جستجوی مقالات مرتبط با کلیدواژه "human polyomavirus" در نشریات گروه "پزشکی"

 Human polyomavirus BK virus (BKV) belongs to the Polyomaviridae family and seems to be a drastic virus in prostate cancer (PCa) etiology. BK virus induces oncogenesis via the expression of large tumor antigen (LTAg) and small tumor antigen (stAg). Also, BKV infection seems to play an essential role in prostate cancer development.

 In this study was aimed to study the prevalence of BKV in benign and cancerous prostate tissues.

 In this study, 100 formalin-fixed paraffin-embedded tissues of PCa specimens and benign prostatic hyperplasia (BPH) were collected. The DNA was extracted from tissue samples, and the BKV DNA was investigated using a semi-nested polymerase chain reaction (PCR). The MEGA 6.0 software was used for phylogenetic analysis to assemble the viral genome. A phylogenetic tree was constructed by neighbor-joining analysis with 1,000 replicates of the bootstrap resampling test.

 The BKV DNA was found in 66% (33/50) of patients with PCa and 36% (18/50) of patients with benign prostatic hyperplasia (BPH) (P = 0.003). The frequency of BKV DNA in different classes of Gleason score (5 - 10) was not significant (0.094). The distribution of BKV DNA among different age groups was not significant (P = 0.086).

 High frequency of BKV infection was detected in patients with PCa compared to patients with BPH (P = 0.003), and the coexistence of BKV DNA was confirmed in 51% (51/100) of tissue samples, which were confirmed to be subtype 1 of BKV infection.
 

Systemic lupus erythematosus (SLE) is an autoimmune disease and human polyomavirusBK (BKV) can be reactivated in patients with SLE due to the changes in the immune system and use of immunosuppressive drugs.In this study, we evaluatedthe prevalence of BKV infection among patients with SLE referred to Golestan hospital in Ahvaz, Iran betweenApril 2013 to June 2016.

In this cross-sectional study we studied 75 individuals including 40 patients with SLE and 35 normal individuals. Urine and blood samples were taken and DNA was extracted from urine and plasma. Polymerase Chain Reaction (PCR) test was used to detect the BKV genome and positive samples were sequenced to confirm BKV. BioEdit software and MEGA 6.0 software were used for phylogenetic analysis to assemble the viral genome. A phylogenetic tree was constructed by neighbor-joining analysis with 1,000 replicates of the bootstrap resampling test using Mega 6.0. Statistical analysis was done by SPSS version 22.

Among the 40 patients, 2(5%) were men and 38(95%) were women. The mean age of the patients was 39±10 years. 2.5% of plasma from patients with SLE were positive for BKV but none of the controls were positive in this regard.0% of control groups (p=0.346). Whereas in urine samples, 17.5% and 11.4% (p=0.458) of patients and the control group, were positive for BKV, respectively. However, there was no statistically significant difference between the patients and controls.

BKV reactivation occurs in 17.5% of patients with SLE during immunosuppression therapy. Therefore, more studies on BKV DNA by highly sensitive molecular assays in Patients with SLE seem to be necessary.

The role of BK and JC polyomaviruses (BKV and JCV) in the causation of respiratory disease and the natural route of transmission has not well been established. The aim of study was to determine the prevalence of BK and JC viruses in 280 respiratory samples and evaluate their contribution to respiratory disease.

PCR was used to screen specimens for BKV and JCV and either single or multiplex RT-PCR, or real time PCR was used to determine co-infection with other viruses. Positive results were confirmed with sequencing.

Of the 280 samples analysed, eight (2.85 %) were positive for BKV. BKV positive samples were from immunocompetent (n=5; 1.78%) and immunocompromised patients (n=3; 1.07%). The positive samples in the immunocompetent group were patients age range 8 days to 29 years. In the immunocompromised group, BKV positive patients age range 30 years to 69 years. Co-infections were found in 3 (37.5%) of the BKV positive samples. No sample was found to be positive for JCV.

Detection of BKV DNA in respiratory specimens supports previous studies suggesting the respiratory tract may be the primary site for acquisition or infection by BK virus at an early age and also reflect the reactivation of latent or persistent infection with the virus. Respiratory tract may not be considered as a site for JC viral persistent infection.