جستجوی مقالات مرتبط با کلیدواژه « rich plasma » در نشریات گروه « پزشکی »

Osteoblasts’ activity is prerequisite for prevention from and treatment of apical periodontitis and a relatively high proportion of endodontically treated teeth will require retrograde treatment in future. Therefore, the aim of the present study was to evaluate the effect of platelet-rich plasma (PRP) on differentiation of stem cells into osteoblasts and osteoclasts.
Mesenchymal stem cells were isolated from human fetal umbilical cord and cultured on two polycaprolacton/hydroxyapatite (PCL/HA) polymer scaffolds. In addition to differentiation agents, 10% PRP was added to PRP containing subgroups. After 10 days, osteoblast differentiation was assessed evaluating the osteocalcin and osterix gene levels where, in the osteoclast differentiation group the expression of tartarate-resistant acid phosphatase (TRAP) gene was evaluated.
Expression of TRAP gene did not reveal any significant differences between the study and control groups. There was a significant difference in osterix expression between the control and the PRP-treated groups (p The results showed that PRP increased the osteoblastic differentiation, while it does not cause any significant increase in osteoclastic differentiation.

Despite the availability of a wide range of antibiotics, bacterial infections are among important challenges for the healthcare system. Therefore, the introduction of new antimicrobial preparations such as platelet-rich plasma (PRP) could be suggested theoretically as a preventive measure for bacterial infections. We aimed to evaluate the in vitro antibacterial activity of PRP against three common oral bacteria.
In vitro antibacterial activity of PRP against Streptococcus Mitis, Streptococcus Mutans, and Neisseria Lactamica as three common oral/dental bacteria was determined by disc diffusion method. Baseline antibacterial activity was assessed by measuring the diameter zone of inhibition after 24 hours of incubation at 37 °C.
PRP had strong in vitro antibacterial activity against Streptococcus Mitis, Streptococcus Mutans and Neisseria Lactamica with the mean zone of inhibition diameters of 6.73±0.52, 5.8±0.43 and 6.67±0.43 mm, respectively.
PRP is an effective antibacterial agent along with conventional antibiotic treatments against oral and periodontal infections.

Knee osteoarthritis is one of the most prevalent and disabling diseases worldwide, which decreases patients’ quality of life (QoL). However, pharmacological and non-pharmacological treatments, apart from complications, could not desirably control the disease.
The current study aimed at evaluating the effect of two methods of intra-articular injection of Platelet-Rich Plasma (PRP) and corticosteroid on pain control of knee osteoarthritis.
A randomized clinical trial was performed on 67- patients with grades II-III of knee osteoarthritis (OA) referring to pain clinic of a referral, university-affiliated hospital, Rasht, Iran, from April 2016 to June 2017. Patients were randomly divided into two groups: Triamcinolone (T) (n=34) and PRP (P) (n=33) by quadruple block. In the group T, 40 mg Triamcinolone and in the group P, PRP was injected intra-articularly, under ultrasound- guidance, once a month, for three consecutive months. Patients’ pain intensity was evaluated based on the Visual Analog Scale (VAS), and their outcome was determined based on the Knee injury and Osteoarthritis Outcome Score (KOOS) monthly for three consecutive months, as well as six months after the treatment.
There were no significant differences between the groups regarding demographic characteristics. VAS assessments indicated lower pain scores in the group P than group T; the difference between the groups was statistically significant two, three, and six months after the injections. In the group P, the mean initial VAS was 7.36 ± 0.92 compared with 7.12 ± 1.29 in the group T (P = 0.385). After six months, the scores dropped to 3.45 ± 0.86 and 4.81 ± 1.4, respectively (P = 0.0001). Examination of the KOOS parameters showed a significant association between treatment outcomes in the group P than the group T. Therefore, test showed a significant difference between the groups regarding relief of pain, improvement of symptoms and activities of daily living (ADL) two, three, and six months after treatment; there was also a significant difference between the groups in terms of the quality of life (QoL) and doing sport activities three and six months after the treatment (P The current study results showed that three intra-articular injections of Triamcinolone and PRP could reduce pain and improve articular function in patients with grades II-III knee osteoarthritis. However, pain relief and improvement in the outcomes were more effective and more prolonged secondary to PRP injections than corticosteroids

Intra-articular injections of platelet rich plasma (PRP) for the treatment of knee osteoarthritis have been shown to reduce pain and improve joint function. The aim of this study is to examine the joint function by adding intra-articular growth hormone to platelet rich plasma. This study was performed on the individuals with knee osteoarthritis and under ultrasound guidance..
Fifty four patients who were scheduled for ultra-sound guided intra-articular injection were enrolled in the study. The patients were randomly allocated to groups P (platelet rich plasma) and PS (platelet rich plasma and Somatropin). Group P and PS were injected with 5 mL of platelet rich plasma, and 4 IU growth hormone (Somatropin) added to platelet rich plasma, respectively. Intra-articular injection was performed in two steps; the onset of study and one month after. Knee joint function based on Western Ontario and McMaster osteoarthritis index (WOMAC) score at the baseline, 1 and 2 month later, and complications were evaluated..
WOMAC score in both groups has been significantly reduced after injections (P = 0.030). WOMAC score reduction in group PS in first month was significantly higher than group P, but in second month 2, the difference between two groups was not significant (P = 0.235). No complication was observed..
These results showed that adding growth hormone to platelet rich plasma for intra-articular injection improved function of the osteoarthritic knee joint in short period of time..

Plasma rich in growth factors (PRGF) and freeze‑dried bone allograft (FDBA) are shown to promote bone healing. This study was aimed to histologically and histomorphometrically investigate the effect of combined use of PRGF and FDBA on bone formation, and compare it to FDBA alone and control group.
The distal roots of the lower premolars were extracted bilaterally in four female dogs. Sockets were randomly divided into FDBA PRGF, FDBA, and control groups.
Two dogs were sacrificed after 2 weeks and two dogs were sacrificed after 4 weeks. Sockets were assessed histologically and histomorphometrically. Data were analyzed by Kruskal–Wallis test followed by Mann–Whitney U‑tests utilizing the SPSS software version 20. P While the difference in density of fibrous tissue in three groups was not statistically significant (P = 0.343), the bone density in grafted groups was significantly higher than the control group (P = 0.021). The least decrease in all socket dimensions was observed in the FDBA group. However, these differences were only significant in coronal portion at week 4. Regarding socket dimensions and bone density, the difference between FDBA and FDBA㴑 groups was not significant in middle and apical portions.
The superiority of PRGFᐰ overFDBA in socket preservation cannot be concluded from this experiment.

Repeated implantation failure (RIF) is a major challenge in reproductive medicine and despite several methods that have been described for management, there is little consensus on the most effective one.

This study was conducted to evaluate the effectiveness of platelet-rich plasma in improvement of pregnancy rate in RIF patients.

Twenty women with a history of RIF who were candidates for frozen-thawed embryo transfer were recruited in this study. Intrauterine infusion of 0.5 ml of platelet-rich plasma that contained platelet 4-5 times more than peripheral blood sample was performed 48 hrs before blastocyst transfer.

Eighteen participants were pregnant with one early miscarriage and one molar pregnancy. Sixteen clinical pregnancies were recorded and their pregnancies are ongoing.

According to this study, it seems that platelet-rich plasma is effective in improvement of pregnancy outcome in RIF patients.

The purpose of this study was to create biomaterial scaffolds like platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) containing stromal cell-derived factor-1 (SDF1) as a chemokine to induce hyaline cartilage regeneration of rabbit knee in a full thickness defect.
We created a full thickness defect in the trochlear groove of thirty-six bilateral knees of eighteen mature male rabbits. The knees were randomly divided into six groups (group I: untreated control, group II: PRP, group III: PRF, group IV: Gelatin䠱, group V: PRP䠱, and group VI: PRF䠱). After four weeks, the tissue specimens were evaluated by macroscopic examination and histological grading, immunofluorescent staining for collagen type II, and analyzed for cartilage marker genes by real-time PCR. The data were compared using statistical methods (SPSS 20, Kruskal-Wallis test, Bonferroni post hoc test and P Macroscopic evaluations revealed that international cartilage repair society (ICRS) scores of the PRF䠱 group were higher than other groups. Microscopic analysis showed that the ICRS score of the PRP group was significantly lower than other groups. Immunofluorescent staining for collagen II demonstrated a remarkable distribution of type II collagen in the Gel䠱, PRP䠱 and PRF䠱 groups compared with other groups. Real-time PCR analysis revealed that mRNA expression of SOX9 and aggrecan were significantly greater in the PRF䠱, PRP䠱, Gel䠱 and PRF groups than the control group (P Our results indicate that implantation of PRF scaffold containing SDF1 led to the greatest evaluation scores of full-thickness lesions in rabbits.

Pulp regeneration within the root canal of necrotic teeth is considered an ideal treatment to allow for continued root development and recover teeth vitality. This study aims to evaluate the inductive effect of platelet-rich plasma (PRP) on expression of angiogenesis factors and pulpal revascularization of immature necrotic teeth.
In this experimental animal study, we randomly divided 28 immature premolars from two mixed breed dogs into four groups, two experimental, negative and a positive control. Premolars in negative control group were left intact to develop normally. In the positive control and experimental groups, we removed the pulps and induced pulp necrosis, after which the chambers were sealed. Then, we applied the revascularization protocol in the experimental teeth located in the right quadrant. Two months later, the same protocol was applied to the left quadrant. The root canals were disinfected by irrigation with sodium hypochlorite (NaOCl) solution and application a triple antibiotic past. Following the induction of a blood clot (BC) inside the canal space, the coronal portion of the canals was assigned to either of two experimental groups: group 1 [BC㴓 mineral trioxide aggregate (MTA)], group 2 (BC㄰). Access cavities were sealed with a Glass Ionomer. The jaws that held the teeth were processed for histologic analysis of newly formed tissue and immunohistochemical evaluation according to vascular endothelial growth factor (VEGF) and factor VIII expressions in the canals.
Histological analysis demonstrated no significant difference in the formation of new vital tissue inside the root canals between groups1 (42.8%) and 2 (43.5%, P 0.05). Based on immunohistochemical evaluation, micro-vessel density (MVD) of the granulation tissues in both groups were similar and were higher compared with the normal pulp. We observed strongly positive expressions of VEGF and factor VIII in the stromal and endothelial cells, with severe intensity after one month. Both factors showed downregulation at three months postoperative.
PRP could not increase the formation of new vital tissue. The immunohistochemical results showed that VEGF and factor VIII played a pivotal role in the formation of new vessels inside the root canals of immature, non-vital teeth.

Platelet-rich plasma (PRP) is a human plasma product enriched by platelets, growth factors, and fibrinogen with high hemostatic and healing properties.
The aim of this study was to evaluate the effect of autologous PRP on wound healing in high-risk women undergoing cesarean sections.
Patients and In this balanced, randomized, and controlled trial, 140 patients were admitted to Arash women’s hospital, Tehran, Iran from May of 2013 to November of 2014 for elective cesarean surgery. The patients were randomly assigned into two groups. The intervention group received PRP after surgery, whereas the control group received the usual care. All patients were evaluated at baseline, five days, and eight weeks after the cesarean section. The primary endpoint used the REEDA scale for assessing the changes in wound healing. The secondary outcome measures used were the Vancouver scar scale (VSS) and the visual analog scale (VAS). All scale scores were analyzed using a repeated measures test for variance.
At the end of study, the PRP group showed a greater reduction in the edema ecchymosed discharge approximation (REEDA) score compared to the control group (85.5% reduction in the PRP group; 72% in the control group) (P It seems that applying PRP is an effective therapeutic approach for wound healing, and faster wound healing is expected due to the presence of more platelets and growth factors.

The aim of this study is to find out the spatial and temporal expression of TGF-b1 during the tendon healing, after application of Platelet Rich Plasma (PRP).
A patellar tendon defect model in rabbits was used for this purpose. 48 skeletally mature New Zealand White rabbits, weighing 3.5 kg, were used for this study. Equal numbers of animals from both groups were sacrificed at 4 different time points (1st, 2nd, 3rd, and 4th week). A full thickness patellar tendon substance in the right limb of each animal was excised from its central portion during the operation. PRP with a gel form was applied and filled the tendon defect in PRP group. No PRP was applied in the tendon defect of controls. Histological sections with hematoxylin-eosin and immunohistochemical sections with an anti-TGF-b1 primary antibody were made for the evaluation of the results.
A differentiation of the healing process was observed in the PRP group in comparison with the control group. TGF-b1 expression was detected in various cell populations (inflammatory cells, endothelial cells, macrophages, and tenocytes). Both cytoplasmic and nuclear expressions were present. The larger amounts of immunoexpression were localized in epitenon and in the repair site. PRP group showed stronger and more extensive staining at 1st and 2nd week (P Our study demonstrates that locally application of PRP result in an alteration of TGF-b1 expression during the healing of a patellar tendon defect.

Frozen shoulder is a glenohumeral joint disorder that perturbs movement because of adhesion and the existence of fibrosis in the shoulder capsule. Platelet-rich plasma can produce collagen and growth factors, which increases stem cells and consequently enhances the healing. To date, there is no evidence regarding the effectiveness of platelet-rich plasma in frozen shoulder. A 45-year-old man with shoulder adhesive capsulitis volunteered for this treatment. He underwent two consecutive platelet-rich plasma injections at the seventh and eighth month after initiation of symptoms. We measured pain, function, and ROM by the visual analogue scale (VAS), and scores from the Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire and goniometer; respectively. After the first injection, the patient reported 60% improvement regarding diurnal shoulder pain, and no night pains. Also, two-fold improvement for ROM and more than 70% improvement for function were reported. This study suggests the use of platelet-rich plasma in frozen shoulder to be tested in randomized trials.

Plasma rich in growth factors (PRGFs) has been recently proposed as an aid to enhance regeneration of osseous and epithelial tissues in oral surgery. The purpose of this study was to determine the effect of local application of Platelet Rich Plasma (PRP) on implant stability measured by periotest. A total of 24 implants were placed in mandible of 12 lower edentulous patients. In each patient, 2 implants were placed anterior to mental foramen in bilateral canine sites. One implant in each patient was dipped in autogenous PRP before insertion (test group), while the other implant was not embedded in PRP (control group). Repeated stability measurements were done by periotest on the day of surgery, 1, 2, 4 and 8 weeks after surgery. In both groups minimum periotest values (highest stability) was observed in the day of surgery and 8 weeks after surgery. The maximum periotest values (lowest stability) were observed in 4th week after surgery. Considering implant stability, no statistically significant differences was observed between test and control groups at any time (P>0.05). In PRP group, the difference in implant stability between the day of surgery to 2nd and 4th weeks were statistically significant (p

Platelet-rich plasma (PRP) is plasma of enriched platelets with high concentration of platelet granules and growth factors. The platelet growth factors have a great potential of wound and connective tissue healing used to treat cartilage lesions and retarding the progression of knee osteoarthritis. This study was designed to evaluate PRP injection results in knee osteoarthritis. Patients and This was a case-series study of 39 patients and all of them had three injections of PRP for the involved knee (every two weeks) and they were followed up at two weeks, two months and six months post-injection of PRP. All included patients filled the WOMAC (western Ontario and Mcmaster universities arthritis index) standardized and translated to our national language and culture. We analyzed the collected scores of before initiation of PRP injections, the second week, the second month and the sixth month after injections into the knees. Thirty-nine patients with a mean age of 55.24 ± 9.27 years (ranged 40 to 83) were evaluated in this study. Six patients (15.4%) were male and 33 patients (84.6%) female. There were significant differences in WOMAC score of patients in two months and six months after injection of PRP compared to before injection. PRP had a great clinical improvement in short-term follow-up for early stages of knee osteoarthritis.

Articular cartilage injuries of the knee are among the most debilitating injuries leading to osteoarthritis due to limited regenerative capability of cartilaginous tissue. The use of platelet concentrates containing necessary growth factors for cartilage healing has recently emerged as a new treatment method.. The efficacy of two types of different platelet concentrates were compared in the treatment of acute articular cartilage injuries of the knee in an animal model.. Eighteen adult Iranian mixed breed male dogs were used to conduct this experimental study. Full thickness articular cartilage defects (diameter 6 mm, depth 5 mm) were created in the weight bearing area of femoral condyles of both hind limbs in all dogs (n = 72). Twelve dogs were randomly selected to receive treatment and their right and left hind limb defects were treated by L-PRP and L-PRF implantation respectively, while no treatment was undertaken in six other dogs as controls. The animals were euthanized at 4, 16 and 24 weeks following surgery and the resultant repair tissue was investigated macroscopically and microscopically. At each sampling time, 4 treated dogs and 2 control dogs were euthanized, therefore 8 defects per group were evaluated.. Mean macroscopic scores of the treated defects were higher than the controls at all sampling times with significant differences (P < 0.05) observed between L-PRF treated and control defects (10.13 vs. 8.37) and L-PRP treated and control defects (10 vs. 8.5) at 4 and 16 weeks, respectively. A similar trend in mean total microscopic scores was observed with a significant difference (P < 0.05) between L-PRP treated and control defects at 4 (9.87 vs. 7.62) and 16 (13.38 vs. 11) weeks. No significant difference was observed between the platelet concentrate treated defects in either mean macroscopic scores or mean total microscopic scores.. Both L-PRP and L-PRF could be used to effectively promote the healing of articular cartilage defects of the knee.