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عضویت

جستجوی مقالات مرتبط با کلیدواژه « germination » در نشریات گروه « پزشکی »

  • Doris Anaemene*, Ngozi Arisa, Damilola Adeoti

    Bambara nut, an underutilized legume is rich in protein and can improve the nutritional quality of snacks. This study evaluates the nutritional and sensory properties of muffins produced from germinated maize and Bambara groundnut flours.

    Methods

    Maize and Bambara groundnut seeds were germinated (72 hours), oven-dried and milled. The composite flours from the germinated flours were combined with soya oil, tiger-nut milk, date and carrot powder to produce four samples of muffins. The samples were chemically analyzed for proximate, mineral, beta-carotene, and anti-nutrient contents. Then, sensory evaluation was carried out among 20 mothers (20-30) using a 5 hedonic scale. ANOVA and Duncan Multiple Range Test were also used to analyze the data.

    Results

    Protein, fiber and carbohydrate contents of the muffins ranged from 7.63-9.23%, 0.05-0.08% and 70.63-71.55%, respectively. It was found that germination significantly increased protein and iron content of muffins but reduced zinc, calcium, and beta-carotene contents (P<0.001). The muffins contributed above 22% of the RDA regarding energy for children. Muffins produced from the blends provided higher level of protein, calcium, iron and beta-carotene compared to those made from whole wheat flour. There was a significant reduction in anti-nutrient contents of the muffins and germinated flours. The muffin produced from the 80% maize and 20% Bambara nut flour was rated best in terms of nutritional value, taste, and texture.

    Conclusion

    Bambara nut flours could be used to produce nutritious snacks which would contribute to daily energy and protein needs of children

    Keywords: Food Processing, Germination, Legume, Infant Food}
  • Ali Abdulrahman Fadhil*, Sajeda Y. Swaid, Samar Jasim Mohammed, Aswan Al Abboodi

    Soil salinity is an escalating problem that significantly reduces crop yield, particularly in regions with intensive agriculture or poor irrigation practices. This study aimed to assess the impact of salinity on the germination and early growth parameters of four tomato (Solanum lycopersicum) cultivars: 'Sakata', 'US Agriseed', 'Rossen B.V.', and 'Supermarmance'.

    Methods

    The experiment was conducted under controlled greenhouse conditions with a randomized complete block design. Seeds were exposed to five salinity treatments (0, 4, 6, 8, and 10 ds m 1), and growth parameters including germination rate, seedling length, leaf number, and wet and dry weights were measured over a two week period. The results demonstrated that increasing salinity levels had a significant inhibitory effect on all measured growth parameters across all cultivars. Germination rates and seedling vigor decreased with increasing salinity, and a complete inhibition of growth was observed at the highest salinity levels (EC 8 and EC 10). However, variability among cultivars indicated differential salinity tolerance, with 'US Agriseed' displaying relatively better performance under saline conditions. The study provides clear evidence that salinity levels as low as 4 dS m 1 can adversely affect the germination and seedling growth of tomato plants. The findings highlight the critical need for developing salinity management strategies and breeding programs to improve salinity tolerance in tomatoes, which could significantly mitigate the impact of salinity stress on crop productivity.

    Keywords: Salinity stress, Tomato, Germination, Seedling growth, Soil salinity, Plant tolerance}
  • Samar Jasim Mohammed, Rosimah Nulit, MohamedIsmail Abdel-Ghaffar Fayed *

    In constructing a liquid enhancer, 300 mM NaCl was primed for 72 hours on sterile MTi2 seeds., after that, deionized water was used as a control, and SA alone (salicylic acid) (0.25, 0.5, 0.75, 1 mM) and only KCl (Potassium chloride) (10, 20, 30, 40, and 50 mM) were applied. As previously stated, germination parameters were computed. After that, the appropriate ratio of KCl to SA was combined, and its efficacy as a germination activator on the Salt-stressed MTi2 seeds was examined. The data analysis software used was SPSS Windows version 22. To find the significant difference between treatments, data are first subjected to a two-way ANOVA with p≤0.05 confidence level. For purpose of comparing means, DMRT is next applied at a p≤0.05. According to the results, the best concentrations for boosting the germination and early growth of MTi2 seedlings in comparison to the control treatment were found to be 20–30 mM KCl and 0.5–0.75 mM SA. Furthermore, MTi2 seedling germination and early growth were more than 1x higher when the best concentrations of KCl (20-30 mM) and SA (0.5-0.75 mM) were combined. Salicylic acid (SA) and low levels of KCl applied to salt-stressed MTi2 seeds can help reduce the negative effects of salinity stress and enhance the percentage, rate, vigour, length, and biomass of the seedlings that germinate. Conclusion, Salt-stressed MTi2 seeds can benefit from an enhancer that increases germKeywordsination at low concentrations of KCl and SA.

    Keywords: Germination, salinity stress, seedlings, salicylic acid, Potassium chloride}
  • Hassan Hashemi, Nasim Salehi, Saeed Rajabi*, Kaan Isinkaralar
    Background

    Leachate, a highly contaminated liquid, is produced by separating wastes and introducing moisture into the waste layers. Biological toxicity evaluation is a method that may be used to analyze the toxicity of leachate to organisms and plants. Sorghum bicolor was employed in this study to evaluate the acute toxicity of raw leachate and landfill leachate.

    Methods

    Leachate was collected from different locations within the refuse that was collected and mixed in a sampling container. The physiochemical characteristics of the leachate were analyzed in both raw leachate and landfill leachate. Germination rate, root weight, and root length were measured 24, 48, and 72 hours after planting for leachate dilutions of 25%, 50%, 75%, and 100%, respectively.

    Results

    Raw leachate had greater concentrations of metals (Fe, Zn, Cu, Mn, Cr, Cd, and Pb), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), nitrate, ammonia, and phosphate, as well as a more acidic pH (< 6), as compared to landfill leachate. The results showed that landfill leachate had a lower potential for toxicity than raw leachate, with seed-germination rates of 0.1 and 0 in the presence of 75% landfill leachate after 48 and 72 hours, respectively, as opposed to 0.3 and 0.1 in the presence of raw leachate.

    Conclusion

    The findings show that raw leachate can include higher concentrations of metals and organic compounds, which can be one of the causes of Sorghum seed phytotoxicity. Waste leachate management is one of the most important pillars of environmental protection, and it should be taken into consideration by the right authorities.

    Keywords: Sorghum, Germination, Leachate, Landfill, Phytotoxicity}
  • Jasper O.G, Elechi*, Juliana I, Sule

    Plant foods contain almost all of the essential mineral and organic nutrients for human, as well as several unique organic phytochemicals that have been linked to the promotion of good health. This study investigated the influence of traditional food processing systems on the food safety, chemical compositions, and functional properties of pumpkin (Cucurbita pepo L) seed flour.

    Methods

    Fresh mature pumpkin fruits were procured from the laboratory. The pumpkin seed was divided into three different portions and subjected to traditional sand toasting, germination, and processing into flour. The samples were analyzed for proximate composition, minerals, heavy metals, vitamins, functional properties, and microbial safety.

    Results

    The result of proximate composition analyses revealed that raw and traditionally processed pumpkin seed flour had 33.75-39.63% protein, 43.65-36.36% fat, 7.38-5.89% moisture, 4.85-6.04% ash, 1.89-2.25% fiber, and 5.94-14.49% carbohydrate. The mineral and heavy metal content were in the range of 7.17-4.13 mg/100 Iron, 117.88-221.55 mg/100 g Potassium, 7.41-9.01 mg/100 g Calcium, 45.67-55.65 mg/100 g Phosphorus, 0.89-4.67 mg/100 g Iodine, and 7.39-6.33 mg/100 g Zinc. The toxic/deleterious elements values for Hg, Pb, As, and Cd were 0.018-0.004 mg/100 g; 0.021–0.062 mg/100 g, 0.013–0.092 mg/100 g, and 0.0011-0.0051 mg/100 g, respectively.  Cyanide, Nitrate, Phytate, and Oxalate contents of the pumpkin seed flour were 0.72-0.32 mg/100 g, 2.77–1.00 mg/100 g, 35.56–14.56 mg/100 g, and 0.073–0.030 mg/100 g, respectively. An appreciable amount of vitamins (Beta-carotene, vitamin C, and thiamine) were recorded with excellent functional properties. The microbial count was within the limit of national and international standards showing the processed pumpkin seed flour to be safe. 

    Conclusion

    It can be concluded that subjecting pumpkin seeds to traditional processing methods enhanced their nutritional properties and food safety quality. Therefore, adopting this traditional processing approach can be used in rural community nutrition and nutrition in emergency feeding programs in developing countries.

    Keywords: Germination, Toasting, Traditional food system, Ant-nutritional factors, Food safety}
  • Abdelghafour Doghbage*, Hassen Boukerker, Fathi Abdellatif Belhouadjeb, Bouzid Nedjimi
    Background

    The problem of soil contamination is a major concern of emerging countries. Heavy metals such as lead, cadmium, copper, zinc, and mercury cannot be biodegraded, and therefore, persist in the environment for long periods. It has become essential to develop efficient techniques for decontamination of polluted soils. One of these techniques, phytoremediation, is economically viable and compatible with environmental preservation policies. The application of phytoremediation in salty areas requires the use of plants that are able to tolerate heavy metals and high soil salinity. Atriplex halimus is one of the species with a great purification potential, which is a spontaneous halophyte species, and endowed with a fairly important aerial and root biomass. This study aimed to determine the effect of cadmium sulfate (CdSO4) on the germination and growth of A. halimus, and to assess its ability to accumulate cadmium and proline.

    Methods

    This is an experimental study of the tolerance of A. halimus to increasing concentrations of CdSO4 by an in vivo test. This study aimed to assess the degree of tolerance of this perennial species towards different concentrations of CdSO4.

    Results

    The results show a non-significant effect of CdSO4 on the seed germination rate and growth of the aerial and root parts. However, a significant accumulation of proline was observed especially in the aerial part, whereas the highest cadmium content was recorded at the roots.

    Conclusion

    According to the results, this species can be used in phytoremediation to decontaminate soils contaminated by heavy metals.

    Keywords: Heavy metals, Phytoremediation, Atriplex, Germination, Cadmium, Proline}
  • محسن فریدونی، حسین حاجی آقاعلیزاده*
    مقدمه
    امروزه فنآوری های مختلفی در حوزه کشاورزی مورد استفاده قرار می گیرد. فناوری پلاسما یکی از روش هایی است که می تواند جوانه زنی را بدون صدمه جانبی به بذر بهبود بخشد. در این مقاله، هدف بررسی تاثیر پلاسمای سرد بر پایه تخلیه کرونا بر جوانه زنی ارقام نخود عادل، منصور و آزاد است.
    مواد و روش ها
    نمونه های پیش تیمار با پلاسمای سرد به مدت 30 و 60 ثانیه به همراه نمونه های شاهد در شرایط یکسان برای جوانه زنی و ارزیابی تغییرات رنگ مورد بررسی قرار گرفتند.
    یافته ها
    نتایج نشان داد بذرهای ارقام عادل و آزاد در تیماردهی 30 ثانیه و رقم منصور در تیماردهی 60 ثانیه با پلاسمای سرد، دارای طول ریشه بیشتری نسبت به نمونه های شاهد بودند. پس از تجزیه و تحلیل آماری، مشخص شد که طول ریشه در شرایط یکسان، در مدت زمان های تیماردهی با پلاسمای سرد، دارای اختلاف معنی داری در سطح 5% نسبت به شاهد می باشد. از طرفی با بررسی مولفه های شاخص رنگ در نمونه های مورد آزمایش، تغییر معنی داری در نمونه ها مشاهده نگردید. بیشترین تغییرات در نسبت شاخص اختلاف رنگ در نمونه های رقم عادل دارای مواجهه 60 ثانیه با پلاسمای سرد نسبت به نمونه های دارای 30 ثانیه مواجهه به میزان 1/48% محاسبه گردید. همچنین برای مشاهده تغییرات رنگ نمونه ها در اثر پلاسما، مدل درجه سوم در محیط فازی عصبی در 8 ناحیه طراحی گردید که تغییرات جزیی رنگ را پس از یادگیری، با خطای کمتر از 0/01 نمایش داد.
    نتیجه گیری
    نتایج نشان داد استفاده از تیمار پلاسمای سرد می تواند بدون ایجاد عوارض جانبی بر جوانه زنی و بهبود رشد اولیه بذر نخود موثر باشد.
    کلید واژگان: پلاسمای سرد, رنگ, نخود, جوانه زنی, مدل سازی}
    Mohsen Fereydooni, Hossein Haji Agha Alizade *
    Introduction
    Today, various technologies are used in the field of agriculture. Plasma technology is one of methods that can improve germination without harming the seeds. In this paper, the aim is to investigate the effect of cold plasma based on corona discharge on germination of Adel, Mansur and Azad chickpea varieties.
    Results
    The results showed that the seeds of Adel and Azad varieties in 30 seconds cold plasma treatment and Mansour variety in 60-seconds cold plasma treatment, had longer root length than the control samples. After statistical analysis, it was found that root length under the same conditions, during cold plasma treatment periods, had a significant difference at the level of 5% compared to the control. On the other hand, by examining the components of color index in the tested samples, no significant change was observed in the samples. The most changes in the ratio of color difference index in samples of Adel variety with 60 seconds of exposure to cold plasma compared to samples with 30 seconds of exposure were calculated to be 1.48%. Also, to observe the color changes of the samples due to plasma, a third degree model was designed in a neural fuzzy environment in 8 regions, which showed minor color changes after learning, with an error of less than 0.01.
    Conclusion
    The results showed that the use of cold plasma treatment can be effective on germination and improve the initial growth of chickpea seeds without causing side effects.
    Keywords: Cold Plasma, Germination, Color, Modeling, Chickpea}
  • زهرا ترسلی، محمدرضا لبافی، فرزانه جوکار شوریجه*
    مقدمه

    گیاهان دارویی حاوی ترکیبات فیتوشیمیایی که اثرات مهاری بر عوامل بیماری زای گیاهی دارند. علف های هرز با محصولات اصلی رقابت می کنند و رشد، عملکرد و کیفیت محصولات کشاورزی را تا حدی کاهش می دهند. یکی از رایج ترین روش های کاهش آسیب علف های هرز استفاده از علف کش های شیمیایی است. با توجه به عوارض جانبی که علف کش های شیمیایی روی سلامت انسان و محیط زیست دارند، نیاز به جایگزینی علف کش های زیست سازگار و کم خطر وجود دارد.

    هدف

    در این مطالعه، خواص آللوپاتیک اسانس آنقوزه (Ferula assa-foetida L.) و روغن کرچک (Ricinus communis L.) به عنوان علف کش گیاهی برای جلوگیری از جوانه زنی بذرهای علف هرز تاج خروس (Amaranthus retroflexus L.) مورد بررسی قرار گرفته است.

    روش بررسی

    در این راستا، یک فرمولاسیون گیاهی بر پایه اسانس آنقوزه و روغن کرچک تهیه شد و تاثیر آن بر جوانه‎زنی بذر علف هرز تاج خروس در شرایط آزمایشگاهی مورد بررسی قرار گرفت. ترکیب شیمیایی فرمولاسیون گیاهی توسط GC/MS آنالیز شد.

    نتایج

    نتایج نشان داد که این ترکیب گیاهی در غلظت 0/75 و 1 درصد از جوانه زنی بذر علف های هرز حدود 70 درصد جلوگیری می کند. ترکیبات اصلی  اسانس آنقوزه و روغن کرچک به ترتیب  (E)-1- پروپنیل سکو بوتیل دی سولفید (43/9 درصد) و ریسینولییک اسید متیل استر (58/1 درصد) بودند.

    نتیجه گیری

    مشخص شد که فرمولاسیون گیاهی مورد مطالعه دارای خاصیت علف کشی است. بنابراین، برای دستیابی به نتایج امیدوارکننده به منظور جایگزینی علف کش های شیمیایی با علف کش های گیاهی، تحقیقات بیشتری مورد نیاز است.

    کلید واژگان: علف کش, اسانس, جوانه زنی, زیست سازگار, ایمن}
    Zahra Tarassoli, Mohammadreza Labbafi, Farzaneh Jokar Shoorijeh*
    Background

    Medicinal plant contain phytochemicals which have inhibitory effects on plant pathogens. Weeds compete with the main crops and reduce the growth, yield, and quality of agricultural products to some extent. One of the most common methods of reducing weed damage is the use of chemical herbicides. Due to the side effects that chemical herbicides on human health and the environment, there is a need to replace biocompatible and low-risk herbicides.

    Objective

    In this study, the allelopathic properties of the Ferula assa-foetida L. essential oil and castor oil (Ricinus communis L.) have been investigated as a botanical herbicide to prevent germination of redroot pigweed seeds (Amaranthus retroflexus L.).

    Methods

    In this regard, an herbal formulation based on Ferula assa-foetida essential oil and castor oil was prepared and its effect on the germination of redroot pigweed seeds was studies in laboratory conditions. The chemical composition of the herbal formulation was analyzed by GC/MS.

    Results

    The results showed that this herbal formulation in concentration 0.75 % and 1 % inhibits the germination of weed seeds about 70 %. The main constituents of Ferula assa-foetida and Ricinus communis were (E)-1-propenyl sec-butyl disulfide (43.9 %) and ricinoleic acid methyl ester (58.1 %), respectively.

    Conclusion

    It was found that the studied botanical formulation has herbicidal properties. Therefore, more research is needed to achieve promising results in order to replace chemical herbicides with botanical herbicides.

    Keywords: Herbicide, Essential oil, Germination, Biocompatible, Safe}
  • A. Yasser, K. Rezaei *, M. Salami

    Legumes and pulses have high nutritive values and functional properties that exert positive effects on health and nutrition. This study developed a novel complementary baby food using germinated mung bean and cowpea as sources of extra nutrients supplemented to the infants (aging 6-12 months). The carbohydrate contents of mung bean and cowpea showed 64.3 and 64.0% reduction, respectively, during 48 h of germination. However, phosphorous and zinc contents and antioxidant activities of mung bean and cowpea and the iron content of cowpea increased over the same period of germination. Five baby food formulations were prepared and evaluated according to a standard formula for a commercial baby food which was also used as the control. The finalized formula had higher protein (22.4%), calcium (6,100 mg kg‐1), phosphorus (5,133 mg kg ‐1) and vitamin D (329 IU in 100 g) contents but lower contents of iron (55.5 mg kg ‐1), vitamin C as ascorbic acid (0.1 mg in 100 g) and vitamin B12 (1.2 mg in 100 g) than the control. A finalized formula with good appearance, flavor and taste as well as an overall general acceptance was obtained that can be used to fight mal-nutritional issues in certain developing countries.

    Keywords: Antioxidant Activities, Complementary Baby Food, Cowpea, Germination, Mung Bean}
  • Pegah Sayyad, Amin, Ali, Reza Shahsavar *
    Persimmon (Diospyros kaki) is an important subtropical, monoecious, dioeocious and polygamous tree which belongs to Ebenaceae family. Because of low total seed and low viability seed in persimmon, grafting on seedling rootstock is generally used for its propagation. The common rootstock is Diospyros lotus, but its seeds have long dormancy. So, the study was carried out to investigate the effects of stratification (0, 25, 50 and 70 days) at 4-70C, scarification with (97%) sulphuric acid (0, 2.5, 5 and7.5 minute) and GA3 (0, 250, 500 and 750 mgL-1) on seed germination of D. lotus. Results showed that the most germination rate was observed in GA3 at 250 mgL-1. Stratification for 70 days had the most germination percent. The most germination uniformity was observed in GA3 at 500 mgL-1. In scarification plus stratification, the germination percent was lower than control and stratification.
    Keywords: Germination, Stratification, Scarification, Diospyros lotus}
  • Ali Fallah, Azadeh Mohammad-Hasani, Abasalt Hosseinzadeh Colagar *
    Zinc (Zn) is the second most abundant trace element in human, which cant be stored in the body, thus regular dietary intake is required. This review explained the physiological and pathogenesis roles of zinc in men's health and its potentials in germination, quality of sperm, and fertilization. Our investigation showed that Zn contained many unique properties in human, especially males. The antioxidant quality is one of them. Also, the increased reactive oxygen species levels in the seminal plasma of men who are both infertile and smokers influence the Zn content of seminal plasma in a way that physiology of spermatozoa can be affected as well. Moreover, Zn acts as a toxic repercussionagainst heavy metals and cigarette inflammatory agents. Zinc as a hormone balancer helps hormones such as testosterone, prostate and sexual healthand functions as an antibacterial agent in men’s urea system. It plays a role in epithelial integrity, showing that Zn is essential for maintaining the lining of the reproductive organs and may have a regulative role in the progress of capacitation and acrosome reaction. In contrast, Zn deficiency impedes spermatogenesis and is a reason for sperm abnormalities and has a negative effect on serum testosterone concentration. Based on these findings, Zn microelement is very essential for male fertility. It could be considered as a nutrient marker with many potentials in prevention, diagnosis, and treatment of male infertility.
    Keywords: Germination, Male fertility, Microelement, ROS, Sperm Parameters, Zinc}
  • Fereshte Bagheri, Reza Tahvilian *, Naser Karimi, Maryam Chalabi, Mahsa Azami
    The objective of this research was in-vitro germination and callus induction of Onosma bulbotrichum (O. bulbotrichum) as a medicinal herb which belongs to Boraginaceae family. For germination, the seedswere cultured on growth regulator-free MS medium and for callus induction, seeds were sown on modified MS medium containing different concentrations of kinetin (kn)- Indole-3-acetic acid (IAA) and kn- 2,4-D (2,4-dichlorophenoxyacetic acid), respectively. The plates were maintained in the dark at growth chamber. After 7 days seed germination on hormone-free medium and after 10 days callus initiation on modified medium in the presence of hormones was occurred. The maximum pigmented callus (100%) was observed on modified MS medium with a combination of 0.2 mg.L-1 IAA 2.10 mg.L-1 kn. Shikonin determination was performed by HPLC method. In addition, total hydroxynaphtoquinons as polyphenols in sum of callus and culture medium were measured by spectrophotometric method and revealed that total naphtoquinones content at IAA was more than 2, 4-D.
    Keywords: Onosma bulbotrichom, Germination, Callus, Indole, 3, acetic acid, 2, 4, Dichlorophenoxyacetic acid, HPLC}
  • مهین کاربر قریه حصار، حسین علیدادی، نورعلی نیکروح
    زمینه و هدف
    استفاده از ورمی کمپوست از جایگاه ویژه ای در کشاورزی برخوردار است. این امکان وجود دارد که گیاهان زراعی نسبت به کود تهیه شده در مراحل اولیه رشد حساس باشند، لذا مطالعه حاضر با بررسی تاثیر جوانه زنی و وضعیت فیزیولوژیکی گیاه ترتیزک با استفاده از ترکیب ورمی کمپوست های مختلف انجام شد.
    مواد و روش ها
    در این مطالعه به بررسی اثر جوانه زنی گیاه ترتیزک (شاهی) با استفاده از عصاره ورمی کمپوست با درصد حجمی 0، 15، 30 و 45 درصد حجمی از ورمی کمپوست تهیه شده از فضولات گاو، زباله میوه، کاغذ، خاک اره و برگ توسط کرم خاکی Eiseniafoetida پرداخته شد. تجزیه و تحلیل داده ها با استفاده از نرم افزار آماری SPSS (نسخه 11/5) و آزمون واریانس یک طرفه(One-way ANOVA) و دوطرفه(Two-way ANOVA) انجام گرفت. میزانp کمتر از 0/05 معنی دار در نظر گرفته شد.
    یافته ها
    بر اساس نتایج مطالعه حاضر، درصدهای حجمی متفاوت در رشد گیاه ترتیزک موثر بودند. میانگین رشد در درصد حجمی 30 نسبت به درصد حجمی 15 و 45 اختلاف معناداری داشت و کمتر از آن ها بود. سطح 15 درصد حجمی تهیه شده از کود ورمی کمپوست تولیدی، جهت رشد گیاه و جوانه زنی سطح مناسبی می باشد.
    نتیجه گیری
    کود ورمی کمپوست ترکیبی تهیه شده با درصد حجمی 15 درصد می تواند نقش موثری در افزایش کارایی تولید گیاه ترتیزک داشته باشد.
    کلید واژگان: جوانه زنی, عصاره ورمی کمپوست, گیاه ترتیزک, میزان رشد}
    Mahin Karbar, Hussein Alidade, Noorali Nikrooh
    Background &Objeftive: Vermicompost application plays an important role in agriculture. Crop plants can be sensitive to negative effects of vermicompost at early stages of development. The aim of the present study was to test the effects of different vermicomposts on seed germination of lepidiumsativum species.
    Materials and Methods
    In the current work, the effects of different vermicomposts’ extracts, which were produced from cow manure, fruit waste, paper, sawdust and leaves, on s germination and early development of lepidiumsativum were investigated in various volumetric percentages (0%, 15%, 30% and 45%). For data analysis, Statistical Package for Social Science (SPSS) program and ANOVA test were used.
    Results
    The results showed that different volumetric percentages were effective on lepidiumsativum growth. Average growth in volumetric percentage of 30% was less than 15 and 45%. In the way that presence differences was significant. Volumetric percentage of 15% was in appropriate level for plant growth and seed germination.
    Conclusion
    It can be concluded that the use of vermicompost extracts can have an effective role in production efficiency increase of lepidiumsativum.
    Keywords: Vermicomposting extract, germination, Growth rate, lepidiumsativum}
  • Abbas Haghparast, Kamran Mansouri, Samane Moradi, Fatemeh Dadashi, Saeed Eliasi, Mahdi Sobhani, Kambiz Varmira
    The present study investigated the radioprotective efficacy of lentil (Lens culinaris) sprouts against X-ray radiation-induced cellular damage. Lentil seeds were dark germinated at low temperature and the sprout extract was prepared in PBS. Free radical scavenging of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and then the radioprotective potency of extract (0 to 1000 µg/mL) on the lymphocyte cells was determined by lactate dehydrogenases assay. Moreover, micronuclei assay was assessed using the cytokinesis-block technique. The irradiations were performed using 6 MV X-ray beam. The value of IC50 for DPPH assay was 250 µg/mL. The median lethal dose for radiation was determinate at 5.37 Gy. Pretreatment with lentil sprout extract at 1000 µg/mL reduced cytotoxicity at 6 Gy total concentration from 70% to 50%. The results of micronuclei assay indicated that cells were resistant to radiation at concentrations of 500-1000 µg/mL of exogenous lentil sprout extract. The value of median effective concentration for micronuclei assay was 500 µg/mL. The results indicated that lentil sprout extract showed actually somewhat radioprotective effect on lymphocyte cell. In addition, the obtained results suggest that extract of total lentil sprout have more antioxidant activity than radicle part.
    Keywords: Radioprotective agents, Germination, X-Radiation, Legumes}
  • Zahra Alsadat Mirmohamadi, Monir Doudi *, Sima Yahyaabadi
    This study was conducted in acompletely randomized block design with three replications using three concentrations of 107, 105, and 103cfu/ml of Azotobacter isolated from the rhizosphere of Karun varieties of barley (Az. salentris) and standard Az.chrococom along with control (zero concentration of bacteria). The aim of this study was to study the reactions of barley plantin response to inoculation by mentioned bacteria in the form of infected separate inoculation and non-inoculated seeds as a control. For isolation the number of mentioned bacteria, Karun variety of barley in desert was cultivated in the mannitol broth environment, and macroscopic and microscopic characteristics of bacterial colonies were evaluated. Finally, using gene 16Sr DNA, isolated variety was identified molecularly. At the end of plant growth, important traits such as percent of germination, total protein level, and activity of antioxidant enzymes of catalase and peroxide were measured from the desired plant and they were analyzed statistically. After 18 days, results showed that the concentration of 107, 105, 103cfu / ml of Azotobacter isolated from the rhizosphereKarun variety of barley plant in the desert (Az. salentris) increased the activity of antioxidant enzymes, total protein, and percentage of germination compared with control. In addition, different concentrations of Azotobacter isolated from the rhizosphere of barley plant (Az. salentris) showed higher and significant impact on catalase activity, total protein level, and percentage of Karun variety of barley germination compared with standard Az.chrococom.
    Keywords: Antioxidant enzymes, Total protein, Azotobacter, Colony, PCR, Germination, Karun variety of Barley}
  • Siavash Moushekhian, Masoud Shiehzadeh, Amir Shammas
    The anatomic anomalies detection is important for an attentive clinician. A successful treatment of an endodontically involved tooth should make it functional and aesthetically acceptable for the patient. The following article presented a case of gemination and the endodontic, prosthetic and periodontal treatments which were done to keep a complicated tooth in aesthetic zone
    Keywords: Clinical management, germination, maxillary lateral incisor}
  • علی اکبر عامری، حجت الله ربانی نسب، محمدرضا جلیلوند، مهدی ایمانی
    زمینه و هدف
    مرحله جوانه زنی از اساسی ترین مراحل رشد گیاهان دارویی است که ممکن است توسط مواد شیمیایی که توسط سایر گیاهان در محیط خاک منتشر می شود تحت تاثیر منفی قرار گیرد. به منظور بررسی اثرات دگر آسیبی علفهای هرز تاج خروس (L. Amaranthus retroflexus)، سلمه (Chenopodium albumL.)، پنجه مرغی (L. Cynodon dactylon)، اویارسلام (L. Cyperus rotondus)، تاجریزی سیاه (L. Solanum nigrum) و تاتوره (L. Daturastramonium) که از علفهای هرز رایج مزارع همیشه بهار می باشند، مطالعه ای بر روی درصد و سرعت جوانه زنی و طول ریشه چه و ساقه چه گیاه همیشه بهار، در شرایط آزمایشگاه انجام گرفت.
    مواد و روش کار
    تیمارهای آزمایش شامل عصاره استخراج شده توسط آب مقطر از برگ های خشک شده شش گونه علفهای هرز بود که باچهار غلظت صفر (آب مقطر)، 5/2، 5 و 5/7 درصد حجمی (w/v) روی بذرهای گیاه همیشه بهار اعمال گردید. آزمایش در قالب فاکتوریل با طرح پایه کاملا تصادفی در چهار تکرار اجرا گردید.
    یافته ها
    نتایج آزمایش نشان داد که عصاره علفهای هرز یاد شده در غلظتهای مختلف روی خصوصیات جوانه زنی بذرگیاه دارویی همیشه بهار اثرات بازدارندگی معنی دار دارند. عصاره پنجه مرغی بیشترین تاثیر منفی را روی درصد جوانه زنی بذر همیشه بهار داشت و میزان آنرا به 69/24 درصد رساند که نسبت به شاهد 7/57 درصد کاهش نشان داد.تاثیر عصاره تاتوره، اویارسلام و تاجریزی روی درصد جوانه زنی کمتر از پنجه مرغی بود. کمترین تاثیر منفی روی درصد جوانه زنی را عصاره تاج خروس از خود نشان داد و درصد جوانه زنی را 72/44 درصد کاهش داد که از لحاظ آماری در سطح 5 % معنی دار بود. تاثیر غلظت عصاره روی خصوصیات جوانه زنی بذر همیشه بهار نیز در سطح 5 % معنی دار بود و با افزایش غلظت عصاره، تاثیر منفی آن روی درصد و سرعت جوانه زنی و طول ریشه چه و ساقه چه همیشه بهار افزایش یافت. عصاره 5/2 % کمترین تاثیر و عصاره 5/7% درصد بیشترین تاثیر بازدارندگی را در مقایسه با شاهد نشان داد.
    نتیجه گیری
    در شرایط آزمایشگاهی بذرهای گیاه همیشه بهار به شدت تحت تاثیر اثرات بازدارندگی از جوانه زنی و رشد ریشه چه و ساقه چه ناشی از عصاره علفهای هرز مورد استفاده در آزمایش قرار می گیرند، که این اثرات بستگی شدیدی به غلظت عصاره علف هرز مورد آزمایش دارد.
    کلید واژگان: دگرآسیبی, علفهای هرز, جوانه زنی, رشدگیاهچه, همیشه بهار}
    Background and Objectives
    Germination is one of the essential period of plants growth that can be affected harmfully by chemical agents released by other palnts.The allelopathic effects of Redroot pigweed (Amaranthus retroflexus L.), Lambsquarters (Chenopodium album L.), Bermuda grass (Cynodon dactylon L.), Nut grass (Cyperus rotondus L.), Black nightshade (Solanum nigrum L.) and Datura (Datura stramonium L.) were investigated on germination characteristics of Marigold (Calendula officinalis L.) seeds in a growth chamber experiment.
    Material And Methods
    Experimental treatments were extracted by distillation from dried leaves of six species of weeds that applied by four concentrations included: zero (distilled water), 2.5, 5.0 and 7.5 volume percent (w / v) was applied on the seeds of Marigold plants. Experimental design was factorial experiment in a completely randomized design with four replications.
    Results
    The aqueous extracts applied to seed, significantly inhibited seed germination, germination rate, radicle and hypocotyl length of marigold with increasing of extract concentration. Bermuda grass extract showed the most inhibition effect on seed germination of Marigold and decreased it by 57/7%. The inhibition effect of Datura, Nut grass and Black nightshade extracts was less than Bermuda grass. Datura, Bermuda grass and Nut grass extract showed the most negative effect on germination rate of marigold and decreased it 68%. All weeds extracts significantly decreased the length of marigold radicle and hypocotyl. But Lambs quarters and Nut grass extracts showed the most inhibition effects.
    Conclusion
    These results suggest that extracts from shoot of weeds had potentially allelopathic effect. However the activity differed depending on weed species and extract concentration.
    Keywords: Allelopathy, Weeds, Germination, Marigold(Calendula officinalis)}
  • Babak Ghiassi Tarzi, Maryam Gharachorloo, Marzieh Baharinia, Alireza Mortazavi
    Germination is one of the most effective processes to improve the quality of legumes. Vitamins and some other compounds that might be considered beneficial as antioxidants, often change dramatically during the course of germination. Antioxidants might be defined as compounds which are capable of preventing, delaying or retarding the development of rancidity or other flavor deterioration in foods or as protective factors against the oxidative damage in the human body. In this research, three different solvents were employed to extract the phenolic compounds present in chickpea seeds and sprouts. Total phenolic contents were measured by Folin Ciocalteau method and the antioxidant activity was determined by two different methods including the assay of hydroxyl radical scavenging activity and the oven test method.For the later, different concentrations of extracts (0.02, 0.04, 0.06, 0.08 and 0.1% w/w) were added to tallow and the stabilities of the treatments were determined. Peroxide value and induction period measurements were used as means to evaluate the antioxidant activities. The results indicated that germination process modifies the antioxidant activity. Although the amount of phenolic compounds was higher when acetone solvent was employed, methanolic extract indicated better hydroxyl radical scavenging activity. The evaluation of the antioxidant activity of the extracts activity was concentration-dependent by delaying the indicated oxidation and increased when higher concentrations of the extracts were applied. Therefore, chickpea sprout flour or extract might be used as a source of natural antioxidants in functional foods or in the formulation of the oil-based supplements or medicine in the form of capsule.
    Keywords: Antioxidant activity, Chickpea, Germination, Phenolic compounds}
  • فاطمه چراغی *، سهراب محمودی، مجید جامی الاحمدی، سهیل پارسا
    مقدمه و هدف
     جوانه‏ زنی و استقرار گیاهان دارویی به علت قوه نامیه کمی ‏که بذور این گیاهان دارند عموما با مشکل مواجه است. پرایمینگ بذر از جمله روش‏هایی است که منجر به افزایش قابلیت جوانه‏ زنی در طیف وسیعی از گیاهان می‏شود. هدف کلی تحقیق حاضر تعیین موثرترین ماده پرایمینگ، غلظت و مدت زمان پرایمینگ بر جوانه‏ زنی و رشد گیاهچه گلپر بود.
    روش تحقیق
    آزمایشی به صورت فاکتوریل بر پایه طرح بلوک‏های کامل تصادفی در آزمایشگاه تحقیقاتی دانشکده کشاورزی، دانشگاه بیرجند به اجرا درآمد. فاکتورهای آزمایش شامل نوع ماده پرایمینگ (KNO3,CaCl2  و پلی اتیلن گلایکول)، سطوح پتانسیل اسمزی (5/0-، 1- و 5/1- مگاپاسکال) و مدت زمان تیمار (12 و 24 ساعت) بودند.
    نتایج و بحث
    نتایج نشان داد که نوع ماده پرایمینگ بر تمامی ‏شاخص‏های اندازه گیری شده اثر معنی دار دارد. سطح پتانسیل اسمزی بر سرعت جوانه‏ زنی تاثیر معنی داری داشته است. اثر مدت زمان پرایمینگ بر صفات سرعت جوانه ‏زنی و میانگین مدت جوانه ‏زنی معنی دار بود. در بین تیمارها در صفات درصد، سرعت و میانگین مدت جوانه‏ زنی و شاخص بنیه گیاهچه، تیمار CaCl2 در 5/0-  مگاپاسکال به مدت 24 ساعت بهترین درصد جوانه ‏زنی، تیمار پلی اتیلن گلایکول 1- مگاپاسکال به مدت 24 ساعت بهترین سرعت جوانه‏ زنی، تیمار KNO3 در 1- مگاپاسکال به مدت 24 ساعت کمترین میانگین مدت جوانه ‏زنی و تیمار پلی اتیلن گلایکول به مدت 12 ساعت و 5/1- مگاپاسکال بهترین شاخص بنیه گیاهچه را نشان دادند. بنابراین می‏توان نتیجه گرفت با اعمال تیمارهای مناسب پرایمینگ می‏توان باعث بهبود در جوانه‏ زنی گیاه دارویی گلپر شد.
    توصیه کاربردی/ صنعتی
    نتایج آزمایش نشان داد پرایمینگ موجب افزایش خصوصیات جوانه زنی در گیاه گلپر می‏شود. با توجه به این که از کومارین موجود در ریشه این گیاه در صنایع مختلف استفاده می‏شود و از سوی دیگر بذر این گیاه جوانه زنی ضعیفی دارد؛ می‏توان با پرایمینگ آن جوانه زنی و استقرار این گیاه را بهبود بخشید و موجب افزایش در تولید آن شد.
    کلید واژگان: گلپر, اسمو پرایمینگ, جوانه‏ زنی, بنیه گیاهچه}
    Fatemeh Cheraghi *, Sohrab Mahmoodi, Majid Jami Alahmadi, Sohayl Parsa
    Background & Aim
    Because of seed germination ability of medicinal plants seed is low and, generally exposed to problem. Priming is one of the techniques that help seeds to be germinated faster and uniformly.
    Experimental
    A laboratory experiment was carried out on germination and growth improvement in Heracleum persicum Desf.  by seed osmo-primming in Seed Technology Laboratory of Faculty of Agriculture in Birjand University, Iran. Statistical design was factorial RCBD with three replications. Factors were priming media (Polyethylen glycol 6000 (PEG), KNO3 and CaCl2), osmotic potential of solution (-0.5, -1 and -1.5 MPa) and time of treatment (12 and 24 h).
    Results &Discussion
    The results showed that priming media had significant effect on all of evaluated characteristics. Germination rate significantly was affected by osmotic potential of solutions. Time of treatment was also effective on germination rate and MGT. However, CaCl2 in 24h and -0.5 MPa, PEG in 24h and -1 MPa, PEG in 12h and -1.5 MPa and KNO3 in 24h and -1 Mpa were best treatments in germination percentage and rate, seedling vigor index and MGT respectively. Therefore, suitable priming treatments are able to improve germination traits in Heracleum persicum.
    Industrial and Practical Recommendations
    The findings showed that priming leads to increasing the traits of blossoming in hogweed. Since cumarin is used in various industries and its seed is week in blossoming, using priming can be helpful for blossoming and fixating of this plant and can be increase the production.
    Keywords: Heracleum persicum, Desf.Osmopriming, Germination, Seedling vigorMGT}
  • ساره ظفریان *، سعدالله هوشمند، وحید روحی
    مقدمه و هدف
    کرفس کوهی یا کلوس (‏Kelussia odoratissima Mozaff.‎‏) یکی از گیاهان مرتعی و دارویی خانواده ی چتریان و بومی دامنه ی رشته ‏کوه های زاگرس است که متاسفانه به علت برداشت غیرمجاز در معرض انقراض می باشد. به منظور شکستن خواب و ارزیابی ‏اثر تیمارهای مختلف طی یک آزمایش فاکتوریل در قالب طرح کاملا تصادفی بر بذور منطقه ی سر آقاسید از توابع استان ‏چهارمحال و بختیاری سه دمای 4، 8 و 22 درجه ی سانتی گراد و اثر سال در دو سطح، مربوط به سال های متوالی 1388 و 1389 ‏در 4 تکرار در دانشکده کشاورزی دانشگاه شهرکرد انجام شد.
    روش تحقیق
    در این آزمایش ویژگی های نظیر میزان جوانه زنی، طول و قطر ریشه چه، محور زیر لپه و دم برگ و هم چنین طول و عرض ‏برگ لپه ای در 100 روز پس از کاشت مورد بررسی قرار گرفت. ‏میانگین صفات حاصل از تیمارهای مختلف به روش LSD و با استفاده از نرم افزار آماری SAS مورد تجزیه و تحلیل قرار گرفت.
    نتایج و بحث
    نتایج نشان داد که میزان جوانه زنی به طور بسیار معنی داری (0.01≥p) تحت تاثیر دما و سال و اثر متقابل این دو عامل قرار گرفت؛ به طوری که بالاترین میزان جوانه زنی مربوط به 4 درجه ی سانتی گراد بوده و در 22 درجه ی سانتی گراد جوانه زنی مشاهده نشد. هم چنین از لحاظ تاثیر سال، جوانه زنی در بذور مربوط به سال 1388 رخ داد و بذور مربوط به سال 1390 نیز جوانه زنی صورت نگرفت. مقایسه میانگین صفات در درجه حرارت 4 و 8 درجه سانتی گراد نشان داد به جز جوانه زنی سایر صفات دمای 8 درجه ی سانتی گراد میزان بالاتری را نشان دادند، هر چند تنها دو صفت طول و عرض برگ به طور معنی داری (0.01≥p) در درجه حرارت 8 درجه سانتی گراد بیشتر بود.
    توصیه کاربردی/ صنعتی
    با توجه به این که مهم ترین دلیل کاهش پراکنش این گیاه طول دوره خواب بذر می باشد، لذا استفاده از نتایج این تحقیق به کوتاه کردن جوانه زنی و در نتیجه اهلی کردن این گیاه دارویی کمک خواهد نمود.
    کلید واژگان: جوانه زنی, خواب بذر, دما, عمر بذر, کرفس کوهی}
    Sare Zafarian *, Saadollah Houshmand, Vahid Rouhi
    Background & Aim
    Kelussia odoratissima Mozaff. (Apiaceae) is one of the medicinal plants and endemic in Zagros Mountains, Iran, which unfortunately due to illegal harvest is endangered. In order to break dormancy and to evaluation of effect of different treatments on traits a factorial experiment base on completely randomized design was created on the seed zone Sar-aghaseyed functions in Chaharmahal va Bakhtiari province, in four replications in  Faculty of Agriculture, Shahrekord University, Iran. The treatments were included three temperatures 4, 8 and 22 °C and years in the successive years 2009 and 2010.
    Experimental:
    In this experiment, germination characteristics, rootlet length and diameter, hypocotyls, and petiole, and also leaf length and width were recorded 100 days after planting. The results indicated germination was affected significantly (pResults &
    Discussion
    The traits  means comparison of 4 °C  and 8 °C degreases  indicated all the traits were expressed better in 8 °C degrees with the exception of germination, However leaf length and width were significantly (p Industrial and Practical Recommendations: Since the most important reason in decreasing the percentage of this plant is the rate  of sleep duration of seed, using the findings of this research can help to decrease the duration of germination and, as a result,  to domesticate this kind of medicinal plant.
    Keywords: Germination, Kelussia odoratissima Mozaff, Seed aged, Seed dormancy, Temperature}
نکته
  • نتایج بر اساس تاریخ انتشار مرتب شده‌اند.
  • کلیدواژه مورد نظر شما تنها در فیلد کلیدواژگان مقالات جستجو شده‌است. به منظور حذف نتایج غیر مرتبط، جستجو تنها در مقالات مجلاتی انجام شده که با مجله ماخذ هم موضوع هستند.
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درخواست پشتیبانی - گزارش اشکال