جستجوی مقالات مرتبط با کلیدواژه "l-ascorbic acid" در نشریات گروه "پزشکی"

Vitamin C and long-pulsed Laser Nd:YAG 1064 nm can reduce the severity of acne scars by increasing the number and density of collagen fibers. Furthermore, applying vitamin C after the skin resurfaces can improve its penetration into the skin. This study aimed to prove the difference between the effectiveness of the long-pulsed Nd:YAG 1064 nm laser with 15% vitamin C (L-ascorbic acid) solution combination therapy and isolated long-pulsed Nd: YAG 1064 nm laser therapy in atrophic acne scars by assessing the decrease in Goodman and Baron scores. This study took the form of a double-blind, randomized controlled trial using a pretest-posttest comparison group design on patients with atrophy acne scars on the face. Both groups were assessed using the Goodman and Baron scores before and after therapy for three months. Subsequently, descriptive analysis andhypothesis testing were performed. In the treatment group, the mean Goodman and Baron score decreased significantly after treatment to 13.9 ± 7.39 (P = 0.008), while in the control group, the mean score decreased significantly to 18.2 ± 9.34 (P = 0.007). Goodman and Baron Delta scores in the treatment group were significantly higher at 7.3 ± 2.78 compared with the control group at 3.1 ± 1.05 (P < 0.001). Long-pulsed Nd:YAG 1064 nm laser with 15% vitamin C solution (L-ascorbic acid) combination therapy is more effective in lowering the Goodman and Baron scores for atrophic acne scars than isolated long-pulsed Nd:YAG 1064 nm laser.

Induced pluripotent stem cells (iPSCs) can be generated from different source of cells with different efficiencies. Two DNA methyltransferases DNMT1 and DNMT3A have been shown to regulate epigenetically the gene expression involved in cell viability and reprogramming. L-ascorbic acid (L-AA) is a chemical factor that can accelerate reprogramming. Here, we sought to investigate the effect of L-AA on DNMT1 and DNMT3A expressions.

First, mouse embryonic fibroblasts at passage 3 were cultured in the presence of 10 μg/ml L-AA days for 5 days. Then, DNMT1 and DNMT3A expressions were determined using real-time PCR at days 3 and 5.

It was showed that L-AA could enhance DNMT-1 expression which involve in cell viability and decrease the DNMT3A which involve in cell differentiation.

The results therefore suggest a new insight into L-AA mechanism impact on reprogramming process