جستجوی مقالات مرتبط با کلیدواژه « 4t1 cell line » در نشریات گروه « پزشکی »

 Alpelisib and Ursolic acid are two compounds that have been shown to have potential as anti-cancer agents. Alpelisib is a selective inhibitor of the PI3K pathway, while Ursolic acid is a natural pentacyclic triterpene compound found in various plants that reveals anti-cancer, antioxidant and anti-inflammatory characteristics. The hypoxia-inducible factor 1α (HIF1α) is a transcription factor that plays a key role in regulating tumor cell survival, apoptosis, tumorigenesis and growth in low-oxygen environments. This study aims to determine the effects of Ursolic acid and Alpelisib on the expression of HIF1α gene on 4T1 cell line.

 In the current experimental study, IC50 concentrations of both Ursolic acid and Alpelisib were determined on 4T1 cells. Then cells were treated with determined IC50 concentrations of Ursolic acid, Alpelisib and the combination of half of the IC50 concentration of both drugs for 24 hours. After the treatment, viability was assessed with MTT assay and the expression of HIF1α gene was appraised by Real-time PCR. Finally, statistical analysis was accomplished by ANOVA using GraphPad Prism 8.4 software.

 The results of this study showed that the anti-proliferative effect of the drug combination was synergistic and concentration-dependent. The maximum decrease (74.17 % with UA and 64.04 % with Alp) in viability was observed in high doses of treatment with drugs. IC50 values of Ursolic acid and Alpelisib were 168.314 µM and 6.377 µM, respectively. Based on the real-time PCR results, HIF1α gene expression was significantly decreased in both single- treatment and combination groups, compared to the control group (P<0.05).

 The results of this study showed that both Alpelisib and Ursolic acid alone or in combination with each other could have anti-cancer effects by reducing HIF1α gene expression. Further studies are needed to determine the efficacy and safety of these compounds in vivo.

 Induction of Th1 responses against tumor antigens may be a useful strategy to control malignancy. In this respect, previous studies have shown that beta-adrenoreceptor antagonists can promote cellular immune responses.

 This survey was done to evaluate the beneficiary of a new immunotherapy method against breast cancer made by mixing heated 4T1 cells and propranolol, as an adjuvant.

 Subcutaneously injected live 4T1 cells (1 × 104) were used to induce breast cancer in six to eight-week-old female Balb/c mice. when all mice had a palpable tumor, immunotherapy was dawned. Mice in the treatment groups were vaccinated, twice at a one-week interval, with the extract of heated 4T1(1 × 105) either alone or in combination with propranolol (6 mg/kg). Negative control mice received phosphate-buffered saline (PBS) as the same schedule. One-half of the mice were euthanized one week after the last vaccination to investigate the immune response profile. Other animals were kept until death occurred spontaneously.

 Combined immunotherapy with propranolol and extract of heated 4T1 had synergistic effects, causing a more desirable survival curve and slower tumor growth when compared to other tumor-bearing mice receiving only heated 4T1 or PBS. Furthermore, combined immunotherapy significantly augmented the production of IFN-γ nitric oxide production, respiratory burst, and cytotoxicity of natural killer cells in the splenocyte culture of tumor-bearing mice. Conversely, combined immunotherapy significantly regressed the production of TGF-β and IL-10 in the splenocyte population compared to cytokine production by splenocytes from other groups.

 Combined heated 4T1 cells with propranolol promote beneficial outcomes in the animal model of breast cancer.

Cancer is amongst the leading reasons of death in all parts of the world. Breast cancer is also responsible for the largest number of deaths among women population. Several studies confirmed that Bifidobacterium bifidum as a probiotic meaningfully inhibited breast cancer development. The present study aimed to investigate the effect of B. bifidum supernatant on the cell growth inhibition of the breast cancer 4T1 cell line, in vitro.
The present experimental work was conducted at Mazandaran University of Medical Sciences (MAZUMS), Sari, Iran. B. bifidum was cultured in the MRS broth at 37°C for 72 hours anaerobically and the B. bifidum supernatant (BS) was prepared by the freezing-thawing procedure. The cell growth inhibition of the probiotic strain was assessed using the MTT assay through breast cancer (4T1) cell line.
The results showed that the supernatant extracted from B.bifidum strain had good antiproliferative effects against 4T1 cancer cell line, compared to control group. The inhibitory effects growing with increased time.
B. bifidum supernatant could be considered as a as potential probiotic candidates in the treatment of breast cancer. However, further in vitro/in vivo studies are required to complement our initial findings.

The adjuvanticity potential of Lactobacillus casei was first suggested in an old survey. The present study was designed to investigate the efficacy of a new immunotherapy against breast cancer made by mixing an extract of heated 4T1 mammary carcinoma cell line and a heat-killed preparation of Lactobacillus casei.
Female BALB/c mice (6–8 weeks old, n=40) were challenged subcutaneously in the right flanks with 4T1 cells. When all the animals developed a palpable tumor, they were allocated to 4 equal groups and immunotherapy was initiated. The tumor-bearing mice in the experimental groups received the extract of heated 4T1 or heated Lactobacillus casei and/or a combination of both, twice at a 1-week interval. The mice in the control group received phosphate-buffered saline. One week after the last immunotherapy, one half of the mice were euthanized to determine the immune response profile. The remaining animals were kept until death occurred spontaneously.
The animals receiving the combined treatment significantly showed more favorable survival curves and slower rates of tumor development than the tumor-bearing mice receiving only the heated 4T1 and/or the negative control mice. The combined immunization significantly amplified the production of nitric oxide and the cytotoxicity of natural killer cells in the spleen cell culture of the tumor-bearing mice. Moreover, the combined immunotherapy significantly increased the secretion of IFN-γ and conversely diminished the secretion of IL-4 and TGF-β in the splenocyte population compared to the splenocytes from the other groups.
The combined immunotherapy with heated 4T1 cells and heated Lactobacillus casei conferred beneficial outcomes in our mouse model of breast cancer.

The 4T1 cells tumor growth and metastatic pattern in BALB/c mice very closely mimic human breast cancer. These herbal remedies used in traditional folk medicine have been the source of many medically beneficial drugs. The aim of the current study was to evaluate the anti-proliferative activity of the asafoetida, ginseng and fennel ethanolic extracts on mouse breast cancer 4T1 cell-line invitro. in this experimental study, Asafoetida, gensing and fennel were extracted; 4T1 mouse mammary tumor cell line were cultured in 48-well flat bottom plate in density of 50x103 per well in 100 µl RPMI1640 medium then different dilutions of each extract (25, 50, 100, 200, 500, and 2000 μg/ml) were added to cell culture. Cells then were incubated at 37 oC for 24 hours. After 24 h, cell Proliferation was determined by the BRDU assay. Mouse breast cancer 4T1 cell line was incubated with different doses of extracts. After 24h, cell proliferation was determined by the BrdU assay and the dose of 50μg/ml of fennel showed the best inhibitory effect. Anti-tumor activities of fennel, asafetida and ginseng ethanolic extracts may decrease proliferation activity of 4T1 cell line in vitro. The results suggested that fennel, asafetida and ginseng ethanolic extracts induce apoptosis and inhibit cell proliferation in vitro and fennel had the best anti proliferation effect.