جستجوی مقالات مرتبط با کلیدواژه "mpo" در نشریات گروه "پزشکی"

Tumor necrosis factor-alpha (TNF-α) may stimulate airway hyperresponsiveness in asthma, which is also affected by neutrophils activity. The latter can be determined indirectly by evaluating myeloperoxidase (MPO) activity. The insufficient studies that investigated the combined association of
serum TNF-α and MPO with asthma was objective of this study.

A case-control study included 110-asthmatics besides 92-controls. All participants underwent venous sampling for TNF-α and MPO  immunoassays. A percentage of predicted ''forced expiratory volume in one second (FEV1%)'', and the ''peak expiratory flow rate (PEF/L)'' of all participants were verified. The statistical analyses had done using SPSS V-25. The accuracy, specificity, sensitivity, and significance of both biomarkers to distinguish asthma examined ''under the ROC-curves''.

High TNF-α levels observed among the controls(p-0.006), opposing the higher MPO levels among the patients(p-0.00). There were nonsignificant variations of two biomarkers between the treatment groups and nonsignificant correlations of MPO with FEV1 and PEF. There was a significant correlation of MPO with the TNF-α levels of all participants. The TNF-α showed lower sensitivity, specificity, and accuracy to diagnose asthma. There were no MPO differences according to asthma levels. The TNF-α was higher among the severe asthmatics significantly.

TNF-α may be a contributory particle for neutrophilic inflammation of severe asthma. MPO levels were significantly higher among asthmatics, whereas TNF-α levels were lower. TNF-α levels were higher among those with severe compared to mild/moderate asthma. The MPO level has a significant
predictive capacity compared to TNF-α for distinguishing asthma from healthy subjects.

The contribution of neutrophils is still indistinct in the inflammatory response of bronchial asthma (BAs). Myeloperoxidase (MPO) is an  enzyme released from the primary azurophilic granules of the neutrophils. The study aimed to evaluate the levels of serum MPO as a biomarker for the assessment of the level of asthma control. 

The study participants included 94 asthmatic patients and 86 healthy controls. The identification of asthma severity had assessed using the ''Global Initiative for Asthma guidelines''. Asthmatic adults had divided into three groups: Good (n= 22), partial (n= 30), and poor control (n=44). Also, patients have been divided again into two groups (treated and untreated) for BAs.

The predicted FEV1% and the peak expiratory flow (PEF/L) of all participants had verified by spirometry. The mean patients’ age was 31.9±15.1 year, with a predominance of females. The mean asthma duration was 10.5±8.6 years. Mean spirometric parameters (FEV1 and PEF) were significantly lower among the patients (0.00). Significant higher MPO levels had observed among BAs patients (p-0.00). The MPO levels have not differed significantly with asthma levels and had significant differences with the history of treatment. There was a nonsignificant negative correlation between the mean MPO levels and the spirometry variables among the patients. ROC curves revealed a sensitivity, specificity, accuracy for MPO (80.9%, 72.1%, and 84.3%), respectively to predict asthmatic severity.

There were significantly higher MPO levels compared to healthy controls. Levels of serum MPO had a non-significant positive correlation with levels of asthma control, but a negative non-significant correlation with spirometric results.

Traditionally, Ocimum basilicum has been used for its anti-inflammatory activity. Here, the effect of O. basilicum ethanol extract was evaluated on carrageenan induced paw edema in male Wistar rats. O. basilicum ethanol extract was achieved through maceration procedure. Next, the paw inflammation was induced with 1% carrageenan, subcutaneous, to all groups after 1h of the intrapritoneal injection of O. basillicum extracts (0, 2.5, 5 and 10mg/kg; respectively). The paw thickness was measured at hourly intervals for 4h. Finally, histological examination and Myeloperoxidase (MPO) activity was assessed in the inflamed paw at the 4thh. Moreover, total phenolic and flavonoid content of the extract was determined. Besides, different in vitro antioxidant activities were detected by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, nitric oxide radical inhibition and reducing power methods. The extract diminished paw inflammation as indexed by reduction paw thickness (p<0.001) as well as MPO activity (p<0.001), which was associated with a marked decrease in tissue edema and leukocyte infiltration. In addition, values for the total phenolic and flavonoids were calculated as 266 mg gallic acid equivalent and 65 mg quercetin equivalent per 100g of O. basilicum fresh plant material. Furthermore, the RC50 values for DPPH and nitric oxide antioxidant activity of the extract was determined as 118µg/mL and 929.7µg/ml. Ethanol extract of O. basilicum significantly decreased the inflammatory reaction induced by carrageenan which could be related to its antioxidant effects.