جستجوی مقالات مرتبط با کلیدواژه « phase separation » در نشریات گروه « پزشکی »

Despite unique characteristics of tahini, adequate biotechnical studies have not been carried out on tahini. In recent decades, functional food production such as probiotics has increased because of positive effects on the consumer health. Studies have verified that probiotics do not include appropriate survivability in fat ambiences. Hence, the present study was carried out for the first time with the objective of functional tahini production with appropriate survivability of its probiotics.

Probiotic tahini was produced using encapsulated and free Lactobacillus plantarum subspecies PTCC 1896. Additionally, effects of sorbitan three stearate and glycerol on the oil migration and rheology of tahini were assessed using rheometer and centrifuge. Water/oil emulsification method with sodium-alginate, modified starch and sesame oil was used for the probiotic encapsulation.

In the equal quantity of sorbitan three stearate and glycerol, sorbitan three stearate has shown further effects on oil migration while increased the viscosity significantly. After comparison of 0.25, 0.5 and 0.75% additions of the highlighted compounds to tahini for their effects on oil migration and viscosity, 0.5% glycerol was selected to increase the bacterial survivability. At the end of storage time, the most probiotic survivability belonged to tahini, which contained glycerol and encapsulated probiotics (1.2 × 107).

Lactobacillus plantarum subspecies PTCC 1896 encapsulation via emulsion method and addition of it to tahini containing  0.5% glycerol produced functional tahini with 106–107 CFU/g alive bacteria.

Tissue engineering using scaffold, cells, and growth factor creates the appropriate environment to repair, replace, or regenerate of failure or damaged tissue. Scaffolds play the role of ECM of normal tissue and support cell growth and differentiation. Electrospinning is a fast and efficient method that makes it possible to make nanofiber scaffolds. Phase separation is also a fast and inexpensive method and requires fewer tools.

In the present study, after obtaining the optimal factors in electrospinning and phase separation process, from gelatin polymer for nanofiber synthesis and from poly-L-lactic acid polymer (PLLA) and multi-wall carbon nanotubes for making porous and nanofiber conduits containing berberine (Beri) was used and evaluated by various tests.

The results of in vitro characterization tests indicated the suitability of channels containing PLA/MWCNTs / nanofiber gelatin with berberine. Functional tests (including SFI, Hot plate test) and histological tests are made of a nerve and muscle (including H&E staining) as well as weighing of gastrocnemius muscle. The defect showed a significant improvement in berberine-receiving rats compared with the negative control group and the berberine-free groups.

synthesis of porous and nanofiber conduits from gelatin and poly-L-lactic acid polymer with electrospinning and phase separation process provide potential use in peripheral nerve tissue engineering.