جستجوی مقالات مرتبط با کلیدواژه « celosia leptostachya » در نشریات گروه « پزشکی »

Celosia leptostachya is classified under the Amaranthaceae family. C. leptostachya possesses numerous medicinal uses. Traditional medicine practitioners exploit C. leptostachya mainly on curing illnesses such as boils, fever, snake bites, scorpion stings, eye infections, wounds and pain and most notably, epilepsy.

This study was designed to evaluate the anticonvulsant properties of the ethyl acetate (EAF) and n-hexane fractions (NHF) of C. leptostachya leaf extract in mice and determine the most active extract between EAF and NHF.

The acute toxicity was carried out to determine lethal dose (LD50) using Lorke’s method. Pentylenetetrazole (PTZ), brucine and maximal electroshock (MES) were used to induce seizures in mice. We used Swinyard’s method of using an animal model of epilepsy. Thirty mice of both genders weighing 20-25 g were divided into five groups. Groups 1 and 5 are negative and positive controls, respectively. Groups 2, 3 and 4 were pre-treated with C. leptostachya extracts (100, 150 and 200 mg). Then, 30 minutes later, PTZ (90 mg/kg body weight) was administered. This method was repeated with brucine (110 mg/kg body weight). Regarding MES, after 30 minutes of administration of C. leptostachya leaf extract, an alternating current of 50 Hz and 35 mA was delivered to the animals in each group through ear-electrodes for 0.2 s.

Regarding MES, EAF (150 and 200 mg/kg) protected the animals against seizure, while NHF (150 and 200 mg/kg) could not prevent seizures. However, EAF and NHF (150 and 200 mg/kg) significantly decreased mean recovery time (P<0.05). In brucine-induced seizures, EAF (150 and 200 mg/kg) protected the mice against death and significantly (P<0.05) decreased mean recovery time. For PTZ-induced seizures, 200 mg/kg of EAF offered 100% protection against the mortality of mice and significantly reduced mean onset and recovering time (P<0.05). 

Based on the results, C. leptostachya has anticonvulsant properties and EAF extract has the highest potency.

Celosia leptostachya belongs to Amaranthaceae plant family. Its leaves are used traditionally in the treatment of conditions, such as convulsion, eye infection and most notably to cure snakebites. This study investigated the inhibiting effect of the extract of C. leptostachya leaves against cobra snake venom in mice.

We used 36 albino mice of mixed gender, weighing 20-25 g. They were divided into six groups of six rats each. Each rat was pre-treated with 0.4 mg/kg of cobra snake venom, and was subsequently given a graded dose of zero, 50, 100, 150, 200, or 250 mg/kg of the ethanol extract of C. leptostachya leaves. The animals were monitored for the survival rate. Various inhibition assays were performed to estimate the activities of acetyl cholinesterase, protease and adenosine triphosphatase of cobra venom, in the presence of 100-300 µg of the plant extract.

The extract inhibited the effects of cobra venom significantly after the intraperitoneal injection of the venom and extract at 20 minutes intervals. The results demonstrated that 100% of the mice survived if they received 100-250 mg/kg of the extract while only 83.3% survived with the extract at 50 mg/kg. The extract inhibited the venom’s acetyl cholinesterase, protease and adenosine triphosphatase. The inhibition occurred at higher percentages if the extract was given at 300 mg/kg. 

The plant extract significantly inhibited the snake venom, and its acetyl cholinesterase, protease and adenosine triphosphatase that mediated the venom’s toxicity.