secretion samples were collected from 268 vaginitis patients referred to Amiralmomenin Hospital of Gerash city, Fars province, Iran from March to August 2018. After direct microscopic examination, all samples were cultured on sabouraud dextrose agar medium with chloramphenicol (50mg/L). Candida strains were identified using standard phenotypic and sugar assimilation tests (API20C). Genomic DNA extraction and the presence of SAP3 gene were performed using chloroform-phenol-isoamyl alcohol and polymerase chain reaction (PCR) methods, respectively. Out of 268 samples, 79 (29.47%) cases were positive for Candida species under direct microscopy and culture results. 48 (60.75%) and 31(39.24%) Candida albicans strains and Non-albicans candida species were isolated, respectively. The results of PCR showed the presence of SAP3 gene in 47 (97.91%) Candida albicans strains. It can be concluded that Candida albicans were more likely to cause Vulvovaginal than other Candida species. The explanation for this is the presence of the SAP3 gene in most Candida albicans strains.

Candidiasis is a fungal infection which is caused by Candida spp. Candida albicans is the most common agent of candidiasis. This infection may cause various side effects during pregnancy including prematurity, chorioamnionitis, candidal pneumonia, and systemic candidiasis of infants. This research was conducted for determining the prevalence and antibiotic resistance pattern of Candida spp, collected from pregnant women admitted to health centers in Ardabil, Iran.
Totally, 408 subjects were included in this study. Demographic data and risk factors were recorded using a questionnaire. Two swab samples were taken from vulvovaginal mucus. One swab was used for preparing smear and direct microscopic examination and the second one used for cultivating the specimen. After identification of Candida spp., antimicrobial resistance pattern was determined by disk diffusion method against Fluconazole, Ketoconazole, Clotrimazole, Nystatin and Amphotericin B. Results were interpreted according to CLSI guidelines. The data were analyzed by χ2 and t-test using SPSS-19.
Out of 408 subjects, 143 cases (35%) were positive for candida spp. The Candida albicans with 119 (83.2%) cases was the most prevalent species followed by Candida glabrata, Candida krusei, Candida parpsilosis and Candida tropicalis.According to disk diffusion test, overall 116 (81.1%) isolates were resistant to Fluconazole, 100 (69.9%) to Ketoconazole, 67 (46.9%) to Clotrimazole and 25 (17.5%) to Amphotericin B. Candida spp. had a highest sensitivity (118, 82.5%) to Nystatin. For Candida albicans 97(81.5%) isolates were resistant to Fluconazole and 99(83.2%) isolates to Nystatin. For Candida glabrata 10 (90.9%) isolates were resistant to Fluconazole, and 9(81.8%) sensitive to Nystatin.
According to the results of this study, vulvovaginal candidiasis is prevalent among pregnant women in Ardabil and isolates were significantly resistant against commonly used antifungal drugs. Nystatin was the most effective against Candida spp. As antibiogram for fungal agents is not routinely performed, the similar periodical studies could be useful for choosing appropriate antibiotics in treatment of vulvovaginal candidiasis.

Vulvovaginal candidiasis occurs due to the overgrowth of candida in genital system mucosa of females. Symptoms and signs of vulvovaginal candidiasis are unspecific, therefore its diagnosis based on them (symptoms and signs) is not certain. The aim of this research was comparison the result of indirect immunofluorescence and ELISA with culture and direct microscopy examination in patient with vulvovaginal candidiasis. This was acomparative-descriptive study that was performed on 87 patients and 50 normal cases. All specimens were examined under direct microscopy, culturing and complimentary test to differentiate the candida species from each other. Serological tests such as indirect immunofluorescence and ELISA were performed on sera of the patients. To compare the quantitative and qualitative data in order t-student test, chi-square and exact fisher test were used, if necessary. Out of 87 specimens, 50 cases were diagnosed as vulvovaginal candidiasis. In order, frequency of the isolated pathogens were: C.albicans, C.glabrata,C.kefyr, C.inconspicua and C.famata. Also in order frequency of control groups were: C.albicans, C.glabrata and C.kefyr. In this study all of normal cases were negative in indirect immunofluorescence test and in patients group 42 person(84%) were positive and 8 (16%) negative. Control groups were negative in ELISA and in patient group 40 person(80%) were positive and 10 person(20%) negative. It seems in cases that are not possible to perform direct microscopic and culturing methods, ELISA and indirect immunofluorescence can be used as a replacement method.

Background and Vulvovaginal candidiasis is one of the most common infections in women and is caused by various types of candidiasis with different patterns of resistance against drugs. The present study was conducted to determine the various species of candidiasis in women admitted to Mobini Hospital in Sabzevar, Iran in order to make preventive recommendations for medical doctors. Methods and Materials: This cross-sectional descriptive analytical research was conducted on 231 women admitted to Mobini Hospital in Sabzevar, Iran, complaining of vaginal secretions. After getting the ethics committee permissions and obtaining the informed consent of the participants, at the same time as measuring vaginal pH, two swaps were used for collecting samples of secretions from lateral and posterior fornix. They were examined for yeasts and were then cultured in saburu dextrose. Grown yeasts were detected based on their macroscopic and microscopic features, tubulation in blood serum and biochemical characteristics using Yeast Plus System. The obtained data were analyzed in SPSS 11 using chi-square and bi-dimensional tables. Positives results in direct examination results were 7.35%, and in culture the positive results were 26.8%. In detecting processes, candida albicans yeast (38.7%), Candida kefyr (17.7%), Saccharomyces cerevisiae (14,5%); candia tropicalis (9.6%), Candida glabrata (8%), candida cruse (3.2%) were isolated with other non-pathogenic yeasts including Candida rugosa, Candida lipolytica, trycospron bejli, Blastoschizomyces capitatus (6.4%). Non-pathogenic yeasts were isolated from patients with defective immunity systems, and need to be considered in patients with vaginal infections.