جستجوی مقالات مرتبط با کلیدواژه "oxidant activity" در نشریات گروه "پزشکی"
-
Objective(s)Biological applications of gold nanoparticles have limitations because of the toxic chemicals used in their synthesis. Curcumin can be used as reducing as well as capping agent in synthesis of GNPs to eliminate the cytotoxicity. Conjugation of curcumin to gold also helps in increasing its solubility and bioavailability.Materials And MethodsHere we report synthesis of gold nanoparticles coated with citrate and curcumin and of two different sizes via chemical routes. UV-Vis absorbance spectroscopy, Dynamic Light Scattering and Transmission Electron Microscopy were applied to study the average particle size, size stability of the samples and zeta potential. Fourier transform infrared, Raman Spectroscopy and Fluorescence Spectroscopy were applied for detection of curcumin on the surface of GNPs. The antioxidant activity was evaluated using DPPH assay and Cytotoxicity was evaluated by MTT assay.ResultsParticles were synthesized of 6 and 16 nm size. The average particle size was found to be 21.7 ± 5.7 by TEM. The zeta potential on the surface of Cur-GNPs was negative and larger than 25 mV which is a sign of their high stability. The stability of these particles (with different coatings but with similar sizes) at different time intervals (up to 3 months) and also in different media like cell culture medium, different buffers, glucose and at different pH conditions have been investigated thoroughly. Appearance of functional groups assigned to curcumin in FTIR and SERS spectra are sign of presence of curcumin in the sample. The quenching of the fluorescence in the presence of GNPs reveals the clear indication of the capping and binding of curcumin with GNPs. Cur-GNP1 (16 nm) were found to exhibit highest antioxidant activity than other gold nanoparticles. Cytotoxicity evaluation using MTT assay on L929 cell line proved curcumin coated gold nanoparticles were non-toxic up to 40 ppm.ConclusionThe results revealed that larger curcumin coated gold nanoparticles were stable and also non-toxic and were found suitable for further in-vitro and in-vivo studies.Keywords: Anti, oxidant activity, Curcumin, Gold nanoparticles, Green synthesis
-
سابقه و هدفامروزه تقاضا برای فراورده های پروبیوتیک غیرلبنی به دلیل پرطرفدار شدن گیاه خواری، عدم تحمل لاکتوز شیر در برخی افراد و میزان بالای کلسترول فراورده های لبنی افزایش یافته است. بنابراین هدف از این مطالعه بررسی اثر نگهداری یخچالی بر قابلیت زیستی پروبیوتیک ها و برخی ویژگی های فیزیکوشیمیایی در آب زغال اخته است.مواد و روش هاآب زغال اخته با pH طبیعی و تعدیل شده با سدیم بی کربنات توسط سه سویه صنعتی پروبیوتیک شامل لاکتوباسیلوس رامنوسوس، ل. پلانتارم و ل. کازئی و دو سویه بومی ل. کازئی تلقیح شد. نمونه ها بلافاصله پس از تلقیح وارد یخچال (°C4) شدند. سپس ویژگی های شیمیایی از جمله pH، اسیدیته، میزان مواد فنلی، آنتوسیانین ها و فعالیت ضد اکسیدانی، ویژگی های میکروبی از جمله شمارش سلول های زنده پروبیوتیک و خواص حسی نمونه ها به مدت 4 هفته با فواصل زمانی یک هفته برای نمونه های با pH تعدیل شده مورد بررسی قرار گرفت. تجزیه و تحلیل داده ها با استفاده از آزمون آنالیز واریانس یک طرفه(One way ANOVA) و Repeated Measure و نرم افزار SPSS انجام شد. سطح معنی داری P<0.05 در نظر گرفته شد.یافته هانتایج حاصل از آنالیز آماری داده ها نشان می دهد که نوع تیمار، زمان نگهداری و اثر متقابل این دو عامل بر تغییرات ویژگی های فیزیکوشیمیایی و قابلیت زیستی پروبیوتیک ها در آب زغال اخته تاثیر معنی داری دارد (0/05>P). نتایج نشان داد که سویه بومی ل. کازئی زیر گونه T4 در مقایسه با سایر پروبیوتیک ها، قابلیت زیستی خود را بعد از 4 هفته نگهداری یخچالی در تعدادlog cfu/ml 67/8 حفظ نمود. همچنین بیشترین تغییرات در ویژگی های فیزیکوشیمیایی، در تیمار های آب زغال اخته حاوی این سویه پروبیوتیک مشاهده شد که نشان دهنده ی فعالیت تخمیری آن طی نگهداری یخچالی است.نتیجه گیرینتایج نشان داد که آب زغال اخته به دلایلی نظیر pH پایین و وجود ترکیبات فنلی بازدارنده بر قابلیت زیستی پروبیوتیک ها به ویژه سویه های صنعتی طی نگهداری یخچالی اثر منفی دارد.
کلید واژگان: پروبیوتیک, زغال اخته, فعالیت ضد اکسیدانی, قابلیت زیستی, مواد فنلیBackground And ObjectivesRecently, an increased demand for nondairy probiotic products comes from the popularity of vegetarianism, lactose intolerance, and high cholesterol content in dairy products. The objective of this study is evaluating the effect of refrigerated study on the viability of probiotics and some physicochemical characteristics in cornelian cherry juice.Materials And MethodsCornelian cherry juice with natural and adjusted pH with sodium bicarbonate was inoculated with three industrial bacteria (L. rhamnosus, L. plantarom and L. casei) and two native probiotic bacteria (L. casei) (108 cfu/ml). Then the samples were placed in the refrigerator (4°C) immediately after inoculation. Next, the changes in chemical characteristics such as pH, acidity, phenolic compounds content, anthocyanin content, antioxidant activity, microbial characteristics (such as enumeration of live probiotic cells), and sensory properties were evaluated during 4 weeks with the time intervals of one week for the samples with adjusted pH. Significant differences between the averages of obtained data were checked by one way ANOVA test. Also differences between the averages of each treatment during the refrigerating time were examined by repeated measure test using SPSS software at the significant level of 0.05.ResultsData analysis showed that treatment type, storage period and interaction effect of these two factors have significant effects on the changes of physicochemical characteristics and probiotic viability (P<0.05). The results revealed that native probiotic strain L. casei spp. T4 compared to other strains maintained its viability at 8.67 log cfu/ml after 4 weeks of refrigerated storage. Also there were the most changes in physicochemical characteristics in the cornelian cherry treatments containing this strain, which is a marker of fermentative activity during the refrigerated storage.ConclusionThe results showed that cornelian cherry juice because of low pH and presence of inhibitor phenolic compounds has negative effect on viability of probiotics, especially industrial strains during the refrigerated storage.Keywords: Probiotics, Cornelian cherry, Anti, oxidant activity, Viability, Phenolic compounds -
Seaweeds have caused an emerging interest in the biomedical area, mainly due to their contents of bioactive substances which show great potential as anti-inflammatory, anti-microbial, anti-viral and anti-tumoral drugs. Despite the diversity in quality and quantity of the Mediterranean Tunisian coast flora, with its large contains of marine organisms and seaweeds, most of them have not yet been investigated for pharmacological and biological activities. Anti-oxidant, anti-inflammatory and anti-proliferative effects of the aqueous extracts (AQ) of three brown seaweed respectively, Cystoseira crinita (AQ-Ccri), Cystoseira sedoides (AQ-Csed) and Cystoseira compressa (AQ-Ccom) were investigated. Anti-oxidant activity was evaluated using the DPPH assay. Total phenolic contents were measured using Folin–Ciocalteu method. The anti-inflammatory activity of these extracts was determined in-vivo, using carrageenan induced rat paw oedema assay. The anti-proliferative activity was studied on normal cells (MDCK and rat fibroblast) and cancer (A549, MCF7 and HCT15) cell lines by the ability of the cells to metabolically reduce MTT formazan dyes, in comparison to a reference drug the Cisplatin. Results demonstrated that AQ-Ccri, AQ-Csed and AQ-Ccom extracts exhibited significant radical scavenging activity. AQ-Ccom extract had the highest total phenolic content. AQ-Ccri, AQ-Csed and AQ-Ccom extracts exhibited significant anti-inflammatory activity in a dose dependent manner by comparison to reference drugs. Moreover, AQ-Ccri, AQ-Csed and AQ-Ccom extracts showed an important anti-proliferative activity against both Human tumor cell lines HCT15 and MCF7. These pharmacological efficacies of these AQ- extracts of Cystoseira were positively correlated with their total phenol content and their good anti-oxidant activity. The purification and the determination of chemical structures of compounds of these active aqueous extracts are under investigation. It could have a promising role in the future medicine and nutrition when used as drug or food additive.Keywords: Anti, oxidant activity, Anti, proliferative activity, Anti, inflammatory activity, Cystoseira
-
زمینه و هدف
ترکیبات آنتی اکسیدانی از شیوع بیماری های مزمن و تخریب بسیاری از مواد غذایی جلوگیری می کنند، هدف این مطالعه ارزیابی میزان فنل و فلاونوئید تام و فعالیت آنتی اکسیدانی پوست درختان راش و بلوط بود.
روش بررسیدر این مطالعه تجربی، پس از تهیه پوست درختان راش و بلوط، عصاره های استنی به روش سوکسوله استخراج شدند. ابتدا میزان فنل و فلاونوئید تام عصاره ها اندازه گیری شده و سپس برای ارزیابی خواص آنتی اکسیدانی عصاره های استخراج شده از سه روش دی فنیل پیکریل هیدرازیل، قدرت احیا کنندگی و نیتریک اکساید استفاده شد.
یافته هامیزان فنل در پوست درخت راش بیشتر و فلاونوئید در بلوط بیشتر بود. نتایج آزمون به دام اندازی رادیکال های آزاد دی-فنیل پیکریل هیدرازیل نشان داد که غلظت مهار50 درصد در عصاره استنی پوست درختان راش و بلوط به ترتیب 92/19 و 33/7 میکروگرم بر میلی لیتر بود. هم چنین عصاره راش قدرت احیاکنندگی بیشتری نسبت به بلوط داشت. در آزمون به دام اندازی نیتریک اکساید، غلظت مهار50 درصد در عصاره استنی راش 98/23 و در عصاره بلوط 92/90 میکروگرم بر میلی لیتر محاسبه شد.
نتیجه گیریعصاره استنی پوست درختان در سه مدل مورد مطالعه، سطوح مختلفی از فعالیت آنتی اکسیدانی را نشان دادند. عصاره راش فعالیت آنتی اکسیدانی بهتری نسبت به عصاره بلوط داشت.
کلید واژگان: فعالیت آنتی اکسیدانی, فنل, فلاونوئید, راش, بلوطArmaghane-danesh, Volume:18 Issue: 2, 2013, PP 137 -145Background and AimAnti-oxidant compounds prevent prevalence of chronic diseases and food spoiling. The aim of this study was to evaluate the total phenolic and flavonoid content and antioxidant activity of beech and oak barks.
MethodsIn this experimental study، the skin of beech and oak trees were prepared and then acetone extraction was obtained using Soxhle method. At the beginning، total phenol and flavonoid of extracts were determined and the anti-oxidant properties of the extracts were then evaluated by three methods (methods Biphenyl Pykryl Hydrosol، regenerative power produced- and nitric oxide).
ResultsThe amount of phenolic was higher in bark of beech trees، but flavonoids were higher in oaks. The result of test to trap free radicals of Biphenyl Pykryl Hydrazyl showed the inhibitory concentration 50% of acetone extract of the bark of beech and oak، were 92. 19 and 33. 7 mg/L respectively. Beech extracts had greater regenerative power than oak. In Nitric oxide trap test acetone extract inhibited 50% in bark of beech trees was 98/23 and the oak extract was 92/90 mg/L respectively.
ConclusionAcetone extract of the bark in three models showed varying degrees of anti - oxidant activity. Beech extract had better antioxidant activity compared with oak extract.
Keywords: Anti, oxidant Activity, Phenols, Flavonoids, Beech, Oak
- نتایج بر اساس تاریخ انتشار مرتب شدهاند.
- کلیدواژه مورد نظر شما تنها در فیلد کلیدواژگان مقالات جستجو شدهاست. به منظور حذف نتایج غیر مرتبط، جستجو تنها در مقالات مجلاتی انجام شده که با مجله ماخذ هم موضوع هستند.
- در صورتی که میخواهید جستجو را در همه موضوعات و با شرایط دیگر تکرار کنید به صفحه جستجوی پیشرفته مجلات مراجعه کنید.