جستجوی مقالات مرتبط با کلیدواژه « کولینرژیک » در نشریات گروه « پزشکی »

Glycyrrhiza glabra (Licorice) has been traditionally used as a medicinal plant in Iran for treatment of diseases such as gastric ulcer and relieve intestinal spasms.
In the present study, the effect of hydro-alcoholic extract of licorice rhizome on mechanical activity of isolated colon of male rats has been studied.
In this experimental study, the colon tissue of 10 adult male rats were dissected and separated into two groups: experimental and control. Each group including ten strips of tissue. The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution (37 °C, pH=7.4). Their mechanical activity were recorded by power lab AD instrument in basal condition, and after administration of acetylcholine (4×10-6M) and Atropine (10-6M) in the presence and absence of licorice rhizome extract (0.036 mg/ml). Also, the mechanical activity of control group segments were recorded at the same condition with extract solvent (ethanol %70).
A significant decrease in mechanical activity was found after administration of hydro-alcoholic extract of licorice compared with the control group. Also, a significant decrease of mechanical activity was seen in the presence of extract and acetylcholine compared to the control group. While the mechanical activity of tissue didn’t significant changed in the presence of Atropine and extract between experimental and control groups.
We can conclude that hydro-alcoholic extract of licorice has modifying effect on colon motility, and this activity maybe occurred independent of the cholinergic system.

The licorice (Glycyrrhizaglabra) rhizome has been widely used in traditional medicine for treatment of gastrointestinal diseases such as gastric ulcer and relieve intestinal spasms. In the present study, the effect of hydro-alcoholic extract of licoricerhizome on mechanical activity of isolated colon of male rats has been studied.
Adult male rats were anesthetized by ethyl ether, their abdomen opened, and colon tissues were removed and divided into 1 cm segments. The segments were connected to a force transducer longitudinally and inserted to an organ bathe contained oxygenated Tyrode solution (37 °C, pH=7.4). Their mechanical activity of ileum was recorded by power lab AD instrument in basal condition, and after administration of L-NAME (10-4M), acetylcholine (4×10-5M) and Atropine (10-5M) drugs in the presence and absence of licorice rhizome extract were recorded (0.036mg/ml). Also, the mechanical activity of control group segments were recorded at the same condition with extract solvent (ethanol %70).
A significant decrease in mechanical activity of the isolated colon occurred after administration of hydro-alcoholic extract of licorice rhizome compared to the control group (p≤0.05). Also, a significant decrease was seen in mechanical activity occurredin the co-administration of extract and acetylcholine compared to the control group. The mechanical activity of tissue was not significantly changed in the presence of Atropine and extract between experimental and control groups. The mechanical activity of ileum tissue was not significantly changed in the co-administration of L-NAME and extract between experimental and control groups.
We can conclude that hydro-alcoholic extract of licorice has modifying effect on colon motility, and this activity may be occurred independently in the nitrergic and cholinergic systems.

Recent studies showed that nitrergic system have specific modulatory effects on electrophysiological properties of atrioventricular (AV) node. The aim of this study was to determine the effects of nitric oxide (NO) on the electrophysiological properties of isolated rabbit AV node and to investigate the role of adrenergic and cholinergic receptors in the mechanism of its action. In our laboratory, an experimental model of isolated double-perfused AV-node of rabbits weighing 1.5-2 kg was used. Specific experimental protocols of recovery, Facilitation, Fatigue and Wenckbach were applied in both control and in the presence of the drug. A total number of 35 rabbits were divided randomly into the following groups (n=7): 1) L-Arg (NO donor) (250, 750 and 1000 μmol), 2) L- NAME, a NO synthesis inhibitor (25, 50 and 100 μmol), 3) L-Arg + L- NAME, 4) Nadolol (1 μmol), 5) Atropine (3 μmol). All data were shown as mean ± SE. The level of statistical significance was set at p<0.05. Our results revealed the depressant effect of L-Arg on the basic and rate-dependent electrophysiological properties of AV-node. L- NAME did not deteriorate the effects of L-Arg on the basic and rate-dependent properties, nevertheless, at high concentration (100 μmol) it had a direct inhibitory effect on the AV-node. Nadolol and atropine could prevent the effects of NO on the basic nodal characteristics and the fatigue phenomenon, respectively. Nitergic system can affect basic and rate-dependent electrophysiological properties of the AV-node through adrenergic and cholinergic receptors.