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جستجوی مقالات مرتبط با کلیدواژه « leuconostoc.mesenteroides » در نشریات گروه « پزشکی »

  • Anik Ma'unatin*, Harijono Harijono, Elok Zubaidah, Muhaimin Rifa'i
    Background and Objectives

    Lontar (Borassus flabellifer L.) is widely grown in Indonesia and one of its products is palm sap. Palm sap contains a high level of sugar, making it suitable as a medium to increase the lactic acid bacteria (LAB) production of exopolysaccharides (EPS). This study aimed to isolate the EPS-producing LAB from palm sap and evaluate its EPS production. LAB isolation was carried out on MRS agar containing 0.5% CaCO3.

    Materials and Methods

    The screening and production of EPS were carried out on MRS media supplemented with 10% sucrose. The molecular identification of the selected EPS-producing LAB was based on 16S rDNA. A quantitative analysis of EPS polymer dry mass and total sugar was conducted using one-way ANOVA.

    Results

    In this study, five EPS-producing LABs were found: Fructobacillus fructosus N4, Leuconostoc mesenteroides N5, Leuconostoc mesenteroides N7, Leuconostoc mesenteroides N9, and Fructobacillus fructosus N10. The highest EPS yield in liquid media was 10.997 ± 1.591 g/L by Leuconostoc mesenteroides N7, whereas the lowest was 4.505 ± 0.459 g/L by Fructobacillus fructosus N10.

    Conclusion

    This study found Fructobacillus fructosus strains as EPS producers that have never been reported before.

    Keywords: Borassus flabellifer, Exopolysaccharide, Fructobacillus fructosus, Lactic acid bacteria, Leuconostoc mesenteroides}
  • یوسف رحیمی کشکولی، مهدی فرامرزی، حمیدرضا رحیمی کشکولی، داریوش غلامزاده
    زمینه و هدف
    دکستران پلی ساکاریدی متشکل از مونومرهای گلوکز می باشد که به طور گسترده به عنوان توسعه دهنده حجم خون در داروسازی استفاده می شود. هدف این مطالعه تولید دکستران از ملاس نیشکر با استفاده از باکتری لوکونوستوک مزانتروئیدس بود.
    روش بررسی
    در این مطالعه تجربی، جهت رشد میکروارگانیسم و تولید دکستران، ملاس نیشکر در غلظت های مختلف به محیط کشت اضافه شد. رسوب دکستران بعد از 48 ساعت با اضافه کردن اتانول، تکان دادن و سانتریفوژ کردن حاصل گردید. جهت شناسایی کیفی، از اثر دکستران بر پولاریزاسیون و برای طراحی و تحلیل داده ها از روش آماری طرح رویه پاسخ استفاده شد.
    یافته ها
    پس از بررسی اثر جداگانه و متقابل پارامترها بر مقدار دکستران تولیدی نتایج مربوط به سطوح بهینه به صورت 50 گرم بر لیتر ملاس نیشکر، دمای 35 درجه سانتی گراد و 5/8 = pH به دست آمد که در این شرایط، بیشترین دکستران برابر با 82 گرم بر لیتر تولید شد. ضریب همبستگی مدل محاسباتی برای دکستران تولیدی 5/99 درصد به دست آمد که بیانگر تطابق عالی مدل محاسباتی با نقاط آزمایش شده و دقت بالای مدل بود.
    نتیجه گیری
    تولید دکستران به وسیله باکتری لوکونوستوک مزانتروئیدس و ملاس نیشکر به عنوان سوبسترا یک روش کم هزینه و مقرون به صرفه در مقایسه با روش های رایج تولید دکستران می باشد و علاوه بر تولید یک محصول کلینیکی، بار آلایندگی ملاس در محیط زیست به طرز چشمگیری کاهش می یابد.
    کلید واژگان: دکستران, ملاس, لوکونوستوک مزانتروئیدس}
    Background and Aim
    Dextran is a polysaccharide consisting of glucose monomers that are widely used in medicine as a blood volume extender. The aim of this study was to produce dextran from cane molasses using Leuconostoc mesenteroides bacteria.
    Methods
    In this experimental study، for bacterial growth and dextran production، sugarcane molasses was added to the culture medium at different concentrations. Dextran sedimentation was obtained by shaking and centrifugation by adding ethanol after 48 hours. Response surface design was used for qualitative identification of the polarization of dextran and statistical analysis methods.
    Results
    After assessing the separation and interactive effects of the parameters on the optimum amount of dextran produced from sugarcane molasses as 50 g، 35 º C and 5/8 = pH، the Dextran produced was more than 82 g/l. The correlation of the computational model for the dextran produced was 99. 5%، which indicated excellent agreement with the experimental and computational models of high accuracy.
    Conclusion
    Dextran produced by Leuconostoc mesenteroides bacteria and sugarcane molasses as substrate، is a cheap and affordable compared to current methods of dextran production. In addition to producing a clinical product، the molasses pollution could be dramatically decreased.
    Keywords: Dextran, Molasses, Leuconostoc Mesenteroides}
  • J. Mohammad Hassani *, Sh. Darvishi, S. E. Hosseni, F. Mirahmadi
    Introduction
    The use of nitrogen fertilizers and low enzyme activity of nitrite and nitrate reductase in leaves of plants, causes the accumulation of nitrite and nitrate in leafy plants such as white cabbage (B.o var. alba).Nitrite is a highly carcinogenic substance therefore in this study changes in nitrite concentration during fermentation of fermented cabbage and spontaneous fermentation by inoculation of culture were examined.
    Materials And Methods
    The strains used in fermented cabbage were Leu.meseneteriodes, subsp.mesenteroids PTCC 1563 and L. plantarum PTCC 1058 (Organization of Scientific and Industrial Research of Iran).The strains and their mixture as active culture, with equal numbers 5.6×106 cfu/gr were selected. Cabbage treatments consisted of inoculation with pure lactic starter (hemofermantative), inoculation with pure lactic starter (hetrofermantative), inoculation with a starter mix and cabbage not inoculated (control) were performed.
    Results
    Significant differences were recorded by the mean values of residual nitrites, pH, total number of lactic acid bacteria in the control samples (not inoculated) and inoculated samples (P<0.05).Residual nitrites in fermented cabbages by L. plantarum, Leu. mesentroides and mixed cultures were reduced up to 67.7%, 68.9%, 84.5% respectively, while it was 51.9% in the control treatment.
    Conclusion
    The best treatment concering residual nitrite was the fermented cabbage by mixed culture. The best treatment concering the pH and the acidity levels was the cabbage inoculated by Leu. mesentroides.
    Keywords: Leuconostoc.mesenteroides, Lactobacillus.plantarum, Nitrite, Sauerkraut}
  • M. Fathiazar *, S. M. Seyedain Ardabili
    Introduction
    The object of this study was to employ sourdough containing specific starter cultures and apply it for the production of Barbari bread and compare the quality of the product with the traditional Barbari bread which is produced in Iran.
    Materials And Methods
    In this work four treatments of sourdough were prepared. In the first treatment as a control group, baker’s yeast (B1) was used. A mixture of Lactococcus lactis, Leuconostoc mesenteroides bacteria and baker’s yeast (B2), a mixture of Lactococcus lactis, Leuconostoc mesenteroides and the yeast Kluyveromyces marxianus (B3) and finally a mixture of Lactococcus lactis, Leuconostoc mesenteroides bacteria (B4) were used as second, third and fourth treatments.
    Results
    The quality evaluations of the texture of the samples were performed by application of Texture Profile Analyser (TPA) after 1, 2 and 3 days and the sensory evaluations were conducted after 1 day. The sensory evaluations revealed that there were not significant differences between B1, B2, and B4 groups while these groups had significant differences with B3 group in terms of appearance and crust. The B1 and B2 samples showed better porosity, chewiness and texture softness than the B3 and B4 samples. There was no significant difference (p0.05) among the samples in term of odor and taste. The TPA analysis revealed that B2 and B3 samples were softer than B1, while the B4 was harder than the other groups.
    Conclusion
    The application of the mixture of Lactococcus lactis, Leuconostoc mesenteroides bacteria and baker’s yeast (B2) is suggested to replace the traditional method (B1), on the basis of sensory evaluation and TPA analysis of the results.
    Keywords: Baker's yeast, Barbai Bread, Kluyveromyces Marxianus, Lactococcus lactis, Leuconostoc mesenteroides, Staling}
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