جستجوی مقالات مرتبط با کلیدواژه "inva" در نشریات گروه "پزشکی"

Salmonellosis is among the most common food-born infections, caused by Salmonella spp. bacteria. Present study has investigated the frequency and antibiotic resistance pattern of Salmonella spp. isolated from traditional dairy products and raw milk supplied in Yazd, Iran.

In a cross-sectional study, 350 samples of raw milk and traditional dairy products were randomly collected from July to September 2018. Following culturing the samples, isolates went through biochemical tests for phenotypic identification. Results were confirmed through PCR technique by targeting invA gene. Antimicrobial susceptibility test was conducted by means of disk diffusion method.

The rate of contamination with Salmonella bacteria was 6.57% in all samples. The PCR assay of all isolates showed that 23 isolates (100%) carried the invA gene. No significant association between the frequency of salmonella spp. and types of dairy and their origin was reported (P>0.05). The highest antibiotic resistance rate among the isolates belonged to tetracycline (34.8%) and the highest sensitivity was seen to imipenem, cefepime, and cefotaxime (each 91.3%).

According to our results there has been a rise in multiple drug resistance and contamination rate in traditional dairy products in Yazd province.

Foodborne diseases caused by Salmonella are considered as a global health concern, especially in low-income countries. Rapid and specific detection of this infective agent is highly important in the outbreak control. The current study aimed to design and optimize a LAMP method and to compare its sensitivity and efficiency with the PCR method in the detection of S. typhi in food.

Food samples including mayonnaise and vegetable salad were inoculated with S. enterica serovar Typhi. Sensitivity and detection limit of LAMP test were investigated at different concentrations of contaminated mayonnaise and vegetable salad. invA gene was chosen as the target gene for bacterial detection by PCR and LAMP tests.

The detection limit of Samonella was estimated to be 16 CFU/mL using LAMP and PCR. LAMP reaction revealed a visible turbidity, indicating the accurate amplification of the selected target gene and proper identification of Salmonella at different dilutions of the studied food samples.

The present study indicated that LAMP is a rapid, cost-effective, and specific technique for the identification of Salmonella. This method could be used in laboratories with minimal equipment without the need for costly molecular detection methods.