جستجوی مقالات مرتبط با کلیدواژه "rapid method" در نشریات گروه "پزشکی"
جستجوی rapid method در مقالات مجلات علمی
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Background and ObjectivesHaemophilus influenza is a human-restricted Gram-negative bacterium that is part of the normal nasopharyngeal flora of most humans. H. influenzae can cause diseases such as pneumonia, meningitis, bacteraemia, sinusitis and acute otitis media. Nonculture methods like PCR have become available during the last two decades, providing early and accurate diagnosis of bacterial diseases. The purpose of this study was to design a PCR assay for the rapid detection of H. influenzae bacterium.Materials and methodsIn this study the target gene was P6 so the specific primers were designed for it. The PCR reaction was set up on the DNA genomic of H. influenzae. To create a positive control, the PCR product was cloned in the pTZ57R/T vector and was transformed into E. coli JM107. The sensitivity of this assay was tested by a serial dilution of the positive control. A total of 72 clinical samples collected from patients with sinusitis were analyzed by PCR.ResultsPCR results showed a band of the expected size 273 bp. Sensitivity results indicated that the limit of detection of the assay was 1 CFU/ml. No amplification was observed after PCR with any of the microorganisms tested. There was 19 (26%) positive numbers of H. influenza in the samples in this study.ConclusionsThe PCR method showed to be rapid, sensitive, highly specific, and cheaper than commercial methods. The PCR methods could be easily adopted by public laboratories of developing countries for diagnostic purposes.Keywords: Haemophilus influenzae, rapid method, PCR
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Traditional microbiological methods tend to be labor-intensive and time-consuming. Rapid and novel methods in microbiological tests provide more sensitive, precise and reproducible results compared with conventional methods. In microbiology, the most rapid testing methods belong to the field of biotechnology such as PCR, ELISA, ATP bioluminescence and etc. Nevertheless impedance microbiology, biosensors and analytical procedures to determine microbial constituents are of significance. The present review article was conducted using internet databases and related scientific literatures and articles that provide information on developments in the rapid methods in microbiology. The main focus is on the application of rapid methods in microbial quality control of pharmaceutical products. Reviewed literature showed that rapid methods and automation in microbiology is an advanced area for studying and applying of improved methods in the early detection, and characterization of microorganisms and their products in food, pharmaceutical and cosmetic industrials as well as environmental monitoring and clinical applications. It can be concluded that rapid methods and automation in microbiology should continue as potent and efficient technologies to develop the novel tests to be performed in the future because of the ever-increasing concerns about the safety of food and pharmaceutical products. However the main issues to be considered are the scale up of developed methods and the regulatory requirements.Keywords: Microbial quality control, Sampling, Detection, Identification, Rapid method
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