جستجوی مقالات مرتبط با کلیدواژه « rosemary extract » در نشریات گروه « پزشکی »

The effect of conditioned medium on apoptosis and invasion of MCF-7 is still debated. Carnosic acid, a component of rosemary extract, is also reported to have anti-cancer property. Therefore, we studied the occurrence of apoptosis through AIF-dependent pathway in MCF-7 cells treated with conditioned medium and rosemary extract by evaluating the expression of AIF, P53, Bcl-2 and Bax genes.

Method and Materials:

In this studty, the experimental groups were: cultured MCF7 cells in medium containing serum (control), cultured cells in conditioned medium containing serum (CM group), cultured cells in medium containing different concentrations of rosemary extract (50 and 100 μg / ml) for 24 and 48 hours (treatment groups). The cell proliferation rate was studied by MTT test. Gene expression was assessed by RT-PCR.

The survival rate of cells treated by conditioned medium and serum for 24 and 48 h was significantly decreased as compared to control. Meanwhile, there was a significant increase in the survival rate of MCF-7 cells cultured with rosemary 50 and 100 μg / ml containing serum for 48 h, compared to group treated with conditioned medium and serum. A significant increase in P53, AIF and Bax gene expression, and also a significant decrease in Bcl-2 gene expression were observed in cells treated with conditioned medium and serum for 24 and 48h, as compared to the control. Rosemary at the concentration of 100 µg/ml and serum for 48 h significantly decreased P53, AIF and Bcl-2 genes and rosemary at concentration of 50 µg/ml containing serum for 24 and 48 h significantly decreased Bax gene expression compared to conditioned medium and serum group.

The AIF-dependent apoptotic pathway was activated in MCF-7 cells after treatment with conditioned medium 24 and 48 h and rosemary 100 containing serum after 48 h.

Bleeding following intubation and extubation after general anesthesia is a relatively common complication that is associated with a high prevalence in some surgeries such as breast surgeries. The use of traditional medicines has been welcomed by anesthesiologists due to their minor side effects. This study was performed with aim to investigate the effects of rosemary on bleeding and pain of intubation after general anesthesia for women candidates for breast surgery.

The clinical trial study was performed in 2017-2018 on 70 patients who need general anesthesia in Imam Reza Hospital, Tabriz. The subjects were randomly divided into two groups of intervention (inserting sterile gas impregnated with rosemary in the patient's throat) and control (inserting sterile gas impregnated with stilled water in the patient's throat). Observation of bleeding and pain due to intubation was measured by VAS instrument in the recovery and 2, 6, 24 hours after the surgery. Data were analyzed by SPSS statistical software (version 25) and independent t-test and chi-square test. P<0.05 was considered sttisticallyy significant.

Eleven patients (31.42%) in the intervention group and 14 (40%) in the control group had post-extubation bleeding (P = 0.309). Also, the number of pain due to intubation (P <0.05) and pain intensity (P >0.05) at different times showed no statistically significant difference between the two groups.

Sterile packs impregnated with rosemary extract could not prevent bleeding and pain due to intubation in women candidates for breast surgery.

Changes in the expression of pre-inflammatory cytokines play a peculiar role in the acceleration of brain aging. The consumption of some traditional herbs with antioxidant and anti-inflammatory properties(such as rosemary) and regular aerobic exercise are effective factors in the control of premature aging. The present study aimed to assess the effects of aerobic exercise and consumption of rosemary extract on Tumor necrosis factor-alpha  (TNF-α) and Interleukin 6 (IL-6) levels in the cerebellum tissue of old male rats.

A total of 40 male Wistar rats (18 months) weighing about 350–450 g were selected. They were randomly assigned to five groups: control, sham (treadmill off and distilled water gavage), practice (12 weeks, 5 days a week), supplement or extract (12 weeks of daily 100 mg/ kg gavage rosemary extract), and practice-supplement(extract). To evaluate the level of cytokines, the cerebellum tissue was quickly removed 24 h after the last practice session according to ethical principles. After homogenization, the levels of TNF-α and IL-6 were evaluated using enzyme-linked immunosorbent assay (ELISA technique). Data statistical analysis was carried out using one-way ANOVA and Tukey post-hoc tests.

As evidenced by the obtained results, a significant increase was observed in IL-6 level in the exercise and extract groups, compared to the sham and control groups (P< 0.05), as well as in the extract-practice group, compared to controls (P<0.01), and sham (P<0.001). On the other hand, TNF-α level was decreased in the treated groups, in comparison to the sham and control groups; however, this decrease was not significant.

Based on the results, aerobic exercise training along with rosemary extract consumption can be effective in the production and balance of pro-inflammatory cytokines in the cerebellum tissue of aging rats.

Aging is a phenomenon that, with a sequence of time-dependent changes, causes neurodegeneration and decreases the physiological function of the brain. Oxidative stress is one of the effective mechanisms in the neurodegeneration and aging process of the nervous system. Since the antioxidant effects of aerobic exercise and rosemary extract have been shown separately and mostly in the short term. In this study, we investigated the long-term effects of aerobic exercise and rosemary oral extract, separately and together, on the rate of the hippocampal neurodegeneration in old male rats.

Animals were treated for three months (5 days per week) with treadmill aerobic exercise and daily (once a day) oral administration (gavage) of 100 mg/kg rosemary extract (containing 40% carnosic acid). The number of intact and degenerated neurons in the hippocampus were counted by Nissl and fluorescence Fluoro-jade B staining methods, respectively.

The mean number of intact neurons in the CA1 region of the hippocampus showed significant increase in the exercise, exercise-rosemary (p<0.001) and rosemary (p<0.05) groups compared to the control groups (intact and vehicle). The number of degenerated neurons stained showed a significant decrease in the animals in exercise, rosemary and exercise-rosemary groups (p<0.001) compared to the control groups (intact and vehicle).

Aerobic exercise and consumption of rosemary extract (containing 40% carnosic acid) for three months, individually and in combination, were able to maintain neuronal density in the CA1 region of the hippocampus in old rats. Also, data analysis showed that long-term regular aerobic exercise had a stronger protective effect on maintaining hippocampal cell density than rosemary extract.