جستجوی مقالات مرتبط با کلیدواژه « microbiological profile » در نشریات گروه « پزشکی »

A new type of corona virus has caused Corona virus disease-19 and, subsequently, a global pandemic. All individuals are prone to the disease, so drastic measures were taken to prevent its spread. This study aimed to evaluate the impact of COVID-19 on the progression of the antimicrobial resistance rate by comparing two periods: before and during COVID-19.

We used a cross-sectional design to investigate the Antimicrobial Resistance (AMR) rate before (03/2019 to 03/2020) and during COVID-19 (03/2020 to 03/2021) in a University Hospital in Marrakech. The data were analyzed using SPSS Version 25.0.

Among the 7106 specimens, there was a significant increase in the multidrug-resistant bacterial from 27.38% to 35.87% during COVID-19 (p<0.001), particularly in blood culture, cerebrospinal fluid, catheter, and pus. However, there was a non-significant change in puncture fluid, expectoration, protected distal sampling, joint fluid, stool culture, and genital sampling. A decrease in Multidrug-resistant bacteria (MDRB) was observed only in cytobacteriological urine tests (p<0.05). According to species, there was an increase in extended-spectrum beta-lactamase-producing Enterobacteriaceae, carbapen- em-resistant Enterobacteriaceae, and methicillin-resistant Staphylococcus aureus.

In our study, it is particularly noticeable that the MDRB has increased. These results highlight the importance that the pandemic has not been able to slow the progression.

Tyramine and Histamine, are two major biogenic amines that could be formed in different types of cheese.  Bunium persicum essential oil (BPEO) in different concentrations (0.05, 0.1, 0.2 and 0.4% (v/v)) was added to milk. The amount of biogenic amines (tyramine and histamine) was specified by reverse phase HPLC (RP-HPLC) after extraction from the cheese. Different microbiological analyses (mesophilic lactobacilli, aerobic mesophilic bacteria, Enterobacteriaceae, lactococci, enterococci, and yeasts) were performed during the ripening cheese.  The amounts of biogenic amines were lower in cheese samples containing 0.1, 0.2, and 0.4% BPEO in comparison with control samples. Tyramine quantity increased from 8.90 mg/kg on started day up to 172.40 mg/kg on last day of ageing in the control group also the mean concentration of histamine was 6.31 mg/kg then reached to 41.16 mg/kg in the control samples  at the end of study period. The most microbiological decrease was detected in yeasts, and the minimum microbiological decrease was observed in Enterobacteriaceae population. All the cheese samples were acceptable to the panelists. The increase of BPEO concentrations in cheese samples caused a further reduction in biogenic amines quantity and microbial population. Cheddar cheese sample with 0.2% BPEO demonstrated the highest acceptability and quality among all the cheese samples.  The results of this study revealed Bunium persicum EO could be utilized as a reducing agent of biogenic amines, and a natural antimicrobial and flavoring agent in Cheddar cheese, for improving consumers' health.

We aimed to elucidate the pathogenic bacterial and fungal profiles of specimens obtained from suspected ocular infections at Farabi Eye Tertiary Referral Hospital, Tehran, Iran.

In this cross-sectional study, we collected data from ocular specimens taken during the seven-year period of 2011 to 2018, and the results were then retrospectively analyzed. Samples had been obtained from patients who were investigated for ocular infections.

Overall, 16,656 ocular samples were evaluated. The mean patient age was 48.31 ± 26.62 years. Most patients were men (60.33%), and men in the 7th decade of life were the largest represented group. The seasonal distributions of specimen collection sites followed the overall distribution of collection sites by year. Specimens obtained from the cornea were the most common (49.24%), also representing the largest number of specimens in all seasons. The most commonly isolated fungal microorganisms were Fusarium spp., followed by Aspergillus spp. and Candida albicans. Of the 6,556 specimens with positive bacterial cultures, 59% produced gram-positive bacteria, while the remainder produced gram-negative pathogens. The most commonly isolated bacteria were Pseudomonas aeruginosa (17.77%), Staphylococcus epidermidis (13.80%), Streptococcus pneumoniae (13.27%), S. viridans (12.23%), and S. aureus (11.18%).

Most submitted specimens were obtained from the cornea. The most commonly isolated fungal microorganisms were Fusarium spp., followed by Aspergillus spp. and C. albicans. The most commonly isolated bacteria were P. aeruginosa, followed by S. epidermidis and S. pneumoniae.