جستجوی مقالات مرتبط با کلیدواژه "cytotoxic t lymphocyte" در نشریات گروه "پزشکی"

Granzyme-B is a serine proteinase expressed and released mainly by the cytotoxic T and NK cell. Granules intact Granzyme-B is directly delivered into the target cell, while extracellular Granzyme-B, released in serum leads to nonspecific cleavages of extracellular matrix molecules like vitronectin, collagen, TGF-β, IL-1 and invites systemic inflammation, tissue remodeling and fibrosis leading to the development of chronic renal allograft dysfunction.

We aimed to immunophenotype the Granzyme-B positive T-lymphocyte subset and Granzyme- B role in the development of chronic renal allograft dysfunction.

We have analyzed the Granzyme-B+CD8+T/CD8neg and Granzyme-B+CD3+/CD3neg cell subset by the flowcytometry and serum Granzyme-B level by the enzyme-linked immunosorbent assay.

We have found that the frequency of Granzyme-B+ CD8neg CD3+T cell, Granzyme-B+ CD8lowCD3+T cell and Granzyme-B+ CD8high CD3+T cell subset was significantly lower and serum Granzyme-B level was significantly higher in CAD group. The frequency of CD3+T, CD3neg lymphocyte, CD8neg CD3+T, CD8lowCD3+T, CD8high CD3+T, Granzyme-B+CD3negCD8neg lymphocyte was similar between the group. The frequency of CD3+CD8negGzm-B+ cell was negatively correlated with serum creatinine and CD3+CD8highGzm-B+ cell was negatively correlated with serum Granzyme-B level. Similarly, Serum Granzyme-B level was positively correlated with serum creatinine, urine proteinuria and negatively with eGFR.

The circulating frequency of Granzyme-B+ CD8neg CD3+T cell, Granzyme-B+ CD8lowCD3+T cell and Granzyme-B+ CD8high CD3+T cell subsets were significantly lower and serum Granzyme-B level was significantly higher in renal allograft recipients with CAD.

Adenovirus species B, C, D, and E are the most common causes of ocular manifestations caused by adenoviruses. FDA-approved treatment agents for adenovirus infections are not available. Cell-mediated immunity is the major protective mechanism versus human adenoviruses (HAdVs) infection and T cells specific for peptide epitopes from nonstructural proteins can prevent adenoviral dissemination. E1A CR2 region of HAdVs Epitopes predicted for reinforcing cytotoxic T lymphocytes (CTLs) in the EKC patients. Among human adenoviruses E1 protein, four distinct E1A regions had a significantly higher level of homology than the rest of E1A protein. E1A protein inhibits IFN signal transduction. Epitope-based vaccines are designed to have flexible and simple methods to synthesize a vaccine, using an adjuvant to trigger fast immune responses. CTL epitopes were applied to create a multiepitope vaccine. Conserve region1 (CR1) and CR3 have less antigenicity compared to CR2. Additionally, CR3 in HAdV-D8 contains three toxic areas. CR4 similar to the two regions CR1 and CR3 do not show acceptable antigenic properties.

Bioinformatics’ tools were used to predict, refine and validate the 3D structure of the construct. Effective binding was predicted by protein-protein docking of the epitope vaccine with MHC-I molecules and revealed the safety and efficacy of the predicted vaccine construct.

In silico analysis show that rising levels of cytotoxic CD8 + T cells, TH1 cells, macrophages, and neutrophils are linked to IFN-dominant TH1-type responses, which are detected in putative immune individuals.

Combined with 3D protein modeling, this study predicted the epitopes of E1A CR2 protein in HAdVs.