جستجوی مقالات مرتبط با کلیدواژه « escherichia fergusonii » در نشریات گروه « پزشکی »

Several prominent bacterial species known to induce diarrhea in human hosts encompass Escherichia coli, Escherichia albertii, Escherichia fergusonii, and various Shigella spp. Given that these organisms contribute to the burden of food-borne illness, it is essential to rapidly and correctly identify them in a clinical laboratory or food microbiology unit to prevent their transmission and spread. These pathogens are often mistakenly identified because of their genetic and phenotypic similarities. Phenotypic tests are not highly discriminatory and are time-consuming. Whole-genome sequencing is expensive and unavailable in most clinical laboratories.

To simplify their rapid detection, we improved an available multiplex polymerase chain reaction (PCR) assay targeting three species-specific primers, including Eco (the main target for E. coli identification), Ealb (specific for E. albertii), and Efer (specific for E. fergusonii), by adding ipaH and lacY to additionally discriminate between the highly similar Shigella spp. and enteroinvasive E. coli (EIEC) organisms. Primers were tested on 65 defined isolates, including E. coli (n=29), Shigella spp. (n=26), E. fergusonii (n=1), E. albertii (n=1), and other Enterobacterales (n=8).

All examined E. coli yielded two amplicons of the expected size (Eco and lacY), except for EIEC, which had three bands (Eco, lacY, and ipaH). All Shigella spp. yielded two amplicons (Eco and ipaH). E. fergusonii had only one band (Efer), and E. albertii also yielded one band (Ealb). Other Enterobacterales that were tested for validation did not demonstrate a product, except for Klebsiella pneumoniae and Klebsiella oxytoca (both lacY).

The assay was shown to be a way forward for rapid, specific, and cost-effective primary discrimination of these important or emerging enteropathogens that can be used in clinical and research laboratories.

 Gram-negative bacilli are primarily responsible for the most common pediatric infections. Frequently, Escherichia fergusonii is identified as E. coli because of its close genetic proximity.

 We aimed at the isolation and identification of multi-resistant strains of E. fergusonii, affecting children under two months of age.

 Strains were isolated from infectious processes and were identified phenotypically and molecularly. The microdilution method (MicroScan, autoSCAN-4) and the disk diffusion method (modified Kirby Bauer) were used to analyze antibiotic susceptibility.

 Strains isolated were multi-resistant. Molecular identification provided the correct taxonomic assignment. Escherichia fergusonii strains were wrongly identified as E. coli with the phenotypic identification method. In addition, Pseudomonas aeruginosa and Klebsiella pneumoniae were identified. The best sensitivity results were obtained with Ceftazidime/avibactam and ceftolozane/tazobactam.

 We provided the first report of isolation and identification of multi-resistant E. fergusonii strains affecting children under two months of age in a neonatal intensive care unit.