جستجوی مقالات مرتبط با کلیدواژه « ht-29 » در نشریات گروه « پزشکی »

Cancer is a neoplastic disease that continues to be a global challenge, with a reported prevalence increasing annually. Epidermal growth factor receptor (EGFR) is overexpressed in various epithelial tumors such as breast and colon cancer, and for this reason, it is used for cancer diagnosis and treatment. Because of the high mortality rate associated with cancer and the side effects of chemotherapy and radiotherapy, patients need replaced strategies for therapy. Ginger has biological effects, including antioxidant and anticancer activities.

This study aims to evaluate ginger extract’s effect on the expression of EGFR in MDA-mb231 and HT-29 cell lines.

Fresh ginger rhizomes were purchased from a local food market, washed, grated, and then ginger extract was prepared using ethanol. MDA-mb231 and HT-29 cell lines were treated with different concentrations of ginger (5, 10, and 20 gr/mL) extract in RPMI-1640 plus 10 % FCS for 18 h. The gene expression of EGFR was measured by real-time PCR.

The level of EGFR expression in MDA-mb231 and HT-29 cell lines after treatment with different concentrations of ginger extract was not changed significantly (P > 0.05).

The current data indicate that the ginger extract does not have a significant dose-dependent effect, in the concentration range of 5 to 20gr/mL, on the expression of EGFR in these cancer cell lines. It is suggested to repeat the experiments with higher concentrations of ginger and in a time-dependent manner.

Curcumin, a bioactive component of Curcuma langa, has been investigated for its anti-proliferative effects against various cancer cell lines. Although results are very promising, the poor water solubility and low bioavailability of curcumin are its main limitations for clinical application.

The purpose of this study was to develop a drug delivery system, consisting of hydroxyapatite (HAp) polymer and sodium alginate (NaAlg), covering the magnetic core of iron oxide nanoparticles (IONPs), and loaded with curcumin in order to enhance its bioavailability and therapeutic efficacy.

In this study, IONPs were prepared by the co-precipitation method and coated with HAp and NaAlg. The nanoparticles (NPs) were characterized by X-ray diffraction, Fourier Transform Infrared Spectroscopy (FTIR), and electron microscopy (TEM and SEM). Encapsulation efficiency and curcumin loading rate were examined. Drug release rate was also measured in vitro at pH = 7.5 and 5.5. The toxicity of curcumin-loaded NPs and free curcumin was evaluated against HT-29 and MCF-7 cancer cells.

The assessment of physicochemical characteristics showed the synthesis of spherical particles with nanometer sizes (5 - 7 nm) and a high encapsulation efficiency (84.16 ± 3.51 %) and drug loading capacity (21.03 ± 0.87%). Maximum drug release was obtained at pH = 5.5. Iron oxide nanoparticles showed no significant cytotoxic effects. Curcumin-loaded coated IONPs showed a higher toxicity against HT-29 and MCF-7 cancer cells compared to free curcumin.

This in vitro study showed that the encapsulation of curcumin, as a potent herbal drug, into IONPs enhanced its bioavailability, suggesting the NPs as an efficient vehicle for targeted drug delivery in cancer treatment.

Natural products are one of the major sources for investigations of novel medicines. Zataria multiflora Boiss (ZM) has shown pharmacological activities especially in gastrointestinal tract; however, there are limited studies about its cytotoxicity effects. In this study, the effect of Zataria multiflora was examined on two colon cancer cell lines (SW-48 and HT-29).

Hydro-alcoholic extract of ZM and its fractions including chloroform, petroleum ether and methanol extract were prepared by warm maceration method. Different concentrations were prepared and examined on SW-48 and HT-29 cell lines using 2-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay.

The results of the present study have shown the cytotoxic effect of some fractions of ZM. The most considerable cytotoxic effect was shown against HT-29 cell line. Also, total ZM extract and the petroleum ether fraction demonstrated cytotoxic effects with IC50 values of 44.22 and 33.42 µg/ml on SW-48 and HT-29 cell lines, respectively.

Zataria multiflora was cytotoxic to against colon cancer cell lines HT-29 and SW-48.

Colon cancer is the third most prevalent cancer in Iran. Prolonged colon inflammation is an important factor, in the development of colon cancer. Ginger has anti-inflammatory properties due to its content of [6]-gingerol and hence can play a role in the prevention of colon cancer. In this research the effects of ginger extract on reducing expression of the Cox-2 gene in HT-29 cells was studied in colon cancer.

In this laboratory research, HT-29 cells of colon cancer were and the cells were trypsinated at the third passage and 5000 of them were cultured in the wells of 96-well plates. Ginger extract at four concentrations (10, 20, 30, and 40 mg/mL) was added to the wells, and the plates were incubated at 37˚C for 24, 48, and 72 hours in a 5% Co2 atmosphere. To study the effects of ginger extract on the cells, the MTT test was performed and the densities of the plates were read using an ELISA instrument. Total cellular RNA was extracted and expression of the COX-2 gene was investigated using the RT-PCR method.

The measured IC50 concentration of the ginger extract was 20 mg/ml. Determination of the expression of the COX-2 gene using the RT-PCR method indicated that the ginger extract at 20 mg/ml reduced expression of the COX-2 gene compared to using Aspirin. Therefore, ginger extract could be used as an anti-inflammatory drug against the HT-29 cells of colon cancer.  Reduction of inflammation is important in patients with colon cancer, and results of this research indicated use of ginger extract could play an important role in decreasing inflammation and in reducing the expression of the COX-2 gene, which has a significant role in the synthesis of prostaglandins and in causing inflammation.

Antioxidants are compounds that eliminate free radicals, which reduce tissue damage and allow the organs and blood vessels to properly heal. In this research, the anticancer effect against MCF-7 and HT-29 cell lines, and antioxidant, antimicrobial and wound healing activities of the lichens extracts have been investigated.

Anticancer activity was assayed with standard MTT colorimetric procedure against MCF-7 and HT-29 cell lines. Antioxidant activity was studied by measuring DPPH, reducing power, total phenols and flavonoids assays. In vivo antimicrobial property and gas chromatography mass spectrometry analysis were evaluated.

Metanolic extract of Umbilicaria decussata showed nearly 50 % HT-29 cell line inhibition at 200 μg/mL tested dose. Protoparmeliopsis muralis extract showed stronger antibacterial activity (MIC= 125 mg/mL) than Fulgensia fulgens. Also, it had a largest free radical scavenging activity (65.67%). The results revealed that there was a strong positive correlation between flavonoids and total phenolics (r= 0.952). In excision wound model, there was a significant reduction in both wound surface area and bacterial colony count in F. fulgens and especially P. muralis methanolic extracts. These significant activities are found due to the presence of unique compounds as Usnic acid, 8S,14-Cedrandiol and 3,9-Dimethyltricyclo[4.2.1.1(2,5)] dec-3-en-9-ol in P. muralis and 3-[5-(2-Chloro-5-nitro-phenyl)-furan-2-yl]-2-cyano-acrylic acid and 3-[2,4-Dichlorophenyl]-1-hydroxy-9,10-anthracenedione in F. fulgens.

These results show that compounds as 3-[5-(2-Chloro-5-nitro-phenyl)-furan-2-yl]-2-cyano-acrylic acid, 3-[2,4-Dichlorophenyl]-1-hydroxy-9,10-anthracenedione and usnic acid are responsible for accelerating the wound healing process by antimicrobial and antioxidant activities in these studied species.