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جستجوی مقالات مرتبط با کلیدواژه « fermented milk » در نشریات گروه « پزشکی »

  • علیرضا شهاب لواسانی*، محدثه زندی، لیلا ناطقی
    مقدمه

    کامبوچا یک نوشیدنی تقریبا ترش مزه و حاصل از تخمیر چای و شکر توسط نوعی مخمر به همراه باکتری است. گاها برخی از تولیدکننده ها به این نوشیدنی طعم دهنده نیز اضافه می کنند.این نوشیدنی به شکل طبیعی کمی گازدار است و سرشار از پروبیوتیک ها، آنتی اکسیدان ها، ویتامین های گروه ب و سایر مواد مغذی دیگر است.

    مواد و روش

    در این تحقیق تاثیر چای کامبوچا مخلوط شده با شیر تخمیری در غلظت‏های مختلف بر قابلیت زنده مانی سویه های لاکتوباسیلوس اسیدوفیلوس، بیفیدوباکتریوم بیفیدیوم و لاکتوباسیلوس رامنوسوس و خواص فیزیکوشیمیایی و حسی شیر تخمیری در طول 28 روز نگهداری در دمای1± 5درجه سانتیگراد مورد بررسی قرار گرفت. پس از کشت باکتری‏های پروبیوتیک مقدار cfu/ml 107 از آن ها به شیر پاستوریزه اضافه گردید. سپس چای کامبوچا در سطوح 0، 5، 10، 15 و 20 میلی لیتر در لیتر به مخلوط اضافه و در روزهای سوم، هفتم، چهاردهم و بیست و هشتم نگهداری در دمای 1± 5 درجه سانتیگراد آزمون‏های تعیین میزان پروتیین، اسیدیته، pH، اسید استیک، درصد ترکیبات آنتی اکسیدانی و میزان بقای باکتری‏ها برروی نمونه ها انجام شد.

    یافته ها

    نتایج نشان داد درصد پروتیین، اسیدیته، اسید استیک و فعالیت آنتی اکسیدانی با افزایش میزان کامبوچای در طول دوره نگهداری به طور میانگین به ترتیب 12/0، 02/0، 694/15و 16/0افزایش اما میزان pH و جمعیت باکتری‏های پروبیوتیک به طور معنی داری p<0.05 به ترتیب به میزان 79/0 و 046/0 سیکل لگاریتمی کاهش یافت. در طی دوره نگهداری میزان زنده مانی باکتری های پروبیوتیک به طور معنی داری کاهش یافت ولی بالاتر از حد استاندارد قابل قبول محصولات پروبیوتیک بود.

    نتیجه گیری

    یافته های این تحقیق نشان داد افزودن کامبوچای در سطح 5 درصد تیمار T1 در بهبود ویژگی‏های حسی تخمیری نقش موثری داشته و به عنوان تیمار برتر انتخاب شد.

    کلید واژگان: چای کامبوچا, شیر تخمیری, باکتری‏های پروبیوتیک, ویژگی های فیزیکوشیمیایی}
    A. Shahab Lavasani *, M. Zandi, L. Nateghi
    Introduction

    Nowadays, the selection of probiotics has an important role in the development of the country's food industry and the production of probiotic dairy products is the main indicator of this progress on the other hand, the quality, sensory and viability characteristics of probiotics in manufactured products are the main problems of most industrial factories.

    Material and Methods

    the effect of different concentrations of kombucha drink on the viability of Lactobacillus acidophilus, bifidobacterium bifidum, Lactobacillus Rhamnosus was studied and physicochemical and sensory characteristics of fermented milk were measured during 28 days of storing period at 5±1˚C. Different levels of Kombucha tea 0, 5, 10, 15 and 20 ml/L were added to probiotic fermented milk containing L. acidophilus, B. bifidum, L. Rhamnosus bacteria (Cfu/ml 107) .Viability rate of probiotic bacteria, pH, percentages of protein, acidity(Dornic), acetic acid, antioxidant compounds were measured during 3, 7, 14 and 28th of storing period at 4 ⸰C.

    Results

    Results showed that higher amounts of kombucha cause higher content of protein, acidity, acetic acid and antioxidant activity. The average amounts of theses parameters were respectivly 0.12,0.02, 15.694 and 0.16. however, pH and viability of probiotic bacteria significantly decreased during the storage period(p<0.05) and average amounts of these parameters were respectively0.79 and 0.046, However, the mentioned indices were higher than the acceptable standard of probiotic products.

    Conclusion

    the addition of kombucha up to 5% could be more effective in improving sensory characteristics. Hence, a treatment containing 5% of kambucha was selected as the best among others.

    Keywords: Kombucha, Fermented Milk, Probiotic Bacteria, Physicochemical Characteristics}
  • فائزه شیرخان، سعید میردامادی*، مهتا میرزایی، بهروز اکبری آدرگانی، نیکو نصوحی
    مقدمه

    با افزایش توجه مصرف کنندگان به محصولات غذایی با خواص حسی مناسب و دارای اثرات سلامت بخشی، تمایل به تولید محصولات لبنی فراسودمند در سال های اخیر افزایش یافته است.

    مواد و روش ها

    در مطالعه حاضر برای تولید شیر تخمیری فراسودمند ابتدا اثر سویه های لاکتوباسیلوس دلبروکی، لاکتوباسیلوس هلوتیکوس جدا شده از ماست سنتی ایران و کشت توام دو سویه بر فعالیت ضد دیابتی شیر تخمیری بر اساس فعالیت مهارکنندگی آنزیم های آلفا آمیلاز و آلفا گلوکوزیداز بررسی شد. سپس اینولین به عنوان یک پری بیوتیک مناسب و کاربردی، به میزان 1 درصد به شیر تخمیری حاصل از ترکیب دو سویه اضافه و ویژگی های فیزیکوشیمیایی، ضد دیابتی، آنتی اکسیدانی، میزان پروتیولیز و جمعیت باکتری ها در طی 19 روز نگهداری در دمای 4 درجه سانتی گراد مورد ارزیابی قرار گرفت.

    یافته ها

    نتایج نشان داد شیر تخمیر شده بوسیله کشت توام دو سویه باکتریایی، فعالیت مهارکنندگی بیشتری بر آنزیم های آلفا آمیلاز (%45) و آلفا گلوکوزیداز (35%) نسبت به سویه های تکی لاکتوباسیلوس دلبروکی و لاکتوباسیلوس هلوتیکوس دارد (05/0<p) و افزودن اینولین باعث افزایش فعالیت مهارکنندگی آنزیم آلفا آمیلاز (20%) و آلفا گلوکوزیداز (33%) در زمان پایان تخمیر شد. ترکیب دو سویه باکتریایی و اینولین در طول دوره نگهداری در دمای 4 درجه سانتی گراد بطور معنی داری باعث افزایش جمعیت باکتری ها، اسیدیته، ویسکوزیته و کاهش معنی دار pH و میزان پروتیولیز شیر تخمیری گردید (05/0<p). با این وجود در طول این زمان کاهش فعالیت آنتی اکسیدانی و ضد دیابتی مشاهده گردید.

    نتیجه گیری

    در مجموع نتایج نشان دهنده پتانسیل مناسب دو سویه باکتری به عنوان مایه تولید محصول تخمیری همراه با اینولین برای تولید محصول غذایی فراسومند بود.

    کلید واژگان: اینولین, دیابت, زمان نگهداری, سویه های بومی, شیر تخمیری, غذاهای فراسودمند}
    Faezeh Shirkhan, Saeed Mirdamadi *, Mahta Mirzaei, Behrouz Akbari-Adergani, Nikoo Nasoohi
    Introduction

    The inclination to manufacture beneficial dairy products has increased in recent years, as consumers pay more attention to food products with adequate sensory qualities and health benefits.

    Materials and Methods

    The current study looked first at the anti-diabetic efficacy of milk fermented by Lactobacillus delbrueckii, and Lactobacillus helveticus strains isolated from traditional yogurt as well as co-culture of two-strain based on the inhibitory activity of alpha-amylase and alpha-glucosidase enzymes for the production of functional fermented milk. Then, added 1% inulin as an appropriate and applied prebiotic to fermented milk resulting from the combination of two strains and evaluated physicochemical, anti-diabetic, anti-oxidant, proteolysis rate, and population of bacteria during 19 days at 4°C.

    Results

    The results indicated that milk fermented with two-strain bacterial intercropping had stronger inhibitory activity on alpha-amylase (45%) and alpha-glucosidase (35%) than single strains of Lactobacillus delbrueckii and Lactobacillus helveticus (p<0.05). The addition of inulin increased the inhibitory activity of alpha-amylase (20%) and alpha-glucosidase (33%) at the end of fermentation, respectively. During storage at 4°C, the composition two bacterial strains and inuline significantly increased the population of bacteria, acidity and viscosity and significantly reduced the pH and proteolysis rate of fermented milk. However, there was a decrease in antioxidant and antidiabetic activities.

    Conclusion

    The results revealed that both bacterial strains in combination with inulin have the capacity to produce functional food.

    Keywords: Inulin, Diabetes, Storage time, Native strains, Fermented Milk, Functional foods}
  • Reza Zadeh Dabbagh, Abdolazim Behfar, Marjan Javadi, Naji Saiahi, Maryam Alborzi, Maryam Seyedtabib, Seyyed MohammadAli Noori*
    Background

    A lot of people consume traditional foods throughout the world, however there are concerns for their safety. The purpose of the current study is to evaluate the safety of traditional doogh (a traditional fermented dairy beverage) with respect to microbiological, chemical, and toxicological aspects.

    Methods

    89 samples of traditional doogh from Khuzestan province, Iran, were analysed according to microbiological (Staphylococcus aureus, Echerichia coli, coliforms, mold and yeast), chemical (pH, total solid, fat, and salt content), and toxicological (aflatoxin M1, potassium sorbate, sodium benzoate, and natamycin) aspects.

    Results

    Results showed that 89.88% of samples would be rejected according to the guidelines specified by the Institute of Standards and Industrial Research of Iran (ISIRI). 78 sample had higher mold and yeast than standard. The salt content of five out of 89 samples were higher than the established Iranian standard.

    Conclusion

    The most challenging problem was mold and yeast contamination, indicating poor hygienic manufacturing conditions. However, the unfavorable conditions for microbial growth such as low pH may prevent the toxic metabolites formation, and therefore, consuming it presents no major concerns to public health. Nonetheless, the authors suggest that efforts should be made to improve hygienic conditions in traditional doogh manufacturing environments.

    Keywords: Dairy microbiology, Doogh, Fermented milk, ELISA, HPLC}
  • Fahime Ajam, Hadi Koohsari *
    Antibacterial activities of kefir beverage are attributed to kefiran polysaccharide. The aim of this study was to investigate the effect of type of milk, fermentation time, temperature and stirring conditions on antibacterial activity of extracted kefiran from kefir grains. Kefir grains were added to full-fat and non-fat milk. Fermentation was carried out at 25°C and 37°C under stirred and non-stirred conditions. After 24, 48, 72 and 120 hours, the grains were separated from kefir extract and kefiran was extracted. The antibacterial activity of extracted kefiran was carried out by well method. Fermentation time had a significant effect on the inhibition zone of all tested bacteria. Milk type had significant effect on antibacterial activity of kefiran samples against tested bacteria except S. dysenteriae. Fermentation temperature had a significant effect on the inhibition zone of E. coli and B. cereus, but stirring conditions only had significant effect on inhibition zone of B. cereus. Considering all factors and their interactions, in order to extraction of kefiran with higher antibacterial activity is recommended, fermentation in full-fat milk. Also, to achieve higher antibacterial activity against B. cereus, E. coli, S. dysenteriae, and S. aureus, is recommended fermentation time for 24, 48, 48 and 120 hours, respectively. Fermentation at 37°C compared to 25°C and under stirred compared to non-stirred conditions did not create noticeable differences in the antibacterial activity of extracted kefiran from kefir grains. Fermentation conditions had significant effect on antibacterial activity of extracted kefiran from kefir grains.
    Keywords: Kefiran, Kefir Grains, Antibacterial activity, Fermentation conditions, Fermented milk}
  • Fenny Amilia Mahara, Lilis Nuraida *, Hanifah Lioe
    Folate, also known as vitamin B9, is essential in cell metabolism and very important especially for pregnant women and lactating mothers. Natural folate is available in food but it is very unstable. Synthetic folate is generally used as an alternative to meet daily needs due to its stability, even though it has a negative effect causing a variety of metabolic disorders. Some lactic acid bacteria have been reported as being able to synthesize natural folate during the fermentation process. Lactic acid bacteria are the main microorganisms for lactic fermentation such as fermented milk, fruits, and vegetables. Milk is the most nutritious food and contains folate-binding protein, hence it is considered the ideal fermentation medium to increase folate stability during storage. Fermentation of milk with folate-producing lactic acid bacteria can be used as a technique to produce natural folate-rich fermented foods as an attempt to prevent folate deficiency without side effects to the consumers.
    Keywords: Lactic acid bacteria, Fermentation, Fermented Milk, Folate, Folate-Rich Fermented Food}
  • Z. Barzegar, M. Jahadi *, M. A. Hanifpour

    Nowadays, milk tablets are considered favorable due to several advantages, including good taste and chewing properties, ease of transport, increased bioavailability, ease of storage, stability of active ingredients and improved functional properties.This study seeks to investigate the characteristics of fermented milk tablets in relation to its use as a beverage producing tablet. The effects of effervescent agent contents (0, 20, 40, 50, 60 %) (citric acid, tartaric acid and sodium bicarbonate) on physical and sensory properties of fermented milk tablets were evaluated. Physical properties, including weight and diameter of the tablet containing different percentages of effervescent agent (i.e. 0-60 %) did not differ significantly (P≤0.05). Increasing the level of the effervescent agent (i.e. 0-60%) led to hardness reduction, as well as the friability value and dissolution time of this tablet in water. Regarding the sensory properties of the fermented milky drink produced by tablets, the appearance, mouth feel and flavor acceptability of fermented milk tablets containing 40 % effervescent agent had the highest significant score (P≤0.05).

    Keywords: Effervescent Agents, Fermented Milk, Physical Properties, Tablet}
  • N. Soleymanzadeh, S. Mirdamadi *, M. Kianirad

    Lactic acid bacteria, including lactobacilli, enterococci, leuconostoc and weissella species isolated from Iranian traditional fermented camel milk (Chal) were assessed for the incidence of virulence determinants (gelE, efaAfm, efaAfs, ace, espfs, cylM, cylA and cylB), sensitivity to various antibiotics and virulence phenotypes. The incidence of virulence genes was determined by polymerase chain reaction and antibiotic susceptibility was tested by disk diffusion method. The results of this study indicated that all of the strains harbor at least two or more of the virulence genes. The most frequent virulence genes detected among tested strains were cylB, gelE and efaAfs. All strains showed no β-hemolysis while tyrosine decarboxylase activity and gelatinase production were observed in enterococcus and leuconostoc strains. Majority of strains were resistant to Polymyxin B and kanamycin. Lactobacillus strains including L. paraplantarum, L. kefiri, L. paracasei, L. plantarum and Weissella cibaria were resistant to both vancomycin and kanamycin. The possibility of transferring the antibiotic resistance and virulence genes to other starter and commensal strains makes the usage of these strains in food and dairy products controversial without required safety assessments.

    Keywords: Enterococcus faecium, Fermented Milk, Lactic Acid Bacteria, Safety Assessment, Virulence Determinants}
  • Tayyebeh Sarlak, Maryam Moslehisahd *, Behrouz Akbari, Adergani, Maryam Salami
    The aim of this study was to investigate the effects of starters and storage temperature 4 ºC, 25 ºC) on microbiological and physicochemical properties, volatile compounds and sensory evaluation of kefir. Kefirs produced by KFA and Chr. Hansen starters were stored at 4 ºC and 25 ºC for 40 days. pH and acidity at 4 ºC did not change (p ≥ 0.05), while at 25 ºC pH and total solid decreased as well as acidity. Concentrations of acetaldehyde and ethanol increased (p
    Keywords: Fermented milk, Kefir, Functional properties, Microbiological analysis, Sensory evaluation}
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