جستجوی مقالات مرتبط با کلیدواژه « maceration » در نشریات گروه « پزشکی »

Nowadays, there is increasing attention to the discovery of new bioactive substances from marine sources. This research aimed to characterize the phytochemical composition as well as antioxidant and antimicrobial activities of Tunisian Ruppia cirrhosa extracts (RCEs) using two different extraction methods.

RCEs were obtained by two different extraction

maceration and successive extraction. The determination of polyphenolic contents and antioxidant activity was made by calorimetric assay, and the effect of RCE was observed against pathogenic bacteria and fungi using the solid diffusion method.

The successive extraction of R. cirrhosa extract relatively showed higher total phenol (38.1 mg GAE/g) and condensed tannin (18.07 mg CE/g) contents than the maceration extraction (35.43 mg EAG/g and 12.99 mg CE/g, respectively). However, the total flavonoid amount of RCE was higher in the maceration extraction (33.09 mg CE/g) than in the successive extraction (21.27 mg CE/g). The total antioxidant capacity of RCE indicated a decrease in this activity after fractionation. Indeed, the activity of RCE decreased from 47.8 to 37.83 mg GAE/g, and RCE obtained by the two extraction methods showed moderate antioxidant activity using reducing power (IC50=380-490 µg/mL) and β-carotene bleaching (IC50=110-310 μg/mL) assays. Furthermore, RCEs obtained by maceration had the greatest antibacterial activity against all tested strains (IZ=3.33-9.33 mm) except Salmonella typhimurium (IZ=2 mm), Enterococcus faecalis (IZ=6 mm), and Streptococcus aureus (3.67 mm) as compared to those obtained by successive extraction. The strains of Candida had a sensitivity for R. cirrhosa extracts obtained by maceration. Indeed, R. cirrhosa extracts obtained by successive extraction had higher inhibitory activity against Candida krusei deduced through an inhibition diameter of 6 mm.

It can be concluded that R. cirrhosa extract is rich in bioactive molecules, and it has an extremely promising biological potential.

Ferulago angulata is a plant known for its phenolic compounds with antioxidant activity. This study aimed to evaluate the flavonoid content antioxidant, and antibacterial properties of F. angulata extract obtained through maceration and ultrasound-assisted extraction methods. Optimal extraction conditions for flavonoid content were determined. The maceration method yielded the highest flavonoid content (276.45 µg QE/ml) with 4 hours of extraction, 250 rpm stirring speed, and 100% ethanol solvent. The ultrasound-assisted method achieved maximum flavonoid content (375.12 µg QE/ml) with 15 minutes of extraction, 140 rpm stirring speed, and 100% ethanol solvent. The maceration extraction method showed the largest inhibition zone diameter, particularly against Staphylococcus aureus, a gram-positive bacterium, indicating strong antibacterial activity. The smallest inhibition zone diameter was observed against Escherichia coli, a gram-negative bacterium. The extraction method significantly influenced the antibacterial properties of the extracts. These findings contribute to understanding the potential applications of F. angulata as a natural source of antioxidants and antibacterial agents.

The species of Arctium lappa L. roots and Polygonum aviculare L. grass have various therapeutic properties in traditional medicine in East Asian countries. The aim of this study was to investigate the effect of three methods of extracting extracts by ultrasonic bath, maceration, and soxhlet to determine phenolic compounds of Arctium lappa L. roots and Polygonum aviculare L. grass using water and 50% ethanol and the evaluation of extraction efficiency of their extract. The results showed that in the ultrasonic bath extraction method, ethanolic extract of Arctium lappa L. root had the highest content of phenolic compounds (976 mg equivalent to Gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to Polygonum aviculare L. grass when using both solvents (14%). In the maceration extraction method, the ethanolic extract of Arctium lappa L. root showed the highest content of phenolic compounds (976 mg equivalent to Gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to the Polygonum aviculare L. grass extracted with water (12%). Also, in the Soxhlet extraction method, ethanolic extract of Arctium lappa L. root showed the highest content of phenolic compounds (578 mg equivalent of gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to the Polygonum aviculare L. grass extracted with ethanol (18/16%). The content of extraction of bioactive compounds based on the type of solvent, the method, and the type of plant used were significantly different from each other and 50% ethanol solvent was the best solvent for extracting the desired compounds. Arctium lappa L. roots also had the highest phenolic composition and extraction by maceration, soxhlet, and ultrasonic bath methods had the most significant effect on the extraction of phenolic compounds, respectively.

Variety of extraction methods coupled with definite solvents could increase the removal rate ofmajor constituents from plants. This research has been conducted to evaluate the effect of extraction methods on the main group of compounds, cytotoxicity, anti acetylcholinesterase (AChE) and antioxidant activity of Trachyspermum ammi fruits.

To compare the quality of extracts earned from maceration and reflux techniques, the amounts of total phenolics, flavonoids and antioxidant property of T. ammi’s fruits extracts were determined; moreover, the cytotoxic activity against Human acute lymphoblastic leukemia (ALL) cell lines (NALM-6) was conducted using MTT assay. Anti-acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activity of both extracts were also examined by Ellman’s method.

The extraction yield of the plant was significantly higher for maceration compared to reflux extraction. Also, both antioxidant activity and total flavonoid contents (IC50=132.95 µg/mL and 140.15 mg catechin/g dry extract, respectively) showed higher amounts considerably in the maceration extraction. In reverse, the content of phenolic compounds (147.28 mg gallic acid/g dry extract and 16.6 mg thymol/g dry extract) was elevated in the refluxed extract. The result exerted moderate inhibition on butyrylcholinesterase activity (IC50= 394.161 µg/mL) and cytotoxicity (IC50 =166.92±1.76 μg/mL for NALM-6 cell line) of the extract using maceration.

The maceration method could provide additional amounts of major constituents and greater biological properties compared to the reflux technique.

Cancer is a term for a large group of different diseases, all involving uncontrolled cell growth. Many of Euphorbiaceae plants have been traditionally used for the treatment of ulcers, tumors, warts, and other diseases. In addition, in the last decade, there are studies showing cytotoxic effects of different species of Euphorbia on tumor cell lines. In this study, we attempted to determine if Euphorbia turcomanica possess any cytotoxic activity.

Solvents extracted the plant powder with various polarities by a maceration method, and qualitative phytochemical analyzes were carried out on them to identify the constituents. On the other hand, the possible cytotoxicity of different extracts on Hela and HT‑29 tumor cells was measured by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay and 50% reduction in cell survival was considered as a cytotoxic effect. Analyze of variance followed by Student‑Newman‑Keuls test was used to see the differences among the groups.

Phytochemical analysis of E. turcomanica showed the presence of flavonoid, alkaloid, anthraquinone and tannin in plant aerial parts. Methanol‑water, acetone, dichloromethane, methanol, and heptane extracts of E. turcomanica significantly reduced viability of Hela cells (P < 0.05) with inhibitory concentration 50% (IC50) of 50, 90, 230, 420, and 450 µg/ml, respectively. While methanol‑water, dichloromethane, methanol, ethyl acetate, and heptane extracts were cytotoxic with IC50 of 43, 115, 125, 250, and 390 µg/ml, respectively (P < 0.05), on HT‑29 cells.

It can be concluded that E. turcomanica is a good candidate for further study toward cytotoxic agents.