جستجوی مقالات مرتبط با کلیدواژه « chromosome aberration » در نشریات گروه « پزشکی »

understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological malignancies are determinant factors in evaluation of radiation dose and its potential in harming the body. None the less early detection of chromosomal aberration (CA) is crucial in prognosis and selection of therapy for the people exposed to irradiations. The aim of this study was to explore a swift and accurate genetic test that identifies CAs in radiologist exposed to X-rays. In addition synergistic effect of other clastogens in irradiated workers was also evaluated.
Material and thirty four heparinized blood samples were obtained from radiology workers exposed to X-rays. Blood samples were cultured in RPMI 1640 and F-10 Medias with and without PHA stimulation. Lymphocytes were harvested, separated and arrested at metaphase and their chromosomes were analyzed by solid and G-Banding techniques. Lymphocytic CA was also analyzed through whole chromosome painting FISH.
of the 37 blood sample from workers, 60% had various structural aberrations in which both the frequency and type of CAs were intensified among tobacco smokers.
the results did not show any significant differences between the genders but other carcinogen like smoking can significantly increases the rate of CAs

The Picrorhiza kurroa rhizome has a long history of use in Indian Ayurvedic and Chinese system of medicine for the treatment of a wide spectrum of diseases. Today it is viewed as an important therapeutic target in both Western and Eastern medicinal systems. This work was aimed to study the clastogenic effect of Picrorhiza kurroa rhizome extract on cultured human peripheral blood lymphocytes.
Hydroalcoholic extract of rhizome was prepared and mammalian chromosomal aberration test was conducted using cultured human peripheral blood lymphocytes. The study was performed in two independent phases where the human peripheral blood lymphocytes were exposed to various of the extract in absence and presence of metabolic activation system for a continuous and short duration.
Picrorhiza kurroa rhizome extract did not induce chromosome aberration up to 2500 μg/mL in final culture concentration in the presence (1% v/v) and absence of metabolic activation.
Picrorhiza kurroa rhizome extract is completely safe to be used as a medicine since it manifest its healing effects without causing genotoxicity.

CT scan and nuclear medicine exams deliver a great part of medical exposures. This study examined professional radiation hazards in CT scan and nuclear medicine workers. In a cross sectional study 30 occupationally exposed workers and 7 controls (all from personnel of a laboratory) were selected. Physical dosimetry was performed for exposed workers. Blood samples were obtained from the experimental and control groups. Three culture mediums for each one were prepared in due to routine chromosome analysis using G-banding and solid stain. There were significant increased incidence of chromatid gap (ctg) and chromatid break (ctb) with mean±SD frequencies of 3±0.84 and 3.1±1.40 per 100 cells respectively in the nuclear medicine workers versus controls with mean±SD frequencies of 1.9±0.69 and 1.3±0.84 for ctg and ctb, respectively. Chromosome gaps (chrg) were higher significantly in the nuclear medicine population (2.47±0.91) than in controls (1.4±0.9) (p< 0.05). In CT scan group the ctg and ctb were increased with a mean±SD frequency of 2.7±0.79 and 2.6±0.91 per 100 cells respectively compared with control group. The mean±SD frequencies of the chrb were 2.0±0.75 and 0.86±0.690 per 100 cells for exposed workers and control group, respectively. This study showed chromosome aberrations in peripheral lymphocytes using solid stain method are reasonable biomarker reflecting personnel radiation damage.