جستجوی مقالات مرتبط با کلیدواژه "hexane extract" در نشریات گروه "پزشکی"
جستجوی hexane extract در مقالات مجلات علمی
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Background & Aim
Medical conditions associated with pains and inflammation are prevalent in the global population. The tubers of Chlorophytum alismifolium are widely used traditionally in Nigeria for the management of pain and inflammation. This study aims to establish the chemical profile and evaluate the anti-inflammatory and analgesic activities of the hexane extract of C. alismifolium (HECA) tubers in experimental animals.
ExperimentalGas chromatography-mass spectrometry (GC-MS), phytochemical screening and acute toxicity studies were conducted according to standard protocols. Analgesic activity was investigated with graded doses of HECA (250, 500 and 1000 mg/kg) using acetic acid-induced writhing test in mice and formalin-induced pain in rats; while the anti-inflammatory activity was evaluated using carrageenan-induced paw oedema model in rats.
ResultsThe GC-MS analysis revealed the presence of eighteen compounds covering an area of approximately 100%. Phytochemical screening revealed the presence of flavonoids, alkaloids, steroids and triterpenes while the oral median lethal dose was estimated to be >5000 mg/kg in rats and mice. In the 4th and 5th hour of the carrageenan test, HECA at 500 and 1000 mg/kg significantly (P<0.01) reduced the oedema index, respectively. In the analgesic study, HECA significantly (P<0.001) reduced the mean number of writhes with the highest inhibition (79.67%) obtained at 500 mg/kg. In the formalin test, HECA at 250 mg/kg significantly (P<0.05) reduced the mean pain scores in both phases of the test.
Recommended applications/industriesThe findings depict that HECA possesses pharmacologically active compounds that can be applied in the management of inflammation and pain.
Keywords: Chlorophytum alismifolium, GC-MS, Hexane Extract, Inflammation -
مقدمهتیروزیناز یک پلی فنل اکسیداز حاوی مس است که در ملانوژنز دخالت داشته و در فرایند قهوه ای شدن آنزیمی، که موجب تغییر رنگ و از دست دادن ارزش غذایی در محصولات گیاهی می شود. تیروزیناز نقش با اهمیتی را به عنوان کاتالیزور آنزیمی بازی می کند.هدفتعیین درصد مهار، تحلیل سنتیکی مهار تیروزیناز و تعیین اثر آنتیاکسیدانی عصاره هگزانی اندام های هوایی گیاهان گون و کنگر.روش بررسیعصاره هگزانی گیاهان به روش خیساندن در هگزان و تغلیظ حرارتی به دست آمد. اثر مهاری عصاره ها در چهار غلظت 1، 1/0، 01/0 و 001/0 میلی گرم در میلی لیتر در طول موج 492 نانومتر با استفاده از دستگاه میکروپلیت ریدر تعیین شد. از اسید کوجیک به عنوان کنترل مثبت استفاده شد. تعیین فعالیت آنتیاکسیدانی با استفاده از DPPH انجام و از آسکوربیک اسید به عنوان کنترل مثبت استفاده شد. در تعیین ترکیبات عصاره ها ازدستگاه GC/MS استفاده شد.نتایجفعالیت مهاری عصاره هگزانی برگ گیاه گون برابر 6/66 درصد و برای برگ و گل گیاه کنگر به ترتیب برابر با 7/71 و 7/68 درصد بود. مقدار IC50 برای برگ گیاه گون 5/0 میلی گرم در میلی لیتر و برای برگ و گل گیاه کنگر به ترتیب برابر 5/0 و 32/0 میلی گرم در میلی لیتر بود. برگ گیاه گون نیز میزان فعالیت آنتیاکسیدانی قابل توجهی از خود نشان داد. آنالیز GC/MS نتایج معنیداری در زمینه معرفی ترکیبات بالقوه مهارکننده به دست نداد.
عصاره های هگزانی تهیه شده از اندام های گیاهانی که بیشترین اثر مهارکنندگی تیروزیناز را از خود نشان دادند جهت تهیه مهار کننده های جدید این آنزیم با هدف تولید داروهایی با اثرات جانبی کمتر مناسب هستند.
نتیجه گیریکلید واژگان: Astragalus vegetus Bunge, تیروزیناز, عصاره هگزانی, Gundelia tournifortii LBackgroundThe enzyme tyrosinase is a copper-containing polyphenol oxidase, which is involved in melanogenesis. It catalyzes the conversion of tyrosine to melanin pigments in mammals. Tyrosinase plays an important role in enzymatic browning, which causes discoloration and loss economic value of many plant food products. This enzyme is widely distributed in fungi, higher plants and animals.ObjectiveThe aim of this study was to determine the tyrosinase inhibitory effect of hexane extracts of different aerial parts of Astragalus vegetus Bungi and Gundelia turnifortii L. Later the kinetics and antioxidant activities of the extracts with the highest inhibitory properties were assessed.MethodsAerial parts of the plants were divided to stems, leaves and flowers and air-dried. Hexane extract of each part has obtained by maceration in n-hexane. Inhibitory effects assayed at four different concentrations, using a microplate reader at 492 nm. Kojic acid used as positive control. DPPH method used to determine the antioxidant activities of the extracts and ascorbic acid (positive control). GC/MS device utilized to determine the composition of the extracts.ResultsMain inhibitory activity detected at 1 mg/ml concentration of hexane extract of leaves of Astragalus vegetus Bunge (66.6%), and the leaves and flowers of Gundelia turnifortii L. (71.7 and 68.7 percent respectively). IC50 values for Astragalus vegetus Bungi leaves was 0.5 mg/ml. and for Gundelia turnifortii L. leaves and flowers, were 0.5 and 0.32 mg/ml respectively. Astragalus vegetus Bungi.plant leaves also showed a considerable antioxidant activity. GC/MS analysis of extracts didn't show any reliable result.ConclusionThese results suggest that some of the most effective extracts from analyzed plants in this study, may be worthy for further investigation to obtain some new tyrosinase inhibitors with pharmacological applications.Keywords: Astragalus vegetus Bungi, Gundelia turnifortii L., Hexane extract, Tyrosinase -
International Journal of Molecular and Clinical Microbiology, Volume:8 Issue: 2, Summer and Autumn 2018, PP 993 -1000Herpes simplex virus type 1 (HSV-1) leads to various clinical manifestations in humans. Due to drug resistance, the use of herbs extracts for treatment has been considered as a significant solution. One of these compounds is Chelidonium majus L. herb extracts which is known as Mamiran in Iran. In this study, two aqueous and hexane extracts of Chelidonium majus L. were used to investigate the antiviral effects against HSV-1. The toxicity of hexane and aqueous extracts on HeLa cells was determined by MTT assay. Anti-viral effects of the extracts were evaluated directly, at different concentrations and times. The effect of the aforementioned extracts on reducing the virus replication was evaluated by quantitative Real-time PCR based on the glycoprotein D (gD) gene expression level of the herpes simplex virus. We observed that a 3500 μg/ml concentration of aqueous extract of C.majus and 600 μg/ml concentration of hexane extract were not toxic to HeLa cells. None of the extracts had a direct anti-viral effect on the virus. The aqueous extract at the above concentrations after 1, 2, 4, and 8 h of adsorption significantly reduced on virus replication. In contrast, the hexanic extract of this plant had no viral and inhibitory effect on HSV-1 replication. The aqueous extract of C.majus has an antiviral effect on HSV-1 compared to the hexane extract.Keywords: HSV-1, Chelidonium majus, Hexane Extract, Aqueous extract, Real-time PCR
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In this study, both the presence and concentration of some unsaturated compounds in hexane extracts of different parts of Anthemis talyschensis showing absorption at wavelength 280-450 nm were surveyed, with the view of possibly using extracts of this plant in new formulations of sunscreen creams. The hexane extracts of flower, leaf and stem of A. talyschensis, collected from Northwest Iran, were obtained using a Soxhlet apparatus. The fatty acids were derivatized to methyl esters and were determined by gas chromatography/flame ionization detector (GC/FID) and gas chromatography/mass spectrometry (GC/MS) systems. The chemical analysis resulted in identification of 14, 9 and 29 constituents, comprising about 99.5, 97.1 and 98.2% of the total constituents in hexane extracts of flower, leaf and stem, respectively. The main unsaturated constituents in the hexane extract of A. talyschensis flower were 9, 12-octadecadienoic acid, 9-octadecenoic acid and 6, 9, 12-octadecatrienoic acid; while the leaf's extract contained 9, 12-octadecadienoic acid and 9-octadecenoic acid; no unsaturated compounds were detected in the stem. The ratios of unsaturated fatty acid /saturated fatty acid were 13.6, 9.3 and 0 in extracts of the flower, leaf and stem, respectively, but the total amounts in the leaf were much greater. It can be concluded the leaf extract is more likely to be suitable for producing sunscreens creams than others.Keywords: Anthemis talyschensis, Hexane extract, Unsaturated fatty acid, UV light, Sunscreen
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Vitexagnus- castus L. [Vac.] has been used in the Iranian traditional medicine for the treatment of pain and swelling of uterus. In this study, GC and GC/MS analyses were carried out for the identification of essential oil chemical components. Formalin and Xylene-induced ear-edema were used in order to nociception and inflammation activity. Then, the possible interaction between 3drugs including Naloxone (2mg/kg), Dextrometorphane(20mg/kg), and L-NAME (10mg/kg) have been used and Vitexagnus-castus hexane extract was examined. 1,8-Cineole (23 %), alpha-Pinene (16 %), beta- Pinene (13 %),, Z- Caryophylene (11 %), alpha- Terpinyl acetate (9 %), E- caryophylene (9 %) and Linalool (6.5 %) were the major identified components of the essential oil of Vitexagnus- castus L. Hexane extract was reduced licking time as compared to the control group in the first and second phase of formalin test. In Xylene-induced ear-edema, the hexane extract of Vitexagnus- castus fruits was strongly inhibited inflammation as compared to the positive control group. Interaction between L-NAME and Vitexagnus-castus hexane extract showed significant effect. It was concluded that the anti-inflammatory and antinociceptive effects of the fruit of Vitexagnus- castus L. may be due to its very pharmacological effective essential oil components. Interaction between L-NAME and Vitexagnus-castus hexane extract showed that one of the possible pathways is NO pathway, but Vitexagnus-castus hexane extract probably acts via other pathways that need more research.Keywords: Vitexagnus, castus L., Hexane extract, GC, MS, anti, inflammation, antinociception, Male mice
نکته
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