جستجوی مقالات مرتبط با کلیدواژه "microdilution" در نشریات گروه "پزشکی"

Different essential oils (EOs) with antibacterial activities are promising natural sources for providing novel disinfectant agents for hospital surfaces.

The component and antibacterial effects of six EOs, including Cuminum cyminum (CCEO), Artemisia sieberi (ASEO), Laurus nobilis (LNEO), Ferula gummosa (FGEO), Lippia citriodora (LCEO), and Cymbopogon citratus (CIEO) were assessed by GC-MS and 96-well micro-plates (IC50), against Staphylococcus aureus and Pseudomonas aeruginosa. Then, the antibacterial effects of FGEO, the most effective EO, were evaluated on the trolley surface in a hospital for 1, 3, 5 and 10 min intervals.

While CCEO, ASEO, and FGEO have the highest effects on the growth of S. aureus, CIEO and LNEO showed the best outcomes on P. aeruginosa. In addition, our finding showed that FGEO relatively decreases the growth of S. aureus and P. aeruginosa on the trolley surface (P<0.05).

The studied EOs could be novel encouraging agents to develop further green antimicrobial agents against different infections. In addition, FGEO, one of the best disinfectant agents in the present study, showed relatively strong antibacterial effects on the surface of the trolley.

Using disinfectants that inactivate microorganisms in various industries, from the pharmaceutical to the food service industry, has been increasing over the last few years. Antimicrobial disinfectants are used as primary treatment options against pathogens on surfaces in healthcare facilities to help prevent healthcare-associated infections. The aim of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the disinfectants used and study the corrosion of surfaces in contact with these disinfectants.

In this study, MICs and MBCs of the disinfectants against B. cereus, S. cerevisiae, P. aeruginosa, and S. epidermidis were measured by the microdilution method. Ethanol, percidine, benzalkonium chloride, and deconex 50ff were examined for antimicrobial effects.

The findings showed that S. cerevisiae had the lowest level of disinfectant resistance, whereas B. cereus consistently exhibited higher resistance. Additionally, when compared to other disinfectants at lower concentrations, deconex 50ff was the most potent disinfectant that resulted in microbial growth suppression. Deconex 50 FF had MICs of 0.101, 12.939, 3.235, and 1.617mg/L for S. cerevisiae, B. cereus, P. aeruginosa, and S. epidermidis, respectively. In contrast, MBC of S. cerevisiae, B. cereus, P. aeruginosa, and S. epidermidis were 0.404, 25.879, 12.939, and 6.470 mg/L, respectively. Macroscopic analysis of stainless steel and plastic sandwiches treated with the specified disinfectant concentrations revealed no corrosion.

Considering the importance of these microbial strains in healthcare-associated infections, using these effective disinfectants is recommended in the pharmaceutical industry.

Essential oils (EOs) with different biological activities, such as antibacterial properties, are a valuable resource for developing new drugs.

Ingredients of six medicinally important EOs, including Artemisia dracunculus, Anethum graveolens, Citrus limon, Citrus sinensis, Cinnamomum zeylanicum and Zingiber officinale, were identified using GC-MS analysis. Moreover, their five major compounds were also listed. Furthermore, the half-maximal inhibitory concentration (IC50) against four important human bacteria was also investigated using the 96-well plate microdilution.

C. sinensis EO with IC50 of 1.0 and 4.7 mg.mL-1 have the most effect on the growth of S. aureus and P. aeruginosa. Moreover, EOs of Cinnamomum zeylanicum (IC50: 1.0 mg. mL-1) and Artemisia dracunculus (IC50: 1.3 mg.mL-1) significantly showed better inhibitory effect on E. coli and K. pneumoniae.

These EOs could be used for developing inexpensive, potent, and green antibacterial agents.

Dermatophytes are a group of fungi specialized in invading humans and other vertebrate keratinized tissues. These fungi cause a variety of skin, nail, and hair disorders, called dermatophytosis (tinea). In some cases, drug resistance to antifungals necessitates special treatment. Among the antifungal agents, sertaconazole (i.e., a third-generation imidazole) has a broad-spectrum against dermatophyte species. Regarding this, the present study was conducted to investigate the antifungal susceptibility of dermatophytes obtained from patients with dermatophytosis in Mashhad located in northeastern Iran.

A total of 75 clinical dermatophyte isolates, including Trichophyton mentagrophytes (n=21), T. interdigital (n=18), T. tonsurans (n=16), Epidermophyton floccosum (n=11), Microsporum canis (n=5), Nannizzia fulvum (n=2), T. benhamiae (n=1), and T. verrucosum (n=1), were evaluated against five antifungal agents of sertaconazole, itraconazole, clotrimazole, terbinafine, and griseofulvin based on the CLSI M38-A2 guideline.

According to the results, the minimum inhibitory concentration (MIC) ranges of sertaconazole, terbinafine, griseofulvin, itraconazole, and clotrimazole were estimated at 0.125-16, 0.002-1, 0.5-4, 0.031-4, and 0.016-4 μg/ml, respectively, for dermatophyte species. In addition, the geometric mean (GM) values of the MIC of sertaconazole, terbinafine, griseofulvin, itraconazole, and clotrimazole were obtained as 3.39, 1, 1.44, 1.52, and 1.93, respectively.

Among the tested antifungals, terbinafine and griseofulvin were the most effective agents against dermatophyte isolates. However, sertaconazole, a third-generation imidazole, did not show any significant effect. Furthermore, M. canis and E. floccosum showed the best response to the antifungal agents.