جستجوی مقالات مرتبط با کلیدواژه « glutathione peroxidase 1 » در نشریات گروه « پزشکی »

There is ample evidence regarding the protective effects of antioxidant vitamins and minerals against oxidative stress. The discovery of zinc's protective role in countering free-radical formation and oxidative stress has instigated extensive research into the antioxidant properties of zinc and its involvement in the antioxidant defense system. Furthermore, the utilization of plants with antioxidant properties has gained increasing attention among researchers. This study aims to investigate the impact of six weeks of aerobic exercise training, along with supplementation of pumpkin seed oil and chickpeas, on the gene expression of antioxidant enzymes in the liver and muscle tissues of male rats.

To achieve this objective, 36 Wistar rats were divided into six groups: control (C), training (T), chickpea (Ch), chickpea + training (Ch+T), pumpkin seed oil (P), and pumpkin seed oil + training (P+T). At the conclusion of the training and supplementation period, the gene expression of the antioxidant enzymes glutathione peroxidase 1 (GPX1) and catalase (CAT) were assessed. Data were subjected to one-way analysis of variance at a significance level of P < 0.05.

The results revealed that in all the groups involving pumpkin seed oil and chickpea supplementation, in both liver and muscle tissues, a non-significant decrease in the expression of the enzymes CAT and GPX1 was observed when compared to the control and exercise training groups.

To a certain extent, the use of pumpkin seed oil and chickpeas may enhance the expression of antioxidant enzymes.

Asthma, a prevalent chronic airway inflammatory condition, poses a significant health challenge. In this study, we delved into the regulatory mechanisms governing asthma, focusing on Methyltransferase-like 3 (METTL3). Through an ovalbumin (OVA)-induced mouse model and interleukin-13 (IL-13)-induced cell model, we mimicked the in vivo and in vitro functions of METTL3 in asthma. Our research revealed that METTL3 expression significantly decreased in asthma-induced mice and IL-13-stimulated cells compared to the control group. Moreover, METTL3 overexpression enhanced bronchial epithelial cell viability and proliferation. Mechanistically, we observed elevated levels of total iron, Fe2+, malondialdehyde (MDA), lipid reactive oxygen species (ROS), alongside reduced glutathione (GSH) levels in IL-13-stimulated cells. Remarkably, METTL3 overexpression counteracted these effects, suggesting a pivotal role in mitigating asthma-related oxidative stress. Furthermore, our study highlighted the involvement of N6-methyladenosine methylation (m6A) modification, where METTL3 regulated the m6A modification of glutathione peroxidase 4 (GPX4) RNA, impacting RNA stability. Knockdown of METTL3 suppressed m6A modification on GPX4 RNA, impairing its stability and contributing to IL-13-induced ferroptosis. Interestingly, METTL3 overexpression not only inhibited cell ferroptosis but also alleviated asthma symptoms. Our findings shed light on the epigenetic regulation of asthma through METTL3-mediated m6A modification, offering potential therapeutic avenues for this prevalent inflammatory disease.

Tannery effluents add pollutants to the aquatic environment. The characterization of responses to toxic exposure at the molecular level of biological systems is a major challenge in ecotoxicology because it enables the unraveling of mechanisms of toxicity, the discovery of novel biochemical markers, and early diagnoses of exposure and effects. The three major classes of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) were analyzed in the liver, testes, and ovary of Channa striatus to delineate the impact of tannery effluents on these enzymes. The oxidative stress biomarkers showed a significant reduction (p<0.05) in their activities among the control and experimental groups exposed to both 10 and 1 % concentrations of the tannery effluents. The SOD activity was reduced to 96.13 % in the ovary compared to the liver (60.73 %) and testes (47.89 %) after 30 days of exposure to a 10 % concentration of the tannery effluents. However, the activities of catalase and glutathione peroxidase decreased to 58.59 % and 43.64 % in the testes when compared to the liver and ovary.

This study aimed to investigate the GPx-1 gene polymorphism (rs1050450), the level of oxidative stress and antioxidant parameters, and the lipid profile in an obese Kurdish population in Sulaimani, Iraq.

In a case-control study,134 obese subjects and 131 normal BMI healthy individuals participated. The GPx-1 gene polymorphism was assessed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. The levels of biochemical and oxidative parameters were determined using photometric methods.

The results showed that the fasting blood sugar (FBS), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C) levels were significantly higher in obese subjects compared to the control group. Obese individuals had significantly lower levels of high-density lipoprotein cholesterol (HDL-C) than the controls. The GPx-1 activity and total antioxidant capacity (TAC) levels were significantly elevated in the obese group compared to the control group (P=0.006, and P<0.001, respectively). No significant difference was detected in genotype and allele frequencies of GPx-1 (rs1050450) between obese and normal BMI groups. However, the presence of the GPx-1 TT genotype enhanced the risk of obesity in females by 1.93-fold (95% CI 1.04-3.58, P=0.036). In the total population, the GPx activity increased in the presence of TT compared to CC+CT and CT genotypes.

The study indicated that obesity is linked to significantly higher levels of FBS, TG, LDL-C, TAC, and GPx activity and lower level of HDL-C. Also, we found the GPx-1 gene polymorphism was associated with the risk of obesity in females and increased the GPx activity.

Introduction. Nephrolithiasis is a common multifactorial kidney disease with worldwide distribution. Compelling evidence, regarding the function of kidney in maintaining the body homeostasis, suggests the role of oxidative stress in the pathogenesis of nephrolithiasis. Glutathione peroxidase 1 is a major antioxidant enzyme, preventing oxidative damage to renal cells by detoxifying hydrogen and lipid peroxides, which may involve in its pathogenesis. The purpose of the present study was to determine the possible association of glutathione peroxidase 1 gene (GPX1) proline-to-leucine substitution at amino acid 198 (Pro198Leu polymorphism) with the risk of developing nephrolithiasis in south Iranian patients.
Materials and Methods. Association of Pro198Leu polymorphism in exon 2 of GPX1 gene was investigated in 150 patients with nephrolithiasis and 184 healthy age-, sex-, and ethnically-matched control group using polymerase chain reaction-restriction fragment length polymorphism.
Results. Regression analysis demonstrated that the frequency of the genotypes carrying at least 1 Leu allele, in both dominant and codominant model for this allele, was significantly higher in patients compared with the controls. However, significant association was found neither with wild-type allele, nor with polymorphic allele with the risk of nephrolithiasis.
Conclusions. Findings of our study provide potential support in favor of the role of oxidative stress in the pathogenesis of nephrolithiasis in patients from south of Iran. The results indicate that GPX1 may be a key player in nephrolithiasis development.

Oxidative stress plays a major pathogenic role in liver injury following chronic hepatitis B. Glutathione peroxidase (Gpx) has a central role in regulating the oxidative state. Hepatitis B virus (HBV) results in down-regulation of Gpx. On the other hand, iron homeostasis is disrupted in HBV infected patients. Therefore, the objective of this study was to assess the interplay of Gpx and serum iron on clinical and virological characteristics of patients with chronic HBV infection.
One hundred and fifty adult, treatment-naïve, patients with chronic hepatitis B were randomly selected from an ongoing cohort of patients with HBV. Plasma Gpx1 concentration and HBV DNA quantity were measured. Liver stiffness was measured by transient elastography.
Serum iron had a positive association with HBV DNA count in the total population. Serum iron was not associated with liver stiffness. However, HBV DNA was significantly associated with liver stiffness only in male patients. Serum Gpx was inversely associated with liver stiffness. Serum iron and Gpx had indirect effects on liver stiffness via HBV DNA count. We observed distinct effects of serum iron on HBV DNA and Gpx on liver stiffness in male and female patients.
We identified interplay of serum iron and Gpx1 in relation to level of liver fibrosis in patients with chronic hepatitis B. Our results suggest that oxidative stress and serum iron are differentially implicated in the progression of chronic hepatitis B in male and female patients.

Recurrent aphthous stomatitis (RAS) is a common disease with unknown etiology and no treatment. The purpose of this study is to assess the relationship between serum antioxidants level and clinical manifestation. Change in antioxidant level can lead inflammatory reaction through oxidative stress.
A total 50 patients were selected for this cross sectional study. Superoxide dismutase (SOD), glutathione peroxidase (GSHPX) and total antioxidant status (TAS) was measured in plasma. Also pain intensity based on the visual analogue scale (VAS), the mean interval between lesion, number of lesions and the mean duration of complete healing were recorded. Statistical analysis was performed by using spearman, chi square and T-test.
We had 50 patients include 28 female and 22 male. Mean and standard deviation was for TAS (1.35±0.15), GPX (178.42±39.67) and SOD (257.86±63.17). Number of lesion and duration of RAS had direct relationship with pain severity. Pain intensity and GPX and SOD were significant (respectively 0.05, 0.04). GPX and SOD level was indirectly related (p=0.04).
We could show that RAS patients had alteration in SOD and GPX level but TAS level was stable. Against TAS level which had no change, with more lesion and severe pain GPX level is lower and SOD level was higher. These two antioxidants were related indirectly.