جستجوی مقالات مرتبط با کلیدواژه "stomach neoplasm" در نشریات گروه "پزشکی"

Gastric cancer is considered as the fourth most common cancer and the second most common cause of cancer-related death around the world. Northern areas of Iran from NorthWest to NorthEast have high risk of this cancer. Genetic mutations and changing in expression level are the most effective factors in carcinogenesis and cancer progression. CFL1 is one of the genes that is affected by changes in the level of expression. CFL1 is isoform of the ADF/cofilin protein familyThe protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. In this research, we studied expression of this gene in intestinal samples of gastric adenocarcinoma.
Material and In this study, mRNA in intestinal type of gastric adenocarcinoma of 26 biopsy samples was extracted, then after cDNA synthesis, real-time polymerase chain reaction was performed to measure gene expression.
The rate of CFL1 gene expression in tumor samples significantly increased compared with healthy tumor margins (p This gene can be used as a biomarker in gastric cancer. Overall, the results of this study showed that the expression of CFL1 has significant association with the nature of cancer tumors. This implies that CFL1 can have high potential as novel targets for gene therapies in gastric cancer.

Gastric cancer is the first most common cancer death in Iran. There have been many efforts in finding the most effective proteins in this cancer. Using the proteins identified in gastric cancer combined with advanced computational tools in analyzing biological networks, we have developed a rational method in order to identify candidate proteins associated with this cancer.
In this study, The analytical procedure is performed with quantitative view. At the beginning we extracted the available studied proteins using a comprehensive literature search, Important proteins in gastric cancer were extracted from the available scientific articles, then the protein interaction databases were searched using Cytoscape MiMI plugin in order to draw the interaction network. Using the Centiscape plugin more key nodes were identified and the Degree, Stress, Betweenness and Closeness were examined. We next added the available expression data and recalculated the parameters.
Our beginning list was 72 known proteins involved in gastric cancer, after creating a comprehensive network using the earlier mentioned tools and databases a network with 1673 nodes was created. Examining the GO term using the BINGO plugin most of the proteins were involved in the regulatory processes (65007, 50789, and 50794). Results in the network core index showed that HNF4A and TAF1 were the major key proteins in the protein interaction network which can be greatly involved in the development of gastric cancer.
The proteins identified in this study can be used as diagnostic markers and therapeutic targets used in gastric cancer. The process presented in this study can be used to identify key targets in other diseases as well.

Gastric cancer is one of the most common and lethal malignancies in the world. All-trans retinoic acid (ATRA) has been widely used to treat cancers, most notably for the acute promyelocytic leukemia (APL) - as a differentiation inducer. Notch signaling plays important roles in cell proliferation and apoptosis. Aberrant activation of notch signaling pathway has been stated in different malignancies including gastric cancer. The aim of this study was to assess the possible effects of ATRA on cellular viability, cell cycle shift and apoptosis induction of gastric MKN-45 cell line. Moreover, alterations of notch1 and hes1 gene expression profiles after ATRA treatment have been considered.
Human gastric carcinoma cells (MKN-45) were treated with increasing concentrations of ATRA (2.5, 5, 10, 15, 20, 25 µM). The viability of cells was determined with 3-(4, 5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Caspase-Glo 3/7 assay kit was applied to measure the apoptosis. Flow-cytometry was employed to distinguish cells in different phases of cell cycle. Expression profiles for the notch1 and hes1 genes were evaluated by reverse transcriptase PCR (RT-PCR).
MTT assay showed that ATRA could diminish MKN-45 cell line viability rate; the most effective dose was 10 µM. Concentrations higher than 10 µM of ATRA had no significant effect on reducing cancerous cell viability. Furthermore, ATRA treatment significantly amplified caspase3/7activation. Flow-cytometry analyses showed significant accumulation of cells in G1 phase of cell cycle in ATRA treated group in compare to control. Following treatment with ATRA, showed a significant reduction in notch1and hes1 genes profile expression.
According to our findings, ATRA could exert its cytotoxic effects on gastric cancer MKN-45 cell line through reducing cellular viability and inducing apoptosis. Remaining at G1 phase of cell cycle and reducing notch1 and hes1 gene expressions suggest the antiproliferative activity of ATRA

Gastric cancer is the most prevalent cancer with poor survival in gastrointestinal tract. Caspase 3 and 9 play an important role in the development and progression of cancer. Polymorphisms in the genes for these enzymes can affect gene activity and thus may influence susceptibility to gastric cancer. In this study، caspase 3 and 9 genes polymorphisms in patients with gastric cancer were examined. In a case - control study، 100 patients with gastric cancer and 100 healthy individuals were evaluated in the region rs4647601: G> T for caspase-3 and -1263 A> G gene promoter for caspase 9. DNA extraction was performed from whole blood according to manufacture protocol. RFLP-PCR method was carrying out for detection of caspase 3 and 9 genes genotype in two groups. In this study، 143 men and 57 women were evaluated. All of them were selected from the same race and geographical area. The results indicated an increase of the mutant G allele in the control group، which leads to a decreasing in the incidence of gastric cancer (P<0. 0001، OR: 0. 096، (%0. 95CL) =0. 04-0. 23). It seems that screening of -1263 A> caspase 9 polymorphism could be a useful marker in personal sensitivity to gastric cancer and help to cancer treatment and prevention process. It is concluded that caspase gene variation may be a diagnostic factor in the gastric cancer.

Complications after gastrectomy have an important effect on all dimensions of quality of life in patients with gastric cancer. The purpose of this study was to define the effect of educating self-care program on the quality of life in patients with gastric cancer after gastrectomy in Tabriz Hospitals. Fifty four patients with gastric cancer undergoing gastrectomy were studied in two groups: experimental and control. Data were collected using three questionnaires: demographic, quality of life in patients with cancer, and quality of life in patients with gastric cancer. Experimental group was educated about self-care program face to face for 30 minutes. The quality of life in patients with gastrostomy in both groups before surgery and one month after discharge was assessed. Before and after educating self-care program, the overall quality of life based on QLQ-C30 questionnaire in experimental and control groups was not statistically significant, but in experimental group significant differences were observed in global quality of life (P=0.04), emotional function (P=0.004), and physical problems (P=0.03). The overall quality of life based on QLQ-STO22 questionnaire before and after the educating self-care program in control group was not significantly different, but in experimental group significant differences were observed (P=0.04). Also in this group, some dimensions such as dysphagia (P=0.01), stomachache (P=0.001) and emotional problem (P=0.04) the quality of life improved. The mean difference was not significant in overall QOL between two groups, before and after intervention. However, differences were significant in the emotional (P=0.006) and psychological dimension (P=0.007). Education of self-care program after gastrectomy was effective on improving overall QLQ based on QLQ-STO22 questionnaire in test group and also was effective in improving some dimensions of quality of life such as psychological, emotional and global QLQ and effective on reducing physical and eating problems.