جستجوی مقالات مرتبط با کلیدواژه « opg » در نشریات گروه « پزشکی »
-
Introduction
The aim of this study was to investigate the histomorphometric effects of bone marrow-derived mesenchymal stem cells (MSCs) transfected with Adeno Associated Virus (AAV) and containing bone morphogenic protein 7 (Bmp7) or osteoprotegerin (OPG) on bone formation after injection into inter-premaxillary suture in rats with Bmp7 or OPG alone.
Materials and MethodsIn 4 groups (each group n:9), different chemical solutions, namely AAV-Bmp7, AAV-OPG and AAV-Bmp7-OPG and AAV-EGF (control group) were injected into the interpremaxillary suture of rats. Bone volumes (BV), soft tissue volume (STV) and total bone volumes (TBV) of 10μm serial selection with hemotoxylin and eosin staining were calculated according to the Cavalieri principle. Each point in the region of interest was 1000μm3.
ResultsThe BV for AAV-Bmp7, AAV-OPG, AAV-Bmp7-OPG and AAV-EGF were 46.98±1.50 mm3, 49.40±4.72mm3, 42.58±2.89mm3 and 38.82±0.76mm3, respectively. The STV was 11.53±0.99, 13.31±1.88, 8.00±4.43 and 9.57±1.90mm3 for Bmp7, OPG, AAV-Bmp7-OPG and AAV-EGF, respectively. TBV was 58.34±2.28mm3, 63.83±5.17mm3, 53.74±3.34mm3 and 48.13±1.54mm3 for AAV-Bmp7, AAV-OPG, AAV-Bmp7-OPG and AAV-EGF, respectively. The comparison between BV, STV, TBV for AAV-OPG showed a statistically significant difference (p=0.001) compared to AAV-Bmp7 or AAV-Bmp7-OPG and AAV-EGF.
ConclusionDuring tooth movement and bone remodeling, the ratio of soft and bone tissues is maintained by OPG. Although Bmp7 is not as effective as OPG in bone remodeling, both can reduce the retention time and the risk of recurrence.
Keywords: Histomorphometry, Bmp 7, OPG, Maxillary Expansion, Orthodontics, Rats} -
مقدمهنهفتگی دندان کانین یکی از اختلالات تکاملی بوده که مشکل بسیار شایعی در تمام جوامع دنیا از جمله نوجوانان ایرانی است. نهفتگی کانین می تواند مشکلاتی برای دندان های مجاور و نیز فکین ایجاد کند. لذا تشخیص زودهنگام این مساله اهمیت دارد. هدف از انجام این مطالعه، تعیین فراوانی و بررسی تمایل محوری کانین های نهفته بر اساس رادیوگرافی پانورامیک در مراجعه کنندگان به دانشکده دندانپزشکی مشهد بود.مواد و روش هادر این مطالعه توصیفی، مقطعی، تعداد 4058 رادیوگرافی پانورامیک مورد بررسی قرار گرفت. شیوع نهفتگی دندان های کانین بر اساس متغیرهای سن، جنس، همراهی با سایر دندان های نهفته و تمایل محوری آنها بر اساس سیستم طبقه بندی Yamamoto انجام گرفت. همچنین ارتباط ناحیه نهفتگی دندان کانین و نهفتگی دندان های همراه با جنس بررسی شد. داده های مطالعه با استفاده از آزمون Chi-Square مورد تجزیه و تحلیل قرار گرفت. در ضمن سطح معنی داری 05/0 لحاظ شد.یافته هااز 4058 مورد رادیوگرافی پانورامیک ، 38 مورد (94/0%) دارای نهفتگی دندان کانین ماگزیلا بودند. فراوانی کانین های نهفته در زنان (9/57%) بیشتر از مردان (1/42%) و در بازه سنی 20-16 سال مشاهده شد. از نظر همراهی با سایر دندان های نهفته، بیشترین فراوانی مربوط به دندان مولر سوم مندیبل بود (46/38%). همچنین نهفتگی نوع II بیشترین فراوانی را داشت (3/58%). از نظر آماری ارتباط معنی داری بین ناحیه نهفتگی دندان کانین و همراهی نهفتگی سایر دندان ها با جنس وجود نداشت (05/0P>) .نتیجه گیریبا توجه به شیوع بالای نهفتگی دندان کانین ماگزیلا در جمعیت مورد مطالعه، احتمال نهفتگی این دندان ها به خصوص طی معاینات روتین دوره نوجوانی و بالاتر همواره در تصاویر رادیوگرافی بایستی مدنظر قرار بگیرد. در کلیشه پانورامیک موقعیت اکثر دندان های کانین نهفته نسبت به پلن اکلوزال، همراه با تمایل مزیالی بود.کلید واژگان: کانین, شیوع, نهفتگی, رادیوگرافی پانورامیک}IntroductionImpaction of maxillary canines is one of the common developmental disorders in all societies worldwide, including Iranian adolescents. It can bring about serious problems for adjacent teeth and the jaw. Therefore, early detection of this issue is of utmost importance. The present study aimed to determine the frequency and axial inclination of impacted canines based on OPG radiography in patients referred to Mashhad Dental School.Materials and MethodsIn this descriptive, cross-sectional, and retrospective study which assessed 4,058 OPG radiographs in Mashhad Dental School, the prevalence of impacted canines was determined based on age, gender, region, the existence of other impacted teeth, and axial inclination based on Yamamoto’s classification system. Moreover, the relationship of gender with impacted tooth region and existence of other impacted teeth. A p-value of 0.05 was considered statistically significant.ResultsOut of 4, 058 OPG radiographs, 38 (0.94%) cases had maxillary canine impaction. The abundance of impacted canines was higher in women (57.9%) than men (42.1%) and in the age range of 16-20 years. Regarding association with other impacted teeth, the highest frequency was related to the mandibular third molar (38.46%). Out of 38 cases of impacted canines, the most frequent type was type II impaction (58.3%). The results of the Chi-Square test demonstrated that gender was not significantly correlated with the impacted tooth region and the existence of other impacted teeth.ConclusionAs evidenced by the obtained results, the prevalence of maxillary canine impaction in the study population was high, and therefore, the impaction of this tooth should always be considered in radiographic images, especially in routine examination duration adolescents and adults.Keywords: canine, Prevalence, impaction, OPG}
-
BackgroundIntroduction
Oxytocin (OT) has been proposed to assist in the regulation of bone remodeling and to exert an antiosteoporotic effect. We evaluated the possible protective effect of OT against bone degeneration in ovariectomized (OVX) rats.
MethodsThe study was performed on three groups of adult female rats; group I was subjected to sham operation, group II was subjected to ovariectomy, and group III was subjected to ovariectomy and intraperitoneal injection with OT for eight successive weeks. At the end of the study, bone mass density (BMD) was measured; then the rats were euthanized and their blood and bone tissues were examined.
ResultsThe group II rats had significantly less BMD and greater serum bone-specific alkaline phosphatase (bALP), osteocalcin (OC), and tartrate-resistant acid phosphatase (TRAP) levels than the group I rats. Furthermore, group II rats had fewer osteocytes and osteoblasts, and less OPG/RANKL mRNA expression than group I rats. The groups I and III and rats showed no significant differences in BMD, bALP, OC, TRAP, OPG/RANKL mRNA expression, or osteocyte and osteoblast numbers.
ConclusionsOxytocin may have an antiosteoporotic effect in OVX rats.
Keywords: Osteoporosis, OPG, Oxytocin, Ovariectomy, RANKLKL} -
Objective(s)To investigate the effect of cocoa on orthodontic tooth movement (OTM) rate, osteoprotegerin (OPG), and receptor activator of nuclear factor κ β ligand (RANKL) levels after OTM.Materials and MethodsA total of 24 Sprague-Dawley rats were included in the study. They were equally divided into two groups: cocoa and control. The upper incisors of all rats were subjected to 35 cN orthodontic force and moved distally with a stainless steel 3-spin coil spring. During OTM, the cocoa group was given 4.8 g of unsweetened cocoa once a day. At 4 subsequent time points (0, 1, 7, and 14 days), the OTM rate was determined by measuring the distance between the mesial tips using a digital caliper, while OPG and RANKL levels were examined based on their gingival crevicular fluid through specific enzyme-linked immunosorbent assay (ELISA). Data gathered were analyzed through independent t-test (P<0.05).ResultsThe OTM rate of the cocoa group was significantly higher than that of the control group on days 1, 7, and 14 (P<0.05). ELISA analysis revealed that the OPG level was significantly lower on day 14. Furthermore, the RANKL level was significantly higher on days 0, 1, and 7 for the cocoa group compared with the control group (P<0.05).ConclusionThese results indicate that cocoa has the potential effect to modulate the OTM rate by inducing osteoclastogenesis, which suppresses the OPG level and stimulates the RANKL level, in rats.Keywords: Caffeine, Cocoa, OPG, Orthodontic tooth- movement, RANKL}
-
Rheumatoid arthritis (RA) is one of most important collagen vascular diseases. It has an unknown origin. The aim of this study was to evaluate circulating levels of osteoprotegerin (OPG), total soluble receptor activator of nuclear factor-Kappa B ligand (total sRANKL), and RANKL/OPG ratio in patients with RA. Forty-five females with RA, who fulfilled the American college of rheumatology (ACR) criteria for RA were included in this cross-sectional study. The overall disease activity was evaluated by the disease activity score based on 28 joint counts (DAS-28). The OPG and sRANKL were measured by the enzyme-linked immunosorbent assays (ELISA). The levels of C-reactive protein (CRP) were measured by ELISA.We used Pearsons correlation for our comparisons. There was no statistically significant difference between the levels of CRP, OPG, sRANKL and RANKL/OPG ratio in terms of DAS-28 grades in our patients. No significant correlation was found between the serum levels of OPG and DAS-28 (P= 0.525), duration of the disease (P= 0.884), Z-score of the femur (P= 0.546) and Z-score of the spine (P= 0.492), T-score of the femur (P= 0.137) and T-score of the spine (P= 0.821) in the patient group. No significant correlations were found between sRANKL levels with DAS-28 (0.919), Z-score of the femur (P= 0.971), Z-score of the spine (P= 0.832) and T-score of the femur (P = 0170) in the studied groups. Our study showed that there was no significant correlation between CRP, OPG, sRANKL and RANKL/OPG ratio in DAS-28 grading of our patients. For this reason they will not be used for evaluating disease activity. However, there was a significant difference between case and control groups except for sRANKL (pg/mL).Keywords: ACR criteria, OPG, rheumatoid arthritis, sRANKL}
-
Background And ObjectiveOsteoporosis is one of the complications of diabetes. The aim of this study was to determine the impact of resistance training along with Portulaca oleracea supplementation on OPG and NFκB levels (bone markers) in women with type II diabetes.MethodsOverall, 28 women with type II diabetes (44 to 60 years old) were randomly and equally assigned into four groups (supplement, training, training-supplement and control). An eight-week resistance training program (three one-hour sessions per week with one repetition maximum) was performed using three types of bands with different resistances, at 40 50% intensity and 50-70% maximum heart rate. P. oleracea seeds were supplemented daily (7.5 grams mixed in yogurt) for eight weeks. The data was analyzed using descriptive statistics, paired t-test and ANOVA.ResultsAfter 8 weeks, OPG and NFκB levels in the three groups of supplement, training and training-supplement increased and decreased, respectively. The highest change in both variables was observed in the training-supplement group. Moreover, the level of these two variables in the training-supplement group had significant difference with the controls and other groups (P=0.000).ConclusionAs a non-pharmacological therapeutic approach, the regular resistance training and P. oleracea supplementation can increase bone formation markers and reduce bone resorption in women with type II diabetes.Keywords: Type II Diabetes, Resistance Training, Portulaca Oleracea, OPG, NFκB}
-
Background
The role of host response in periodontitis pathogenesis is confirmed, and it is well established that immune response plays a major role in the alveolar bone destruction. In the investigation of these responses, the role of receptor activator of the nuclear factor‑kB ligand (RANKL)‑osteoprotegerin (OPG) system is the most promising. Smoking can affect the RANKL‑OPG system in a manner that will further enhance bone loss in periodontitis. The aim of this study is to assess the serum, saliva, and gingival crevicular fluid (GCF) concentration of RANKL and OPG in smoker versus nonsmoker untreated chronic periodontitis (CP) patients.
Materials and MethodsThirty‑nine subjects were included in the present cross‑sectional study: 29 systemically healthy CP male patients(15 smokers, 14 nonsmokers) and 10 systemically and periodontally healthy nonsmoker male subjects. Serum, GCF, and whole saliva samples were obtained from the subjects. The enzyme‑linked immunosorbent assay (ELISA) kits were used for assaying the concentrations of RANKL and OPG in the samples. The one‑way analysis of variance (ANOVA) test and the least significant difference (LSD) post hoc test were utilized to compare differences between the groups.
ResultsRANKL and OPG concentrations in saliva, serum, and GCF did not show any significant difference among all groups (P > 0.05). Salivary RANKL/OPG ratios were significantly higher in the nonsmoker CP group than in the healthy control group (P > 0.05) but they were not statistically significant among smoker periodontitis patients.
ConclusionsThe salivary RANKL/OPG ratio was higher in nonsmokers with periodontitis in comparison with smoker periodontitis patients.
Keywords: Gingival crevicular fluid, OPG, periodontitis, RANKL, saliva, serum, smoking} -
IntroductionComparison of the relationships and distance between maxillary root tips and the maxillary sinus floor using oral panoramic in the dolichocephalic and brachycephalic compared to mesocephalic individuals.MethodsOral panoramic images from 300 individuals were analyzed and the relationships and distance between the maxillary root tips and the sinus floor was assessed by qualitative and quantitative variables.ResultsThe distance was significantly higher in the brachycephalic groups than that of the mesocephalic, and the mesocephalic group showed longer distance in comparison to dolichocephalic individuals. Qualitative comparison showed that type 1 relationship was the dominant position in the brachycephalic individuals while most of dolichocephalic individuals demonstrated type 2 and 3 relationships of the molar root tips and the maxillary sinus floor.ConclusionHigher distances between the molar root tips and the maxillary sinus floor could be expected in the brachycephalic than mesocephalic and dolichocephalic individuals.Keywords: Maxillary sinus, Molar teeth, OPG, Maxilla}
-
A Radiographic and Clinical Survey of Dental Anomalies in Patients Referring to Shiraz Dental SchoolStatement of Problem: Many epidemiological surveys have been conducted in different parts of the world to determine the prevalence of various types of dental anomalies. There are regional and ethno-racial variations in the prevalence of dental anomalies.PurposeTo assess the prevalence of dental anomalies in a group of Iranian dental patients in Shiraz dental school, Iran.Materials And Method414 dental patients referring to dental school and aged between 15-60 years old (257 female and 157 male) were examined clinically and radiographicaly for the presence of dental anomalies, using orthopanthomography. Data were collected and analyzed by Chi-square and Fisher exact tests.ResultsFluorosis was the most prevalent anomaly (7.72%) followed by missing of the wisdom teeth (7%), impaction of teeth (4.34%), microdontia (2.89%), missing of the mandibular second premolars (2.65%), supernumerary teeth (2.4%), missing of the maxillary lateral incisors (1.6%), dilaceration (1.44%), invagination (1.44%), and taurodontism (0.96%).ConclusionIn the comparison of these results with those of other studies, it was indicated that these anomalies occur at different frequencies among various coun-tries and communities in the world. Recognizing these anomalies will facilitate the endodontic, prosthodontic, periodontic and surgical management of such teeth.Keywords: Dental anomalies, Prevalence, OPG}
- نتایج بر اساس تاریخ انتشار مرتب شده‌اند.
- کلیدواژه مورد نظر شما تنها در فیلد کلیدواژگان مقالات جستجو شده‌است. به منظور حذف نتایج غیر مرتبط، جستجو تنها در مقالات مجلاتی انجام شده که با مجله ماخذ هم موضوع هستند.
- در صورتی که می‌خواهید جستجو را در همه موضوعات و با شرایط دیگر تکرار کنید به صفحه جستجوی پیشرفته مجلات مراجعه کنید.
©2000-2024 magiran.com All Rights Reserved.