جستجوی مقالات مرتبط با کلیدواژه "hla drb1" در نشریات گروه "پزشکی"

  Hepatitis C virus (HCV) is a major cause of chronic liver infection, which may lead to liver cirrhosis, fibrosis, and hepatocellular carcinoma while about one-fourth of the infected patients recover spontaneously. The host immune response remains decisive in the outcomes of antiviral treatment. Single nucleotide polymorphisms (SNPs) at the genes within the human leukocyte antigen (HLA) cluster are associated with differential immune response and treatment outcomes. This study aimed to determine the association of SNPs in Tumor necrosis factor-alpha (TNF-) and HLA genes (HLA-DRB1and HLA-DQB1) with the outcomes of HCV infection and anti-HCV therapy. The study included 245 HCV-infected patients visiting a tertiary care hospital, located in Islamabad, Pakistan. Viral quantificationandgenotyping were performed by real-timePCR. Twenty SNPs in TNF-, HLA-DRB1, andHLA-DQB1 were sequence-genotyped by the Sanger method in 180 patients. Multivariate logistic regression was performed to establish the association of SNPs with spontaneous clearance of the virus and response to anti-HCV therapy. Five out of 20 SNPs were novel. Allelic variants at three different locations (HLA-DRB1, rs2308802; HLA-DQB1,-8447, and HLADQB1,- 8471) showed significant associations with two groups of HCV patients receiving anti-HCV treatment (responsive and nonresponsive). Multivariate analyses disclosed that genotype A/A at HLA-DQB1 (-8471) was a significant predictor of positive response to treatment although in the presence of the variant at HLA-DRB1 (rs230880) (P = 0.004). However, no association was detected between the haplotypes constructed for the three sets of SNPs and different categories of patients. This study established a novel SNP HLA-DQB1(-8471) as an important predictor of an effective response to anti-HCV therapy in HCV-infected Pakistani patients. Prescreening of this variant before therapy would benefit HCV patients.

Human leukocyte antigen (HLA) complex is a gene family involved in antigen presentation associated with protection or susceptibility to inflammatory, infectious and autoimmune diseases. Atherosclerosis is a chronic inflammatory disease in which HLA molecules play a role in the initiation and development of the disease through presentation of self or foreign antigens to T cells. To investigate the association of HLA-DRB1 alleles with atherosclerosis in a sample of southwestern Iranians. We performed an analytical cross-sectional study involving 96 patients with atherosclerosis and 72 controls. HLA-DRB1 genotyping was performed by PCR-SSP method. We observed a significantly lower frequency of DRB1*01 in patients with coronary artery atherosclerosis than in controls (4.68% vs. 13.1, P=0.0052, OR=3.09, CI 95%: 1.35-7.05). However, this allele showed a positive association with high blood pressure (P=0.009) in patients. Furthermore, DRB1*16 allele was associated with hyperlipidemia (P=0.008) in patients. Our results demonstrated that DRB1*01 may be a protective allele against atherosclerosis in individuals who live in southwest of Iran. The mechanism of this protection needs further investigation

Sarcoidosis a systemic granulomatous disorder of unknown etiology with varying clinical pictures. HLA genes, especially HLA-DRB1, have been shown to be candidates for the etiology of sarcoidosis. This study examined the association between the polymorphism of HLA-DRB1 alleles and sarcoidosis in Iranian subjects. The study population included 58 patients with sarcoidosis and 68 healthy controls. Genomic DNA was extracted, and the polymorphisms of the HLA-DRB1 alleles were determined using a polymerase chain reaction with sequence-specific primer (PCR-SSP). The frequency of HLA-DRB1*07 was higher in sarcoidosis patients (25.8%) than in controls (15.3%); however no significant difference was observed between the two groups (P>0.05). The frequency of HLA-DRB1*11 was higher in the control group (31.9%) than in cases (22.4%), but no significant difference was detected between the groups (P>0.05). The results of the present study showed that there is no association between HLA-DRB1 alleles and susceptibility to sarcoidosis in Mashhad, Northeast Iran. Further studies with large sample sizes are required in order to clarify this issue.

People of all ages can su?er from Henoch?Schönlein purpura (HSP), but it is the most common vasculitis in childhood. Te most important involving gene is located on chromosome 6p21.3, a region coding for human leukocyte antigens (HLAs). Among HLA genes, because of the high rate of polymorphisms, HLA?DRB1 is estimated to have a strong association with HSP. In this study, we aimed to assess the association of HLA?DRB1 alleles with HSP in Iranian children.
Tis study consisted of thirty Iranian children with HSP and 35 healthy controls. Genomic DNA was extracted, and HLA typing was performed by polymerase chain reaction with sequence?specifc primers technique.
Te results have shown that HLA?DRB1*01 and HLA?DRB1*11 (P = 0.002, odds ratio [OR] = 7.579, confdence interval [CI] = 1.934–29.697 and P = 0.039, OR = 3.333, CI = 1.030–10.788), respectively, are the most frequent alleles associated with HSP in Iranian children population. Te frequency of other alleles was not signifcantly di?erent in both groups. Te results also show no correlation between HLA types and disease manifestations.
According to these results, there is an association between HLA?DRB1*01 and HLA?DRB1*11 gene polymorphisms and susceptibility to HSP in our study group.

Juvenile idiopathic arthritis (JIA) is one of the most common chronic rheumatic diseases in children. The complex nature of this immune-mediated disease owes itself to several predisposing genes and environmental factors affecting its pathogenesis. Conducted in Iran, this study was originally intended to investigate every possible association between HLA DRB1 alleles and a susceptibility to JIA.
In this case-control study, 45 patients with a definite diagnosis of JIA based on International League against Rheumatism (ILAR) criteria were compared against 46 healthy controls. DNA samples taken from both groups were analyzed using PCR-sequence specific primers (PCR-SSP) method. Data analysis including parametric and nonparametric test and multivariate analysis was undertaken using the SPSS 11.5 software. A P-value Mean ages in case group and healthy controls were 14.64±6.21 and 13.73±6.39, respectively with no significant difference between the two groups (P=0.515). Sex difference between JIA group and healthy controls was also not significant (P=0.068) .The frequency of HLA-DRB1*01 was found the most frequent HLA-RB1 in our patients (33.3%). No significant statistical correlation between various HLA-DRB1 alleles and clinical subtypes of the disease could be established from the data. HLA-DRB1*11 was shown to raise protection to JIA (P=0.035, OR=2.755, 95% CI=0.963-8.055) in northeastern Iran. In addition, we found that HLA-RB1*09 is nominally associated with an increased risk of JIA (P=0.56, OR=2, 05, 95% CI=0.18-23.63).
HLA-DRB1*11 was shown to raise protection to JIA in northeastern Iran. The disparity of findings in other ethnicities prompts further investigations with larger sample sizes.

The human leukocyte antigen-DRB1 (HLA-DRB1) locus is one of the most polymorphic human loci and has a crucial role in the immune system. Assessing the allelic frequencies of HLA-DRB1 locus would be a fundamental factor in defining the origin of populations, relationships with other populations, disease association studies and the constitution of unrelated bone marrow donor registries. In the current study HLA-DRB1 alleles and their frequencies are determined in a family-based study by DNA sequencing-based typing high-resolution (2 field) level of typing.
Genomic DNA from 3 members of 68 unrelated families (a total of 204 individuals) was extracted. Exon 2 of DRB1 gene was amplified and sequenced and allele assignment was performed using Assign™ SBT v4.7sequence analysis software.
We had DRB1*11:04 with frequency of 0.0931, DRB1*03:01 with 0.0882, DRB1*11:01 with 0.0735, DRB1*13:01 with 0.071 and also alleles DRB1*08:03, DRB1*13:42, DRB1*14:04 and DRB1*14:07 with frequency of 0.0024.
A total of 34 different alleles were found in the study subjects with DRB1*11:04, DRB1*03:01, DRB1*11:01 being the most frequent alleles respectively.