جستجوی مقالات مرتبط با کلیدواژه « کیست فولیکولار » در نشریات گروه « پزشکی »

Background and Keratocystic odontogenic tumor tends to recur and behave more aggressively than follicular cysts and dental follicles. Eosinophils are multifunctional leukocytes, but their role in oral lesions is controversial. Current study aimed at assessing the density of eosinophils in aggressive odontogenic lesions.
In this descriptive-analytical cross-sectional study, paraffin blocks were obtained from Babol Dental School archives including 16 cases of keratocyst,19 follicular cyst, and 12 dental follicle and their demographic characteristics were extracted. The overall density of eosinophils and density of eosinophils in adjacent areas and away from odontogenic epithelium were examined in slides stained with Congo red in 10 microscopic fields (40x).
Eosinophil density was 0.08±0.3 in dental follicles, 1.7±2.1 in follicular cysts, and 6.14±5.6 in keratocystic odontogenic tumors. The eosinophil density in areas adjacent to odontogenic epithelium in keratocyst and follicular cysts was greater than that in areas away from odontogenic epithelium (P>0.001).
Based on current study, eosinophils appear to influence the pathogenesis of follicular cysts and keratocystic odontogenic tumors. Their increased density in keratocysts, especially in the vicinity of odontogenic epithelium, is effective in its aggressive behavior.

Ameloblastoma is a benign epithelial odontogenic tumor that exhibits a more aggressive behavior than odontogenic keratocyst and follicular cyst. The purpose of this study was to determine and evaluate histomorphometric characteristics and blood vessels pattern adjacent to and far from odontogenic epithelium on the clinical behavior of the above odontogenic lesions with immunohistochemistry and morphometry method. In this descriptive-cross-sectional study، 45 paraffin blocks of mentioned lesions were selected and marked immunohistochemically with CD34. From three HPFs (High Power Fields) in each slide which had more density of blood vessels in adjacent to and far from odontogenic epithelium، photography was done by a camera joined to a microscope with 40 times magnification. Through the software Motic plus 2، the internal and external diameters، section surface and thickness were determined. Also the blood vessel pattern was determined in the mentioned lesions and was compared through ANOVA، t-test and Multiple Comparison Test. The external and internal diameters and sectional surface in the regions far from the odontogenic ephithelium in ameloblastoma was greater than keratocyst (P<0. 001) and dentigerous cysts (P=0. 001) but there was no significant statistical differences seen in the thickness of blood vessels in the regions far from the odontogenic ephithelium in three lesions (P=0. 05). Significant statistical difference was not seen in external (P=0. 8) and internal (P=0. 8) diameters، sectional (P=0. 6) surface and blood vessels thickness in the adjacent regions of odontogeni ephithelium in these three lesions. The pattern of blood vessels in ameloblastoma was circumferential and in the keratocyst and follicular cyst، it was directional. It seems that morphometric characteristics of blood vessels (internal and external diameters، sectional surface) in areas far from odontogenic ephithelium and the type of blood vessels pattern affects clinical aggressive behavior of ameloblastoma more than keratocyst and follicular cyst.

Ameloblastoma is a benign epithelial odontogenic tumor that exhibits a more aggressive behavior than follicular cyst and keratocystic odontogenic tumor. The purpose of the current study was detection and evaluation of microvessel density (MVD) effect on growth and clinical behavior of above odontogenic lesions with immunohistochemistry. In this descriptive-analytic cross-sectional study, 45 paraffin blocks of mentioned lesions were selected and stained immunohistochemically with CD34. The mean MVD of each lesion in three microscopic fields was calculated separately and was evaluated in the two areas, adjacent and far from odontogenic epithelium and then were compared with one another. Mann-Whiteny and Kruskal Wallis tests were used for the statistical analysis. Mean microvessl density was 40.8±15.9, 25.4±5.3, and 9.4±3.52 in ameloblastoma, keratocystic odontogenic tumor, and follicular cyst, respectively.Mean microvessl density difference between the above mentioned lesions was statistically significant (P<0.001). In all the odontogenic lesions, the microvessel density adjacent to odontogenic epithelium was higher than the areas far from the epithelium (P=0.001). The increase in microvessel density in ameloblastoma compared to keratocystic odontogenic tumor and follicular cyst can be one of the main factors in ameloblastoma aggressive behavior. Angiogenesis adjacent to odontogenic epithelium has a more prominent role in growth of follicular cyst and progression and aggressive behavior of odontogenic tumors.

Odontogenic keratocyst is a developmental odontogenic cyst with aggressive behavior and tendency to high recurrence. The cyst’s epithelium shows more potential for growth than follicular cyst. On the other hand, BCL-2 is an anti apoptotic protein that can increase the longevity of epithelial cells. The purpose of this investigation was to compare the immunoreactivity in different layers and linings of both epitheliums of the follicular cyst and odontogenic keratacyst using BCL-2 anti-apoptotic marker. A retrospective descriptive study design was employed in order to evaluate 20 paraffin blacks of odontogenic keratocyst and 20 paraffin blocks of follicular cyst. The immunohistochemistry staining method was used together with BCL-2 marker(Anti BCL-2, Clone 124, Isotype: IgG, Kappa, Dako, Denmark).The cytoplasms of epithelial cells were immunostained in different layers and all linings of the epithelium for both cysts, in 1000 epithelial cell counts. The collected data was analyzed using SPSS statistical software, and Roc Curve analysis as well as student T-test in order to prepare and report the results. Immunoreactivity with BCL-2 marker was 98.5 (±1.4%) in Basal layer, 12.1 (±3.2%) in intermediate, and null (0) in the surface layer of odontogenic keratocyst. Immunoreactivity with BCL-2 marker in basal layer was 2.1 (±1.9%) but the surface and intermediate layers did not immunostained with BCL-2 marker. The mean number of epithelial cells, positive for BCL-2, in all linings of follicular cyst was 0.7 (±0.63%) and in odontogenic keratocyst was 36.8 (± 1.5%). The mean immunoreactivity difference in layers of odontogenic keratocyst was statistically significant (P<0.001). However, the mean difference in immunoreactivity with BCL-2 marker was not significant (P>0.05) in different layers of follicular cysts. Based on the results of this study, BCL-2 seams to be involved in pathogenesis of the odontogenic keratocyst. Likewise, the over-expression of BCL-2 marker in basal layer of odontogenic keratocyst was associated with increased recurrence and aggressive behavior of odontogenic keratocyst when compared with follicular cyst. Therefor, it can be suggested that the overexpression of BCL-2 in basal layer of odontogenic keratocyst can be considered as a useful marker to differentiate it from follicular cyst.