جستجوی مقالات مرتبط با کلیدواژه "sodium metabisulfite" در نشریات گروه "پزشکی"

This study investigates the potential effect of sub-chronic exposure to sodium metabisulfite (NaMBS) on the hippocampus and prefrontal cortex of female Wistar rats. 

A total of 24 adolescent female Wistar rats were randomly divided into four groups (6 rats each) as follows: Group 1 (control) received 0.5 mL of normal saline; group 2 was administered by 100 mg/kg of NaMBS; group 3 was issued by 300 mg/kg of NaMBS; group 4 received 500 mg/kg of NaMBS. The route of administration was oral for 28 days. After completing the administration phase, the Y-maze test was conducted. Subsequently, the rats were euthanized, and tissue samples from the prefrontal cortex and hippocampus were collected for biochemical assays, precisely measuring malondialdehyde (MDA) and acetylcholinesterase (AChE) levels, as well as histological studies, such as hematoxylin and eosin, and glial fibrillary acidic protein (GFAP). Meanwhile, the neuronal count was done. 

The learning and memory functions of rats in the treated and control groups were similar (number of alternations: P>0.05). The group treated with 500 mg/kg NaMBS presented indications of neurodegeneration in CA1 of the hippocampus and high glial fibrillary acidic protein-immunoreactivity but had no noticeable effects on layer II/III of the prefrontal cortex. Regardless of the dosage of NaMBS, malondialdehyde level was the same (P>0.05) for all groups; however, in the group that received 500 mg/kg NaMBS, the acetylcholinesterase level was significantly reduced (P˂0.05). 

This study revealed that while NaMBS can lead to neurodegeneration in the hippocampus of Wistar rats at 500 mg/kg, the prefrontal cortex remains resilient and spatial memory is unaffected, but a decrease in acetylcholinesterase activity raises cognitive concerns, emphasizing the need for cautious consideration of its use.

Sodium metabisulfite (SMB) is a frequently utilized as food preservative. While it is generally acknowledged to be safe, there have been concerns regarding its potential impacts. This study aimed to investigate the effects of sodium metabisulfite on the hormonal levels, ovarian and uterine histology, and oxidative stress markers in female Wistar rats.

Twenty-four adolescent female Wistar rats were randomly allocated into four groups of siz rats each: Group 1 (control) received 0.5mL normal saline; Group 2 was given 100 mg/kg SMB; Group 3 received 300 mg/kg SMB; and Group 4 was administered 500 mg/kg SMB. The administration was done orally over 28 days, followed by euthanasia for tissue collection. Blood samples were collected to assess the serum follicle stimulating hormone (FSH) and luteinizing hormone (LH), while ovary and uterus tissue samples were harvested for malondialdehyde (MDA) assays and histopathology. For histopathology, we used haematoxylin and eosin and periodic acid schiff staining.

The administration of SMB at doses of 300 and 500mg/kg had a notable impact on the hormone levels, particularly FSH and LH. The SMB doses also resulted in disrupted histo-architecture and altered glycogen expression in ovaries and uteri, as observed by histological examinations. Furthermore, SMB at 500mg/kg led to a significant increase in the oxidative stress marker malondialdehyde.

The SMB treatment affected FSH and LH levels, influencing ovarian and uterine structures. Disrupted structure and raised oxidative stress imply reproductive health risks. Further research is needed, including the effects of SMB on glycogen and FSH status.

Sulfites including Sodium Metabisulfite (SMB) are commonly used as food preservatives and pharmaceutical products. Despite their worldwide use, there is evidence suggesting their toxicity on human organs and tissues. The purpose of this study was to evaluate the effect of SMB with or without Zingiber officinale (ginger) extract on the rat ovary. 

A total of 32 adult, female Wistar rats were divided into four groups of eight each. They consisted of, a) control group, b) ginger group (500 mg/kg/day), c) SMB group (260 mg/kg/day), and d) combined SMB and ginger group at identical doses. After 28 days, the rats were sacrificed and the ovarian tissue Malondialdehyde (MDA), as a marker of lipid peroxidation was measured. The volume and weight of the ovaries and the number of follicles at different stages were counted by stereological methods. 

The SMB treatment caused a significant decrease in the ovarian volume and the number of follicles with simultaneous increase in the number of degenerate follicles (P≤0.001) and MDA level (P≤0.01). Ginger treatment of the rats exposed to SMB significantly increased the number of follicles at various stages and partially reversed the ovarian tissue level of MDA, compared to that in the control group (P≤0.05).

The SMB treatment induced structural changes in the rats’ ovaries and the concomitant treatment with ginger largely reversed the damages caused by SMB.

Sulphites are widely used as a preservative and antioxidant additives in the food and pharmaceutical industries. Many types of biological and toxicological effects of sulphites in multiple organs of mammals have been shown in previous studies.

The aim of this study was to investigate the effects of sodium metabisulfite (SMB) on testicular function and morphometric values of epididymis in adult male Wistar rats.

A total of 32 rats were randomly divided into four groups. The experimental groups received SMB at doses of 10 mg/kg (S10), 100mg/kg (S100), and 260 mg/kg (S260) while an equal volume of normal saline was administered to the control group via gavage. The rats were anaesthetized after 28 days and the left testis with the head of epididimis was excised following abdominal incision for histological observation using hematoxylin and eosin staining. Serum samples were collected for assay of testosterone level. The initial epididymis was analyzed for motility, morphology, and the number of sperms.

The results of this study showed that normal morphology, count, and motility of sperms and testosterone level were decreased in the SMB treated groups. In comparison with the control group, SMB resulted in a lower total number of spermatogonia, primary spermatocyte, spermatids, and Leydig cells.

It is suggested that SMB decreases the sperm production and has the potential to affect the fertility adversely in male rats.