جستجوی مقالات مرتبط با کلیدواژه "respiratory syndrome" در نشریات گروه "پزشکی"

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of coronavirus disease 2019 (COVID-19) as a pandemic infectious disease which has led to thousands of deaths around the world. The Coronaviridae family is the second cause of the common cold that targets human respiratory tracts. Specific diagnostic laboratory tests in addition to clinical investigations would be helpful in confirming COVID-19 in the early stages for controlling the disease. Upon SARS-CoV-2 infection, antibody responses are produced during the early phase of illness (>7 days), meanwhile, viral nucleic acid real-time reverse-transcription polymerase chain reaction (rRT-PCR) test is applied as the confirmatory assay in the first 5-6 days after the onset of illness. Due to the rise of antibodies, the viral nucleic acid represents a gradual decline. These laboratory tests may be considered valuable for monitoring the patient’s status to prevent the spreading of infections and keep him/her in quarantine. The results of molecular and serological assays revealed that whether the person is recovered and protected against disease. Furthermore, regarding the rise of antibody titer and undetectable viral RNA, it may be possible to make a decision about when the recovered people could back to work and social life.

This study was designed to investigate the frequency and tissue distribution of Ornithobacterium rhinotracheale (ORT) in turkeys with respiratory syndrome in Isfahan province.

Totally, samples were taken from the trachea, lung, air sac, infraorbital sinus, hock joint, blood of heart, brain, liver, spleen, intestine, and kidney of 30 turkey flocks. After DNA extraction, a 787 bp fragment of 16S rRNA gene of ORT was amplified.

The PCR results revealed that 53% of turkeys were infected by ORT. The results showed that although ORT was mainly found in the respiratory tract, it could be systemic and infect some other organs, including the joints, brain, liver, spleen, and blood of heart, but could not infect the intestines and kidneys.

Due to the lack of a clear pattern in tissue distribution of ORT among clinical samples, it seems that other factors play a role in ORT tissue distribution, such as dose, route, type of infection, and probably prevalent serotype.

The virus causing COVID-19 disease is known as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The disease spread rapidly and was transmitted like a contagious disease throughout China, and then it gradually spread in other parts of the world. Accordingly, the rapid and accurate detection of the SARS-CoV-2 virus plays an essential role in selecting timely treatments, saving lives, and preventing the spread of the disease. This study summarizes the methods used to identify coronavirus nucleic acid. The effectiveness of coronavirus nucleic acid detection kits by different samples and the performance of other diagnostic techniques are also addressed in this study. We searched Embase, Google Scholar, MEDLINE, Web of Science, Scopus, and PubMed databases as well as the references of all relevant articles in English published during 2019 - 2020 using keywords related to COVID-19, detection kits, and respiratory failure and proceedings from relevant conferences and congresses. The authors collected the relevant reports, and each of the authors independently reviewed the data published in different studies. The results of previous studies indicated that the diagnosis methods of the COVID-19 disease are the RT-PCR method, ELISA kits, quick tests, white blood cell count, C-reactive protein (CRP) levels, other laboratory factors and antigenic detection methods. Given the sensitivity and specificity of these methods at different periods using different samples, the disease interpretation can be performed accurately. The findings showed that proper laboratory equipment and appropriate laboratory kits are necessary for the rapid and precise identification of COVID-19.