جستجوی مقالات مرتبط با کلیدواژه « oscillatoria » در نشریات گروه « پزشکی »

Breast cancer is the most common cause of cancer. The aim of the current study was to evaluate the cytotoxicity, apoptotic effects and Noxa gene expression of Oscillatoria extract against human breast cancer (MCF-7) cell line. The MCF-7 cells were treated with different concentration of extracts ranging 1.56, 3.12, 6.25, 12.5, 25, 50 mg/mL for 24 and 48 hours. The cytotoxicity activity of Oscillatoria extract towards cancer cells were evaluated by using colorimetric MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay. The Noxa gene expression was measured via real time PCR technique. Finally, apoptotic and necrosis study of extract was analyzed using Flow-cytometry method. Cytotoxic results clarified that the desired extracts have a dose-dependent toxicity effect and viability of the cells significantly decreased. Noxa gene expression levels in MCF-7cells were increased to 33.93 ± 1.6 (P<0.001) compared to the control group. In addition, the flow-cytometry data revealed the 43.07 % apoptosis in breast cancer cell line ‏. It seems that Oscillatoria extract has potential uses for treatment of breast cancer and it suggested that further investigations were performed for Oscillatoria pharmaceutical importance.

Breast cancer is one of the most common cancers in women around the world. Problems, such as treatment failure, drug resistance, heavy costs, other problems associated with the treatment of this type of cancer, have attracted the interest of many researchers to cyanobacteria, since these microorganisms have fewer side-effects compared to chemical drugs. In the present research, the effect of Oscillatoria extract was investigated on the viability of MCF7 cell line as well as caspase-9 gene expression.
In this experimental study, MCF7 cell lines, were treated with different concentrations of extract of Oscillatoria cyanobacteria (0.078, 0.15, 0.3, 0.6, 1.2, 2.5, 5, and 10mg/ml) for 24 hours. The effect of the extract on cell viability was assessed using MTT assay. Then, RNA extraction was carried out, and after synthesis of cDNA, real time PCR was performed to assess the expression rate of caspase-9 gene.
With increasing the concentration of the extract, cell viability significantly decreased compared to the control sample. Real time PCR results also showed that the expression of caspase-9 gene increased by 5.59 ± 0.83 (p Oscillatoria extract can destroy cancer MCF-7 cells, which can be due to the increase in caspase-9 gene expression.

Due to the increase of bacterial resistance to common antibiotics, tendency towards using natural compounds with antimicrobial activity was increased. Mean while, many bacterial species show multi or pan-resistant phenotypes. Many of these multidrug resistant (MDR) bacteria can cause life-threatening infections, being a major concern both in the hospital and the community. Therefore, in this study, we investigated the antimicrobial properties of cyanobacteria Oscillatoria sp.
The antimicrobial effect of cyanobacteria of Oscillatoria in concentrations of 50 mg/ml was investigated. The antimicrobial properties of cyanobacteria extract supernatant using in well diffusion agar was investigated. Moreover the minimum inhibitory concentration (MIC) was performed against human pathogenic bacteria such as, S. aureus, B. subtilis, P. aeruginosa and E. coli.
Findings: We observed the highest antimicrobial activity against E. coli bacteria with inhibition zone a diameter of 24 mm and the lowest antibacterial effect against P.aeruginosa with inhibition zone a diameter 14mm. In addition, the minimum inhibitory concentration of Oscillatoria extract against E. coli and Pseudomonas was documented in 3/125 and 12/5 mg/mL respectively.
Our results indicated that the methanolic extract of Oscillatoria had the proper antimicrobial activity against human pathogens and this inhibiotion was dose-dependent.

The discipline of nano-biotechnology is emerging to be a most promising area of research in medicine. Recently, biological methods for the synthesis of metallic nanoparticles have been attained for killing diseases. This study investigated the anti-cancer and antibacterial properties of biosynthesized silver nanoparticles (AgNPs) by Oscillatoria extract.
The biosynthesis of silver nanoparticles was performed by using Oscillatoria extract as a reducing and capping agent. The characterization of biosynthesized nanoparticles was evaluated by X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The antibacterial effect was primarily used by minimum inhibitory concentration (MIC) using broth Macro dilution method. The cytotoxicity effect of silver nanoparticles against cell lines was investigated by using MTT assay. Also, AnnexinV/propidium iodide (PI) kit and flow cytometry technique was performed for determination of apoptotic and necrosis properties of treated nanoparticles.
The biosynthesized silver nanoparticles were exhibited in the average of 30 nm and mostly spherical. Antibacterial activities revealed that the MIC value of these biosynthesized silver nanoparticles against E. coli, A. baumannii, and S. aureus was 25, 50 and 12.5 μg/ml, respectively. The data show that the biosynthesized AgNPs exert a dose-dependent inhibitory effect on viability of cells. Moreover, the flow-cytometry results reveal the 35% apoptosis in breast cancer T47D cells.
According to the result of this work, the biosynthesized silver nanoparticles have showed anticancer and antibacterial activities. It can also be investigated further to use in treatment of cancer and pathogenic bacterial infections.