جستجوی مقالات مرتبط با کلیدواژه « entrotoxin b » در نشریات گروه « پزشکی »

Staphylococcus aureus bacteria causes many different diseases by secretion of various enterotoxins. Therefore, it is necessary to develop ways that facilitate the detection of enterotoxins. Nowadays, immunochemical methods which are based on monoclonal antibody technology are used. The heavy chain antibodies that are called VHH or Nano body were found in blood serum of the Camelidae family. The unique properties of this antibody such as their binding to small molecules like toxins make them attractive candidates for the development of immunodiagnostic tests. The present study was done to achieve a VHH molecules against Staphylococcus enterotoxin B.
Freighting phage library for isolate private Nano bodies against enterotoxin B was done in previous works. Next, pCANTAB 5E vector that consists VHH, extracted from E.coli bacteria strain xl1blue, and after doing PCR process with relative primers, sub cloning in pET21a() as an expression vector with cut sites NdeI and XhoI was done. Transformation in E.coli bacteria strain BL21(DE3) was done. Then, the cells effected with IPTG and producing time, and other terms were optimized. Finally, the expression of the protein with SDS-PAGE and western blot techniques was evaluated.
For proving cloning of nano body gene in pET21a () vector, nucleotide sequence of gene was analyzed, and transforming to E.coli bacteria strain BL21(DE3) was successful. After inspiration, active protein in cell was seen by SDS-PAGE technique and proved by western blot.
cloning, sub cloning, and nonabody expression were surveyed in this research. Production of this protein can help to develop new therapeutic methods and produce vaccine against enterotoxin B of Staphylococcus aureus

Staphylococcus species have higher abundance among isolated isolates from clinical samples. The spices of Staphylococcus aureus which produces enterotoxin A is more viable against host immune defence. The purpose of this study was to assess the relative abundance of entrotoxin A gene and its relation with antimicrobial resistance in isolates of Staphylococcus aureus isolated from clinical samples. Subjects and All of the isolates of Staphylococcus aureus from sample of patient sent to Golestan and Imam Khomeini hospital clinical labs، were tested biochemically in order to confirm the presence of Staphylococcus aureus strains. Antibiotic resistance of the isolates was assessed by disk diffusion method and PCR method for detection of entrotoxin A gene. Among 222 isolates of Staphylococcus aureus from different clinical samples، 102 (45. 94%) of these isolates had entrotoxin A gene. This isolates has been highly resistant to the antibiotics such as Ciprofloxacin، methicillin، gentamicin، erythromycin and clindamycin. The frequency of the Staphylococcus aureus isolates which produces enterotoxin A Gene in Golestan and Imam Khomeini hospitals was high (46. 3% and 45. 7% Respectively). This samples also have more resistance against antibiotics than the other which has not gene.