جستجوی مقالات مرتبط با کلیدواژه "escc" در نشریات گروه "پزشکی"

The lncRNA-LINC02389 gene is a long non-coding RNA, which has not been an assessed potential role in the pathogenesis of esophageal squamous cell carcinoma (ESCC).

The purpose of the present survey was to evaluate the lncRNA-LINC02389 gene expression variation in subjects with ESCC.

The present survey was a preliminary investigation performed on seventy-five paired paraffin blocks including tumorous and non-tumorous marginal tissues from subjects with ESCC. After extraction of totalRNAandcDNAsynthesis, the lncRNA-LINC02389 gene expression change was evaluated using quantitative Real-time PCR method.

Our data declared that the lncRNA-LINC02389 gene was significantly down-regulated in ESCC tissues compared to the marginal tissues (P < 0.05). In addition, the findings showed a significant association between the lncRNA-LINC02389 gene expression change and tumor differentiation grade (P = 0.003).

Our results proposed a possible carcinogenesis character of the lncRNA-LINC02389 gene and may be employed as a prognostic clinical significance in the progression of ESCC.

Esophageal squamous cell carcinoma (ESCC) is the most widespread type of esophageal cancer. LncRNA TUG1 was first identified in a genomic screening study for the treatment of taurine in retinal cells. This study aimed at analyzing TUG1 expression in ESCC tissues collected from an Iranian population of patients. Bioinformatics study was also conducted for better understanding the function of this lnc-RNA.

We examined the expression of TUG1 in 31 pairs of ESCC tissues and adjacent non-cancerous tissues by qRT-PCR. Bioinformatics analysis was conducted using various databases. Student’s t-test was performed using SPSS software (version 16.0) to evaluate the difference in TUG1 expression between ESCC and adjacent non-cancerous tissues.

TUG1 expression level in ESCC tissues was significantly higher than that in the adjacent non-cancerous tissues (P=0.04).

TUG1 is upregulated in ESCC, which may be related to history of smoking

Modeling cancer in vivo is a very important tool to investigate cancer pathogenesis and molecular mechanisms involved in cancer progression. Laboratory mice are the most common animal used for rebuilding human cancer in vivo. Cancer stem cells (CSCs) are the main reason of failure in cancer therapy because of tumor relapse and metastasis. Isolation of cancer stem cells helps us to study their function and behavior. In the current study we separate cancer stem-like cells using sphere formation assay then investigate their tumorigenicity in xenograft tumor model.

YM1 cancer cells were cultured in serum-free media (SFM) in low adherent culture dishes for enrichment of cancer stem cells. The resulting spheres containing cancer stem-like cells were dissociated into single cells and were injected into the dorsal flank of B6 nude mice.

A few days after injection, subcutaneous tumors formed. The growth curves of the resulting tumors were plotted using their weekly recorded lengths. The tumors' volume and weight were measured. The size of resulting tumors was appropriate to the number of cells injected. Pathological analysis confirmed esophageal origin of the resulting tumors.

Using laboratory mice models is a practical modeling system that provides us investigation of human tumors pathogenesis in vivo.

  Esophageal cancer (EC) is the second most common gastrointestinal cancer, and esophageal squamous cell carcinoma (ESCC) is the dominant type of EC in Iran. One of the most important challenges in cancer management is the early diagnosis. As tumor suppressors or oncogenes, long non-coding RNAs (lncRNAs) play a vital role in tumor initiation, progression and metastasis. Recent studies have reported dysregulated expression of lncRNAs in different tumor tissues including ESCC, which may indicate its prognostic or diagnostic potential in ESCC. In this study, we investigated expression pattern of lnc-POU3F3 in ESCC. We performed quantitative real-time polymerase chain reaction to assess lnc-POU3F3 expression pattern in ESCC blood samples from 32 patients with ESCC and age- and sex-matched healthy controls. Lnc-POU3F3 was significantly overexpressed in ESCC blood samples compared to normal samples. In addition, this overexpression had a significant correlation with family history (P=0.03) and TNM stage (P=0.02). According to the findings of the present study, lnc-POU3F3 may be used as a diagnostic biomarker for ESCC. However, studies with a larger sample size should be carried out to confirm this finding

HER-2/neu overexpression has been reported in various human cancers and identified as a significant predictor of poor survival. In this study HER-2/neu overexpression and its associations with clinicopathological characteristics were evaluated in patients with esophageal squamous cell carcinoma (ESCC).
This cross-sectional study was performed on 64 patients with histological diagnosis of primary ESCC who underwent surgery for curative treatment. Immunohistochemistry (IHC) was used to assess expression of HER-2/neu receptor in formalin-fixed paraffin-embedded tissue blocks.
The mean age of patients was 60.1 ± 1.28 years. The overall HER2 expression was observed in 51.5% of ESCC patients without considering IHC scores. HER2/neu overexpression (6%) was significantly associated with the tumor differentiation (P No significant correlations were found between HER2/neu overexpression and gender, age, tumor invasion, location of tumor, TNM stages and stage of tumor in patients with ESCC.

Human Cripto-1, a member of the EGF-CFC family, is involved in embryonic development, embryonic stem cell maintenance, and tumor progression. It also participates in multiple cell signaling pathways including Wnt, Notch, and TGF-β. Remarkably, it is expressed in cancer stem cell (CSC) compartments, boosting tumor cell migration, invasion, and angiogenesis. Although Cripto-1 is overexpressed in a variety of human malignant tumors, its expression in esophageal squamous cell carcinoma (ESCC) remains unclear. Our aim in this study was to evaluate the possible oncogenic role of Cripto-1 in ESCC progression and elucidate its association with clinicopathological parameters in patients.
In this study, Cripto-1 expression in 50 ESCC tissue samples was analyzed and compared to corresponding margin-normal esophageal tissues using quantitative real-time PCR.
Cripto-1 was overexpressed in nearly 40% of ESCC samples compared with normal tissue samples. Significant correlations were observed between Cripto-1 expression and tumor differentiation grade, progression stage, and location (p Our results indicate that overexpression of Cripto-1 is involved in the development of ESCC. Further assessment will be necessary to determine the role of Cripto-1 cross talk in ESCC tumorigenesis.

Esophageal squamous cell carcinoma (ESCC) is the second-most frequently diagnosed cancer in Northeast Iran, often diagnosed in advanced stages. No standard early diagnostic guideline has been proposed to date and current therapeutic modalities are not effective. Detection of tumor-specific biomarkers, which is the goal of this study, could prove useful in the diagnosis of ESCC. To better understand the gene expression profile of ESCC, we analyzed tumor samples and corresponding adjacent normal tissue from ESCC patients by Chemiluminescent Human Cancer GEArrays. Candidate genes were verified by real-time PCR. Out of 440 cancer-related genes included in the array, 71 were overexpressed compared to normal tissue, with significant differences in 11 genes. There were 108 genes underexpressed, with significant differences in 5 genes. Until now, the AP2M1, FTL, UBE2L6, HLA-C, and HSPA8 overexpressed genes and XRCC5, TP53I3 and RAP1A underexpressed genes were not reported in ESCC. We chose the MMP2, HLA-G, and XRCC5 markers from 58 Iranian ESCC patients to verify the expression validity by real-time PCR. The microarray results were confirmed with two-tailed significance levels of P = 0.003 (MMP2), P = 0.000 (HLA-G) and P = 0.002(XRCC5). Analysis performed for the candidate genes using GNCpro online software highlighted two pathways, an immuno-modulatory response and DNA replication and repair. We successfully performed and validated Chemiluminescent GEArray gene expression profiling in ESCC. Several biomarkers that might be related to tumorigenesis in ESCC were identified. Immuno-modulatory and DNA repair pathways could be used as targets to locate specific diagnostic, prognostic, and therapeutic biomarkers for ESCC.