جستجوی مقالات مرتبط با کلیدواژه « 2dg electrophoresis » در نشریات گروه « پزشکی »

Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding. The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks. The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values. This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity.

Glioblastoma multiforme (GBM) or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. Several of the molecular alterations detected in gliomas may have diagnostic and/or prognostic implications, proteomics has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses. We extracted proteins of tumor and normal brain tissues and then evaluated the protein purity by Bradford test and spectrophotometery method. In this study, we separated proteins by two-dimensional (2DG) gel electrophoresis method and the spots were then analyzed and compared using statistical data and specific software. Proteins clustering analyses were performed on the list of proteins deemed significantly altered in glioblastoma tumors (t-test and one-way ANOVA; P < 0.05). The 2D gel showed totally 876 spots. We reported, 172 spots exhibited different in expression level (fold > 2) for glioblastoma. On each analytical 2D gel, an average of 876 spots was observed. In this study, 188 spots exhibited up regulation of expression level, whereas the remaining 232 spots decreased in glioblastoma tumor relative to normal tissue. Results demonstrate that functional clustering (up and down regulated) and principal component analysis has considerable merits in aiding the interpretation of proteomic data. At the core of proteomics was 2D gel electrophoresis witch permitted the separation of thousands of protein, High resolution 2DE can resolve up to 5,000 proteins simultaneously. Using cluster analysis, we can also from groups of related variables, similar to what you do in factor analysis.