جستجوی مقالات مرتبط با کلیدواژه "nr1" در نشریات گروه "پزشکی"

The decrease of GLT-1 is associated with the activation and increased expression of the glutamate receptor subunit, NR1, which causes neuropathic pain in multiple sclerosis (MS) by increasing basal cell activity. This study aimed to compare the effect of four weeks of swimming and living in an enriched environment on GLT-1, NR1, and c-FOS, a marker of neuronal activity, in the dorsal horn of the spinal cord in experimental autoimmune encephalomyelitis (EAE), an animal model used to study of MS.

forty female C57BL6 mice were randomly divided into four groups: EAE, control, swimming exercise, and enriched environment. The healthy control group received a saline injection, other groups were induced with myelin oligodendrocyte glycoprotein (MOG35-55). After the induction of EAE, the swimming group performed swimming for 30 minutes, 5 days a week for four weeks, and the environmental enrichment group lived in an enriched environment for four weeks. On day 30 post-induction, the mice were sacrificed, and the spinal cord tissue was removed. GLT-1, NR1, and c-FOS protein levels were measured by immunohistochemistry.

The findings showed that living in an enriched environment and swimming exercise both significantly increased GLT-1 levels and significantly decreased NR1 and c-FOS levels compared to the EAE group. Although, living in an enriched environment compared to swimming exercise, showed that the first was a more effective method.

Exercise, in addition to reducing disease severity, can reduce pain sensitivity in the EAE mice and increase their quality of life by increasing GLT-1, and decreasing NR1 and c-FOS in the spinal dorsal horn.

Sleep deprivation has negative effects on consciousness and performance. N-methyl-D-aspartate receptors are a subset of glutamate receptors consisting of subunits NR1 and NR2. In the present study, the effect of a traditional Chinese medicine supplement called NeuroAid on the levels of NR1 and NR2 proteins in the striatum and prefrontal cortex of rat under total sleep deprivation (TSD) was evaluated.

ats were randomly divided into four groups of control (n=5), sham (n=5), TSD (n=5), TSD + supplementation (n=5) and sham + supplemention (n=5). After 24 hours of testing, the prefrontal cortex and striatum of the rats were removed and the levels of NR1 and NR2 proteins were assessed by Western blotting. Differences between protein levels were analyzed using one-way analysis of variance (ANOVA) and t-test.

TSD reduced the expression of both NR1 and NR2 proteins in the striatum and prefrontal cortex of rats. There was no significant difference in the expression of any proteins in the two regions of sham group compared to the control rats. Two intraperitoneal injections of 0.8 mg/kg NeuroAid significantly increased the expression of both proteins in both regions compared to the TSD group. In the sham + supplemention group, the expression of both proteins in the two regions increased significantly compared to the sham group.

The use of NeuroAid supplement can increase the levels of NR1 and NR2 proteins in rats with TSD.