جستجوی مقالات مرتبط با کلیدواژه « human papillomavirus (hpv) » در نشریات گروه « پزشکی »

Cervical cancer global burden is highly skewed towards poor countries primarily due to lack of awareness, poor screening, and low uptake of prophylactic vaccines. The purpose of our study is to educate and raise awareness among young girls and women about the importance of cervical screening and HPV vaccination.

The present study, conducted from January 2023 to December 2023, focused on students, teachers, housewives, and healthcare professionals in the Jammu region to assess their awareness of cervical cancer and the HPV vac- cine. HPV DNA testing was carried out using the Truenat Real-Time PCR method at Swastik Diagnostic Laboratory, Jammu.

Knowledge of cervical cancer, awareness of the HPV virus, and the vaccination status of women were assessed in survey. In the HPV screening test, out of 2,400 women, 106 tested positive for HPV. Among these 106 women, 19% had a high viral load (Ct < 20), 11% had a low viral load (25 ≤ Ct < 30), indicating a low relative concentration of HPV viruses, 40% had a medium viral load (20 ≤ Ct < 25), and 30% had very low viral loads (Ct ≥ 30).

These findings highlight the importance of routine cervical screenings, such as Pap smears and HPV tests, for the early detection of cervical cancer. There is an urgent need to implement cervical cancer screening and vaccination pro- grams in the Jammu region.

Cervical cancer stands out as one of the most prevalent gynecological cancers. Cervical cancer’s link to human papillomavirus (HPV) testing and recurrence is unclear despite previous studies. Investigating this relationship in Iranian patients is a pivotal aspect of this research.

This study encompassed all cervical cancer patients referred to Firoozgar Hospital, Tehran, Iran, between 2016 and 2018. Utilizing a census method, the patients’ data, including their demographics and treatment details, were extracted from records. Follow-up samples were collected after vaginal cuff or cervix treatment (surgical or radiotherapy). A total of 124 patients were included in the study and categorized into two groups: Patients with recurrence and those without. INNO-LiPA standard test was employed to detect HPV presence. Comparative analysis of various variables, such as age at diagnosis, smoking history, multiple partners, sexually transmitted diseases history, body mass index, abnormal cervix, vaginal fornix involvement, cervical parameters involvement, tumor size, The International Federation of Gynaecology and Obstetrics (FIGO) staging, MRI staging, pathology, and treatment, was conducted between the recurrence and non-recurrence groups.

A total of 124 patients were included in the study, with an average age of 45.95±7.45 years. Most patients had an 18-25 kg/m2 body mass index. Based on MRI findings, the most common stage of cervical cancer was stage III, IIB. Squamous cell carcinoma pathology was the most prevalent, observed in 47 patients (37.9%). The most frequently performed treatment modality was radical trachelectomy and adjuvant chemoradiotherapy, accounting for 20 cases (16.1%). There were no significant differences in terms of age at disease diagnosis, smoking history, body mass index, histological type, and treatment type between patients with recurrent disease and those without recurrence. However, a significant difference was observed in terms of lymph node involvement. In addition, the HPV test was positive in 3 cases (2.4%) with recurrence and 11(8.9%) without. These results suggest no relationship between hrHPV (high-risk human papillomavirus) status and disease recurrence (P=0.196).

The result of our study showed no correlation between positive HPV test and cervical cancer recurrence. Consequently, HPV testing is not suitable as a reliable predictor for the recurrence of cervical cancer.

Developing potent therapeutic vaccines against human papillomaviruses (HPVs) is crucial for the effective management of various HPV-associated cancers. DNA-based vaccines are attractive due to their safety, stability, and capacity to elicit a targeted immune response against specific antigens. Heat shock proteins (HSPs) can enhance the efficacy of DNA vaccines when used as adjuvants. In this study, we created a recombinant DNA molecule by fusing the HPV16 e7 gene with either the hspB1 or hsp27 gene and assessed its expression in a eukaryotic cell line.

Initially, we constructed a recombinant eukaryotic expression vector by inserting the hsp27-e7 fusion gene into the pcDNA3.1 (-) vector. The concentration and purity of the sample were evaluated using NanoDrop spectrophotometry. We cultured human embryonic kidney 293T (HEK-293T) cells in RPMI 1640 medium and transfected them with the pcDNA3.1-hsp27-e7 construct using Lipofectamine 2000 transfection reagent. After 48 hours, we assessed the expression of the Hsp27-E7 fusion protein by western blotting using an anti-E7 monoclonal antibody.

We successfully subcloned the hsp27-e7 fusion gene into the pcDNA3.1 (-) vector, and enzymatic digestion confirmed a distinct ~975 bp band on an agarose gel. The concentration and purity of the recombinant DNA vector in a 10 mL culture were measured to be 210 ng/µL and 1.86, respectively. Furthermore, the expression of the Hsp27-E7 fusion protein in HEK-293T cells was confirmed by Western blot analysis, which detected a distinct band of approximately 38 kDa.

Our in vitro findings demonstrate successful expression of the DNA construct encoding the hsp27-e7 gene, which can be utilized as a DNA vaccine for future in vivo investigations.

Human papillomavirus (HPV) is considered to be the important viral agent associated with several human cancers, the most important of which is cervical cancer. Today, the role of this virus in gastrointestinal cancers, including gastric cancer (GC), has also been considered. This study performed to clarify the possible association of HPV and the occurrence of GC in Ardabil province, Northwest Iran, which is a high-risk area.

The study involved 140 paraffin-embedded specimens of gastric tissue that divided into two groups based on the pathological diagnosis: 70 patients with GC as the case group and 70 samples without a diagnosed tumor in gastric tissue as control. The nested polymerase chain reaction (Nested-PCR) method was carried out to detect the HPV genome in paraffin embedded gastric tissue samples. Finally, samples that were positive for the presence of the HPV genome were sequenced to determine the type of virus.

HPV genome was detected in 33 (47.14%) of 70 gastric cancer samples and 4.28% (3/70) of samples without gastric cancer. In case and control groups 97% and 67% of HPV positive samples were over 40 years old, respectively and the number of men was more than women. Ultimately, HPV-16 and HPV-18 were detected in PCR positive samples by sequence analysis.

Based on our founding, the infection rate of HPV in patients with gastric cancer was significantly higher than that in non-cancerous samples of gastric tissue. Moreover, high-risk types of HPV (16, 18) were detected in all positive samples. Therefore, the results of this investigation suggest that HPV can be one of the possible risk factors for the occurrence of gastric cancer in Ardabil province.

Prostate cancer (PCa), as the fifth leading cause of death, is the second most common cancer diagnosis in men worldwide. Human papillomavirus (HPV) can potentially contribute to PCa development and chronic inflammation. HPV infection leads to malignant and benign lesions in the genital areas of men and women. The data on the role of HPV in PCa development is contradictory. 

This study aims to investigate the frequency of HPV in PCa samples in hospitals in Tabriz City, Iran.

This study was conducted in a cross-sectional descriptive manner. Paraffin tissue blocks including 50 patients with primary prostate adenocarcinoma and 50 patients with benign prostatic hyperplasia (BPH) were selected from Tabriz hospitals. All samples were examined for the presence of HPV16/18 by the polymerase chain reaction (PCR) method.

3 out of 50 PCa patients are infected with HPV18. None of the benign and malignant prostate samples are infected with HPV 16. Therefore, in our study, no connection exists between HPV and PCa. The Mean±SD age of HPV positive samples was 61.33±11.50 years.

The result of this research does not support the role of HPV in the development of PCa. Therefore, additional studies are necessary to clarify the possible role of HPV in prostate carcinogenesis.

Colorectal cancer (CRC) is the third common cause of malignancy in Iran, and its incidence rate has been raised due to an increase in individual and environmental risk factors.

The present study aimed to define the role of human papillomavirus (HPV) infection in CRC, then by drawing a phylogenetic tree, we aimed to analysis the sequences of isolated HPVs, and finally determine the viral genome physical status for HPV-16.

A nested-PCR (nPCR), direct sequencing, and INNO-LiPA HPV genotyping assay were carried out for HPV detection, and a quantitative real-time PCR assay (qRT-PCR) was conducted for the physical status of the viral genome.

Among 157 subsets, there were 66 cases and 91 controls. Moreover, 5.7% (9/157) were HPV positive by nPCR, among whom 9.1% (6/66) were cases and 3.3% (3/91) were control groups (P = 0.750). The qRT-PCR analysis showed three forms of integrated, episomal, and mixed forms in our three HPV-16 strains. A higher rate of HPV infection was detected in the CRC cases; however, it was not statistically significant.

Although there were limited positive results, higher ages (> 65) were related to HPV involvement and CRC outcome. Furthermore, 9.1% of the CRC cases were infected with HPVs. The HPV-16 genome integration is observed in high-grade cancer cases with severe malignancy in CRC patients.

Human papillomavirus (HPV) is recognized as a most important risk factor in oral cavity cancer and pre-malignant lesions; however, etiological association of concomitant infection with other oncogenic viruses as a co-factor has not been definitively proven. The present study aimed to determine the prevalence of co-infection with HPV, Epstein–Barr virus (EBV) and Merkel Cell PolyomaVirus (MCPyV) in oral cavity lesions in Iranian patients. One hundred and fourteen oral cavity samples, including 33 oral squamous cell carcinoma, 28 oral lichen planus, 16 oral epithelial dysplasia and 37 oral irritation fibromas were analyzed for the HPV, EBV and MCPyV infection by quantitative real-time PCR. According to histological features 32.5% and 28.9% of cases were oral irritation fibroma and oral squamous cell carcinoma, respectively. Infection with at least two viruses was detected in 21.1% of patients. In this group, co-infection with HPV/EBV was identified in 37.5% of cases, HPV/MCPyV in 29.2%, EBV/MCPyV in 12.5%, and HPV/EBV/MCPyV in 20.8%. There was no statistically significant difference between multiple infections and anatomical locations of cancer. The prevalence of triple viral infection (HPV/EBV/MCPyV) in well differentiated tumors was higher than EBV or MCPyV single infection. This study revealed that co-infection of HPV, EBV and MCPyV can be detected in both malignant and non-malignant oral cavity tissues, and co-infection with all three viruses in well differentiated tumors can be shown as a synergistic hypothesis of the pathogenic role of these viruses in oral malignant transformation.

Persistent infection with some types of Human papillomavirus (HPV), which are high-risk genotypes, can lead the patients toward cervical cancer and, finally, death. Recent studies showed HPV co-infection with non-HPV sexually transmitted infections (non-HPV STIs) could increase the persistency rate of HPV infections. This study aimed to estimate the prevalence of STIs and assess the association of HPV/non-HPV STIs co-infection on cervical cell changes based on cytological findings.

In this cross-sectional study, in addition to the routine cervical screening, including HPV testing and cytological assessment, non-HPV STI testing was performed on 1065 Iranian women. To assess the HPV and non-HPV STIs, commercial kits were used.

964 (90.5%) women had normal cytology (NILM) results. The overall prevalence of HPV and non-HPV STIs were 39.1% and 68.5%, respectively. HPV-53 (6.5%), -16 (6.1%) and -31 (5.5%) were found as the most prevalent genotypes. Ureaplasma Parvum (UP) (42.7%), Group B Streptococcus (GBS) (23.7%), Candida Species (CS) (23.6%), Ureaplasma Urealyticum (UU) (9.6%), and Mycoplasma Hominis (MH) (7.1%) were found as the most prevalent non-HPV STIs. The co-infection of HPV with GBS played an important role in developing the cervical lesion (P<0.05).

In the present study, the STIs, including HPV, UP, GBS, CS, UU, and MH, were prevalent among the study participant, and it was found that the HPV/GBS co-infection played a significant role in the development of LSIL or worse cytological grades. To clarify this issue, further studies will be conducted.

Focal epithelial hyperplasia (FEH), also known as Heck’s disease, is a human papilloma virus-induced proliferation of epithelial cells, which causes asymptomatic lesions in the oral mucosa. Heck’s disease is more prevalent among children or in a family. Despite the self-limitation of lesions, they may progress or cause esthetical and functional discomforts. Hence, early diagnosis is necessary.

A middle-aged man with peripheral multiple soft, elevated, sessile, smooth, lobulated surface and nodular lesions in his right buccal mucosa was referred. The dimensions of the lesions varied from 5 to 25 mm. Through clinical and histopathological examinations, Heck’s disease was diagnosed. The diode laser (wavelengths of 980 nm), 1-W power using continuous waves) was applied serially (in 2 sessions with a 1-week interval) for surgically removing the lesions. The patient was followed up for 6 months with no lesion recurrence, and gradual healing was observed.

The laser is an effective treatment with no scar remaining after the procedure and brings satisfaction to patients.

The human papillomavirus (HPV) is associated with more than 70% of the cervical neoplasm. The current study aims to evaluate the distribution of HPV genotypes in suspected women cytological specimens from Tehran, Iran.

In the current cross-sectional study, HPV genotype prevalence was investigated in 433 subject women. DNA extraction was performed by High Pure Viral Nucleic Acid kit. A semi-automatically hybriSpot 24™ (HS24) setting was used for HPV typing and data interpreted by hybriSoft™ software according to instructions.

Pathologic data showed 181 (41.8%) had non-malignant lesions, 212 (49%) had inflammation and 40 (9.2%) reported LSIL in primary Pap-smear result. HPV was found in 143 (33%) specimens and the most comment high-risk and low-risk HPV types were HPV-16 and -6, respectively. Also, 62 (43%) were co-infected with multiple genotypes includes, 34 (24%) cases had co-infection with two HPV types, 17 (12%) cases had co-infection with three HPV types, 6 (4%) cases had co-infection with four HPV types and 5 (3%) cases had co-infection with five HPV types. There was statistically different domination on high-risk genotype in most of the co-infected samples (p<0.01).

Current study indicates that the lesion pathology assessment was significantly associated with the HPV infection (p<0.01). Furthermore, the age group assessment shows that most of the HPV positive cases were 21 to 40 (p<0.01). The HPV infection prevalence in the current study was 33% and the most frequently reported high-risk and low-risk HPV types were 16 and 6, respectively.

 Recent advances in molecular testing for human papillomavirus (HPV) has increased the accuracy of cervical screening programs. This study intended to estimate the diagnostic value of high-risk (HR) HPV DNA- and E6/E7 mRNA-based methods for triage of Iranian women with abnormal cytological results regarding the histopathological cut-off.

In this cross-sectional study, 360 non-pregnant women (≥ 21 years) who had faced abnormal cytological findings (ASC-US and LSIL) were enrolled and referred for further diagnostic tests. The INNO-LiPA® HPV Genotyping Extra-II and Aptima HPV assay kits were used in DNA- and E6/E7 mRNA-based methods for detection of HR-HPV. Regarding the CIN-2+ histopathological cut-off, the diagnostic value of each molecular-based assay was calculated.

Among the study participants, 260 cases had ASC-US, and 100 had LSIL. The overall positivity rate for DNA- and mRNA-based methods was 74.4% (268/360) and 64.2% (231/360), respectively. Fifty-nine (16.4%) individuals showed CIN-2+. The DNA-based test showed higher sensitivity (100%) than the mRNA-based method (93.2%), while the mRNA-based method revealed greater clinical specificity (41.5%) compared to the DNA-based test (30.6%).

Our results revealed appropriate clinical sensitivity of the molecular-based methods for triage of Iranian women with abnormal cytological results; however, the mRNA-based method showed greater specificity for detection of CIN-2+.

 Prostate cancer is one of the most frequent cancers in men, with several risk factors, including infection. In this study, we aimed to assess the association of human papillomavirus (HPV) infection with prostate cancer risk and aggressiveness.

 This case-control study was performed on a total of 70 archival formalin-fixed paraffin-embedded (FFPE) prostatic tissue blocks. Among them, 35 histopathologically confirmed prostate cancer tissues and 35 benign prostate hyperplasia (BPH) samples were enrolled as cases and controls, respectively. The specimens were examined by the polymerase chain reaction (PCR) and in situ hybridization for the detection of HPV DNA of both low-risk (6, 11) and high-risk (16, 18) types.

 The primary results of PCR for the L1 region revealed HPV infection in 34.3% and 8.6% of the cases and controls, respectively (P = 0.018). The HPV typing using hybridization revealed a significant difference between the two groups in terms of infection with HPV types 16 and 18 (P = 0.003, P = 0.028, respectively). The findings showed no significant association of HPV infection with age and prostate specific antigen (PSA) level of the patients. In addition, no significant association was found between infection with HPV and Gleason score (GS).

 Our findings indicated that HPV infection, especially with high-risk types 16 and 18, has a significant association with prostatic carcinogenesis. In addition, although patients with GS = 7 showed higher rates of infection with HPV, we did not observe any statistical association between HPV infection and GS.

The purpose of this study was to investigate the association between IL-4 -589C>T, IL-6 -174G>C, IL-1β -31T>C and IL-1β -511C>T genes polymorphism with high concentrations of human papillomavirus (HPV), and the influence of gene-gene interactions on persistent human papillomavirus infection.

In this study, 101 infected women with high HPV viral load and 93 healthy women were involved in a case-control study. Genotyping of SNPs for IL-4 -589C>T, IL-6 -174G>C, IL-1β -31T>C and IL-1β -511C>T genes was carried out by allele-specific PCR. Quantitative analysis of HPV-DNA was performed by Amplisens HPV HCR genotype-titer software. Gene-gene interactions were analyzed using multifactor dimensionality reduction (MDR) algorithm. Haplotype interactions were analyzed by HaploView 4.2 tool.

The study of single individual SNPs in promoters of IL-4 -589C>T, IL-6 -174G>C and IL-1β -31T>C genes did not reveal statistically significant difference in genotypes and allele frequencies among women with high HPV viral load and control group. The frequency of -511T allele and TT genotype of the IL-1β gene in case group was significantly higher than the one in control group (OR=1.71, p=0.012 and OR=2.02, p=0.046, respectively). Haplotype analysis revealed that -511C/-31T haplotype for IL-1β gene is significantly less common among women with high HPV viral load (p=0.018).

The haplotype -511C/-31T for IL-1β gene is associated with a protective effect against increasing HPV viral load. The frequencies of -511T allele and -511TT genotype of the IL-1β -511C>T were significantly higher among women with HPV in comparison to control group.

Human papillomavirus (HPV) has been found as the most considerable causes of cervical cancer. Recently, several molecular methods have been introduced to increase the accuracy of the screening programs and decrease the mortality rate. Among these methods, mRNA-based methods have more advantages as they assess the expression level of HPV E6 and E7 oncogenic mRNAs. This study aimed to evaluate the results of HPV RNA- and DNA-based methods among Iranian women population with normal cytology results.

Overall, 4640 women were enrolled referred to the Gynecology Oncology Ward of Vali-e-Asr Hospital, private and academic clinics, Tehran, Iran from Jan 2016 to Apr 2018. To assess the HPV-DNA infection INNO-LiPA® HPV Genotyping Extra-II kit was used. For HPV-RNA assessment, Aptima HPV Assay and in house HPV-RNA genotyping methods were applied.

The positivity rates of HPV infection according to DNA- and RNA-based methods were 18.0% and 11.2%, respectively (P<0.001). The positive predictive value, negative predictive value, specificity and sensitivity of DNA-based method in contrast with RNA-based method were 59.2% (56.6-61.6), 99.4% (99.0-99.6), 91.7% (90.8-92.6) and 95.2% (93.0-96.9) respectively.

At the present study for prognosis of cervical cancer, RNA-based method seemed to be more specific in contrast to DNA-based method. Patient follow up and further studies will be conducted in order to clarify the clinical sensitivity and specificity of the two methods.

 Esophageal cancer (EC) is a common cancer worldwide. Despite epidemiological studies, the etiology of EC is undetected. It was recommended that tobacco, alcohol, food carcinogens, and infectious agents may be involved in the pathogenesis of EC. Accumulating evidence suggests the role of human papillomavirus (HPV) on EC.

 The aim of this study was to evaluate the prevalence and association of HPV with EC.

 In this case-control study, 86 samples of formalin-fixed and paraffin-embedded esophageal tissues were gathered from the pathology laboratory, Imam Khomeini Hospital, Urmia, West Azerbaijan, Iran. A total of 43 samples were esophageal cancers (cases) and 43 samples were esophageal non-cancerous tissues (controls). The tissues were sectioned and deparaffinized, and deoxyribonucleic acid was extracted. The polymerase chain reaction test was conducted to detect HPV, using general (GP5+/GP6+) and type-specific (E6/E7) primers. HPV types were confirmed by sequencing. The data were analyzed by the SPSS software.

 Out of 86 samples, 23 (26.74%) were positive for HPV (15 cases, and 8 controls). By type-specific primers, high-risk HPVs were detected in the cases (3 HPV-16, 10 HPV-18, 1 HPV-31, and 1 HPV-33) and the controls (6 HPV-18, 1 HPV-31, and 1 HPV-33). No significant association was observed between HPV and EC (P = 0.078).

 Although no significant association was observed between HPV and EC, high-risk HPV genotypes were found in esophageal cancers more than non-cancerous esophageal tissues. To confirm this result, more studies should be carried out in other populations.